Analysis of Major Polyphenolic Compounds of Cydonia oblonga Miller (Quince) Fruit Extract by UPLC-MS/MS and Its Effect on Adipogenesis in 3T3-L1 Cells

Abstract

Cydonia oblonga miller (quince) plant serves as a potential folk medicine for treating hypertension and cardiovascular diseases in China. However, to the best of our knowledge, no study has been conducted on the polyphenolic profile and anti-adipogenic effect of quince fruit grown in China. In the current study, we aimed to investigate the quince fruit extract’s major phenolic compounds, evaluate their antioxidant activity, and examine their effect on adipogenesis in 3T3-L1 cells. A rapid and sensitive analytical method was established for the simultaneous determination of major polyphenolic compounds by using ultra-pressure liquid chromatography coupled with a triple quadrupole mass spectrometer (UPLC-MS/MS). Among the 10 compounds, the cryptochlorgenic acid was noticed as the most abundant compound of both purified (242.44 ± 0.73 µg/mg dw) and unpurified extract (3.37 ± 0.01 µg/mg dw) followed by quercetin 3-rutinoside and chlorogenic acid. Alternatively, both extracts possessed a high quantity of phenolic acids (purified extract = 483.10 ± 5.16 µg/mg dw and unpurified extract = 7.89 ± 0.02 µg/mg dw). The purified extract exhibited a strong antioxidant capacity (DPPH: EC50 = 3.316 µg/mL, ABTS: EC50 = 36.38 µg/mL) as compared to the unpurified extract. Additionally, our results also showed that the extract at 100 µg/mL significantly suppressed the preadipocyte differentiation and decreased the lipid droplets up to 69% in mature adipocytes. The present study highlights an accurate and fast detection method for quince fruit extract polyphenolic compounds with its antioxidant and antiadipogenic effects. The study also provides the necessary information for the rational development and utilization of quince fruit extract as a source of phytochemicals.

Gauging Quince Phytonutrients and Its 4% Emulgel Effect on Amplifying Facial Skin Moisturizing Potential

“…The Cydonia oblonga fruit is reported to be a rich source of polyphenolic constituents like chlorogenic acid, quercetin, caffeic acid and kaempferol [11][12][13]. In the current study, the total phenolic content of Cydonia oblonga (fresh peel and pulp) was 25.48 ± 1.52 (mg GAE/g of sample) and the total flavonoid content of Cydonia oblonga (fresh peel and pulp) was 14.76 ± 0.84.…”

Cydonia oblonga Miller fruit extract exerts an anti-obesity effect in 3T3-L1 adipocytes by activating the AMPK signaling pathway

“…2 ). Other studies in which 3T3-L1 cells were treated with various polyphenolic compounds consistently reported a reduction in cell number and size and an inhibition of the rate of differentiation of preadipocytes into adipocytes [ 30 31 ]. In a previous study, COME had a particularly high chlorogenic acid content among the polyphenolic compounds, suggesting that this was the main component exhibiting anti-obesity effects [ 24 ].…”

Utilization of Quince (Cydonia oblonga) Peel and Exploration of Its Metabolite Profiling and Cardioprotective Potential Against Doxorubicin-Induced Cardiotoxicity in Wistar Rats

“…The compounds obtained from plants such as phenolics are reported to be of great pharmacological importance having antioxidant, antihypertensive, antiatherosclerosis, antidiabetic, and anticancer properties. They are also said to alleviate the risk of CVD . The consumption of bioactive-rich compounds helps reduce the oxidative stress and hence aids in the reduction of chronic diseases.…”

“…The reported pharmacological uses of quince fruit include anti-inflammatory, antidiabetic, antiatherosclerosis, antihypertensive, etc . In traditional Uyghur medicine, quince is reported as a cure for CVD, and in Iranian medicine, it is reported as a cure for liver disease, headache, and nausea. , The cardioprotective effect of quince has been well established by animal studies. Quince leaves are reported to offer a cardioprotective effect against DOX-induced toxicity.…”

“…They are also said to alleviate the risk of CVD. 7 The consumption of bioactive-rich compounds helps reduce the oxidative stress and hence aids in the reduction of chronic diseases. Quince ( Cydonia oblonga) , belonging to the family Rosaceae, is an underutilized fruit that is promulgated to be a rich source of bioactive compounds.…”