Analysis of Lung Metastases in Patients with Primary Extremity Sarcoma

Songür, Necla; Dinç, Meral; Özdilekcan, Çiğdem; Eke, Sefa; Ok, Uğur; Öz, Murat

doi:10.1080/13577140310001607284

Cited by 13 publications

(11 citation statements)

References 17 publications

(21 reference statements)

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“…In carcinomas, the metastatic progression requires trans-differentiation of epithelial to mesenchymal phenotype, a process releasing cells from adherent junctions and increasing their ability to migrate through extracellular matrix (2). This, however, is not the rule in sarcomas, which usually do not form metastases despite the mesenchymal character of sarcoma cells (4). Additional changes are necessary for the sarcoma metastasis and the knowledge of this regulatory level could contribute to our incomplete understanding of the metastatic propensity and development of effective therapeutics (5).…”

Section: Introductionmentioning

confidence: 99%

Downregulation of HOPX Controls Metastatic Behavior in Sarcoma Cells and Identifies Genes Associated with Metastasis

Kovářová

Plachý

Kosla

et al. 2013

Molecular Cancer Research

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Comparing the gene expression profiles of metastatic and nonmetastatic cells has the power to reveal candidate metastasis-associated genes, whose involvement in metastasis can be experimentally tested. In this study, differentially expressed genes were explored in the v-src-transformed metastatic cell line PR9692 and its nonmetastatic subclone PR9692-E9. First, the contribution of homeodomain only protein X (HOPX) in metastasis formation and development was assessed. HOPX-specific knockdown decreased HOPX expression in the nonmetastatic subclone and displayed reduced cell motility in vitro. Critically, HOPX knockdown decreased the in vivo metastatic capacity in a syngeneic animal model system. Genomic analyses identified a cadre of genes affected by HOPX knockdown that intersected significantly with genes previously found to be differentially expressed in metastatic versus nonmetastatic cells. Furthermore, 232 genes were found in both screens with at least a two-fold change in gene expression, and a number of high-confidence targets were validated for differential expression. Importantly, significant changes were demonstrated in the protein expression level of three metastaticassociated genes (NCAM, FOXG1, and ITGA4), and knockdown of one of the identified HOPX-regulated metastatic genes, ITGA4, showed marked inhibition of cell motility and metastasis formation. These data demonstrate that HOPX is a metastasis-associated gene and that its knockdown decreases the metastatic activity of v-src-transformed cells through altered gene expression patterns.

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Section: Introductionmentioning

confidence: 99%

Downregulation of HOPX Controls Metastatic Behavior in Sarcoma Cells and Identifies Genes Associated with Metastasis

Kovářová

Plachý

Kosla

et al. 2013

Molecular Cancer Research

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“…In contrast, a study by Songur et al revealed malignant fibrous histiocytoma (22.2%), osteosarcoma (18.8%), and liposarcoma (15.5%) as the three commonest metastatic pulmonary lesions. [10] In another study amongst soft tissue sarcoma, leiomyosarcoma was the commonest (21%) type followed by malignant fibrous histiocytoma (18%), liposarcoma (12%) and synovial sarcoma (14%). [4] Previous studies have not demonstrated a relationship between histology of the metastatic lesion and outcome although some have shown a poor prognosis with liposarcoma (median survival 9.8 months).…”

Section: Discussionmentioning

confidence: 99%

A clinico-morphological study of 95 cases of sarcomas with metastases to the lungs

et al. 2011

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BACKGROUND: Metastasis of soft tissue sarcoma most commonly occurs to the lungs. There are very few studies on histology of pulmonary metastatectomy and hardly any wherein the histology of the primary tumor has been compared with the metastasis. AIMS AND OBJECTIVES: To review histologically all metastatic sarcomas to lung and compare with the primary where available. MATERIALS AND METHODS: Ninety-five patients with pulmonary metastases from sarcoma were analyzed histologically for type of sarcoma, chemotherapy-related changes, and changes in adjacent lung. Various clinical parameters like laterality, multiplicity, and interval between primary and metastasis were also studied. RESULTS: Osteosarcoma constituted half of the metastatic sarcomas (48 cases, 50.5%) followed by synovial sarcoma (16 cases, 16.8%) and high grade spindle cell sarcoma-NOS (10 cases, 10.5%). The histology of primary and the metastases was similar in 60% of cases of osteosarcoma. CONCLUSIONS: Osteosarcoma is the commonest metastatic sarcoma to the lung. There is often a change to fibroblastic histology in patients of conventional osteosarcoma treated with chemotherapy.

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“…Interestingly the lung is the most common site for systemic sarcoma metastases because of the substantial vasculature that feeds into this organ, in addition to particular tropic factors (4). Other cancers that often metastasize to the lung include breast, colon, prostate, bladder, and various neuroblastomas (5). …”

Section: Introductionmentioning

confidence: 99%

Strain-Specific Susceptibility for Pulmonary Metastasis of Sarcoma 180 Cells in Inbred Mice

Vikis¹,

Jackson²,

Krupnick³

et al. 2010

Cancer Research

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Most cancer deaths are a result of metastasis. To extend our understanding of the factors that influence the process, we aimed to developed a mouse model of pulmonary metastasis that can be assayed in multiple inbred mouse strains for further use in identification of host genetic variants that influence metastasis. We utilized intravenous injection of Sarcoma 180 (S180) cells, which can be tracked and quantified by bioluminescence imaging. We observed growth of S180 cells solely in the lung and observed a wide range of pulmonary metastasis among inbred mouse strains. Interestingly we noted that the BTBRT+tf/J strain exhibited complete clearance and provide evidence that the mechanism of resistance may involve immune factors, as strains subject to whole body irradiation are significantly more susceptible to tumor growth. One possible mechanism of resistance to pulmonary metastasis in BTBRT+tf/J mice may require T-cell function. Our experiments present a new mouse model for further characterization of the genetics and mechanisms of pulmonary metastasis.

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Analysis of Lung Metastases in Patients with Primary Extremity Sarcoma

Cited by 13 publications

References 17 publications

Downregulation of HOPX Controls Metastatic Behavior in Sarcoma Cells and Identifies Genes Associated with Metastasis

Downregulation of HOPX Controls Metastatic Behavior in Sarcoma Cells and Identifies Genes Associated with Metastasis

A clinico-morphological study of 95 cases of sarcomas with metastases to the lungs

Strain-Specific Susceptibility for Pulmonary Metastasis of Sarcoma 180 Cells in Inbred Mice

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