2009
DOI: 10.1590/s1516-84842009005000067
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Analysis of manual reticulocyte counts in the clinical laboratories of Ponta-Grossa and Campos Gerais, PR, Brazil

Abstract: Reticulocytes are precursory to the erythrocyte cells in the blood. They are anucleated cells, more rounded in shape and 20% greater, in volume, than the erythrocytes. However, when stained with panoptic dyes (Romanowski) they produce polychromatic slides, due to the presence of mature red blood cells with hemoglobin, synthesized during maturation, and reticulocytes with ribonucleic acid residues. These residues are stained with new methylene blue or brilliant cresyl blue dyes which confer the characteristic a… Show more

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Cited by 7 publications
(3 citation statements)
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“…A cost analysis between the different reticulocyte count methods was conducted taking into consideration just the price of reagents, of the kit and their import tariff, with just one determination: using the imported reagent, there would be an approximate cost of $ 4.45 (R$ 8.28), considering the present currency. This result is similar to that demonstrated by Simionatto (2009) (19) , who used another type of imported reagent in his study. Then, according to the author, the cost of one determination with the used reagent would be an average of R$ 9.50.…”
Section: Discussion Of Resultssupporting
confidence: 92%
“…A cost analysis between the different reticulocyte count methods was conducted taking into consideration just the price of reagents, of the kit and their import tariff, with just one determination: using the imported reagent, there would be an approximate cost of $ 4.45 (R$ 8.28), considering the present currency. This result is similar to that demonstrated by Simionatto (2009) (19) , who used another type of imported reagent in his study. Then, according to the author, the cost of one determination with the used reagent would be an average of R$ 9.50.…”
Section: Discussion Of Resultssupporting
confidence: 92%
“…9,14 Após 14 anos da implantação de um programa de controle de qualidade externo de proficiência em hematologia, aumentaram de 40% para 94,7% os laboratórios indianos que apresentavam índices aceitáveis de resultados para diversas determinações hematológicas, inclusive contagem de reticulócitos. 15 O trabalho de Simionatto e colaboradores, 16 publicado neste volume da Revista Brasileira de Hematologia e Hemoterapia, apresenta um estudo sobre a variação interobservadores para a contagem visual de reticulócitos, mostrando que dois examinadores experimentados e que seguiram rigorosamente os critérios estabelecidos, apresentaram estreita correlação entre seus resultados, enquanto outros examinadores participantes, que atuaram de acordo com sua rotina…”
unclassified
“…No entanto, sua efetividade demonstrada nesses trabalhos corrobora com nossos resultados, que demonstraram alta interação e degradação do LPS in vitro, mesmo em baixas concentrações. Ainda assim, estudos in vivo utilizando este FS são necessários para avaliar mais minuciosamente sua biocompatibilidade e toxicidade, uma vez que seu uso esteve sempre limitado a caracterizar resíduos de RNA intracitoplasmático em reticulócitos, nas áreas de histopatologia e citopatologia (Simionatto et al, 2009;Okoroiwu et al 2015).…”
Section: Discussionunclassified