Analysis of longitudinal data from animals with missing values using SPSS

Duricki, Denise; Soleman, Sara; Moon, Lawrence

doi:10.1038/nprot.2016.048

Cited by 90 publications

(69 citation statements)

References 21 publications

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“…15 Random-effects models were constructed with FTS as dependent variable; subject ID as random factor; and group, time, and group by time interaction as fixed factors. To evaluate effects of viral vector injection or DREADD expression on seizure activity, FTS was compared before and 3 weeks after injection with rAAV in four mice (see higher) using a paired t-test.…”

Section: Effect On Spontaneous Hippocampal Seizuresmentioning

confidence: 99%

“…First, the effects of the highest CNO and clozapine doses were compared between DREADD and non-DREADD animals using the MIXED procedure in IBM SPSS Statistics. 15 Random-effects models were constructed with FTS as dependent variable; subject ID as random factor; and group, time, and group by time interaction as fixed factors.…”

Section: Effect On Spontaneous Hippocampal Seizuresmentioning

confidence: 99%

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Long‐term chemogenetic suppression of spontaneous seizures in a mouse model for temporal lobe epilepsy

et al. 2019

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Objective: More than one-third of patients with temporal lobe epilepsy (TLE) continue to have seizures despite treatment with antiepileptic drugs, and many experience severe drug-related side effects, illustrating the need for novel therapies. Selective expression of inhibitory Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) allows cell-type-specific reduction of neuronal excitability. In this study, we evaluated the effect of chemogenetic suppression of excitatory pyramidal and granule cell neurons of the sclerotic hippocampus in the intrahippocampal mouse model (IHKA) for temporal lobe epilepsy. Methods: Intrahippocampal IHKA mice were injected with an adeno-associated viral vector carrying the genes for an inhibitory DREADD hM4Di in the sclerotic hippocampus or control vector. Next, animals were treated systemically with different single doses of clozapine-N-oxide (CNO) (1, 3, and 10 mg/kg) and clozapine (0.03 and 0.1 mg/kg) and the effect on spontaneous hippocampal seizures, hippocampal electroencephalography (EEG) power, fast ripples (FRs) and behavior in the open field test was evaluated. Finally, animals received prolonged treatment with clozapine for 3 days and the effect on seizures was monitored. Results: Treatment with both CNO and clozapine resulted in a robust suppression of hippocampal seizures for at least 15 hours only in DREADD-expressing animals. Moreover, total EEG power and the number of FRs were significantly reduced. CNO and/or clozapine had no effects on interictal hippocampal EEG, seizures, or locomotion/anxiety in the open field test in non-DREADD epileptic IHKA mice. Repeated clozapine treatment every 8 hours for 3 days resulted in almost complete seizure suppression in DREADD animals. Significance: This study shows the potency of chemogenetics to robustly and sustainably suppress spontaneous epileptic seizures and pave the way for an epilepsy therapy in which a systemically administered exogenous drug selectively modulates specific cell types in a seizure network, leading to a potent seizure suppression devoid of the typical drug-related side effects. | 2315 DESLOOVERE Et aL.

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Section: Effect On Spontaneous Hippocampal Seizuresmentioning

confidence: 99%

Section: Effect On Spontaneous Hippocampal Seizuresmentioning

confidence: 99%

Long‐term chemogenetic suppression of spontaneous seizures in a mouse model for temporal lobe epilepsy

et al. 2019

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“…For sessions that didn’t include any data, a non-zero identifier was automatically placed for mean turn angle and success rate. This non-zero identifier is called a NaN, or “Not-a-Number,” and is commonly used to represent missing data (Duricki et al 2016). After keeping or discarding files, the Annotate Now option was chosen from the next prompt (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…However, after injury, a subject may be very impaired and not engage with the task. In these cases, the success rate and turn angle is recorded as a “Not-a-Number” (NaN), or the average of the success rate and turn angle of the trials present in the file (Duricki et al 2016). …”

Section: Methodsmentioning

confidence: 99%

Dexterity: A MATLAB-based analysis software suite for processing and visualizing data from tasks that measure arm or forelimb function

Butensky

Sloan

Meyers

et al. 2017

Journal of Neuroscience Methods

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Background Hand function is critical for independence, and neurological injury often impairs dexterity. To measure hand function in people or forelimb function in animals, sensors are employed to quantify manipulation. These sensors make assessment easier and more quantitative and allow automation of these tasks. While automated tasks improve objectivity and throughput, they also produce large amounts of data that can be burdensome to analyze. We created software called Dexterity that simplifies data analysis of automated reaching tasks. New Method Dexterity is MATLAB software that enables quick analysis of data from forelimb tasks. Through a graphical user interface, files are loaded and data are identified and analyzed. These data can be annotated or graphed directly. Analysis is saved, and the graph and corresponding data can be exported. For additional analysis, Dexterity provides access to custom scripts created by other users. Results To determine the utility of Dexterity, we performed a study to evaluate the effects of task difficulty on the degree of impairment after injury. Dexterity analyzed two months of data and allowed new users to annotate the experiment, visualize results, and save and export data easily. Comparison with Existing Method(s) Previous analysis of tasks was performed with custom data analysis, requiring expertise with analysis software. Dexterity made the tools required to analyze, visualize and annotate data easy to use by investigators without data science experience. Conclusions Dexterity increases accessibility to automated tasks that measure dexterity by making analysis of large data intuitive, robust, and efficient.

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“…An unstructured linear mixed effects model was applied to examine the main effect of repeated stimulation (second neutral and conditioned) and interaction effect with different condition modalities (warm, cool, interlaced warm and cool). The advantage of the LMM is that one can account for missing data when analyzing longitudinal data47. From this model, we were most interested to determine if conditioning differentially impacted CHEPs outcomes (i.e., interaction effect).…”

Section: Methodsmentioning

confidence: 99%

Thermal grill conditioning: Effect on contact heat evoked potentials

Jutzeler

Warner

Wanek

et al. 2017

Sci Rep

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The ‘thermal grill illusion’ (TGI) is a unique cutaneous sensation of unpleasantness, induced through the application of interlacing warm and cool stimuli. While previous studies have investigated optimal parameters and subject characteristics to evoke the illusion, our aim was to examine the modulating effect as a conditioning stimulus. A total of 28 healthy control individuals underwent three testing sessions on separate days. Briefly, 15 contact heat stimuli were delivered to the right hand dorsum, while the left palmar side of the hand was being conditioned with either neutral (32 °C), cool (20 °C), warm (40 °C), or TGI (20/40 °C). Rating of perception (numeric rating scale: 0–10) and evoked potentials (i.e., N1 and N2P2 potentials) to noxious contact heat stimuli were assessed. While cool and warm conditioning decreased cortical responses to noxious heat, TGI conditioning increased evoked potential amplitude (N1 and N2P2). In line with other modalities of unpleasant conditioning (e.g., sound, visual, and olfactory stimulation), cortical and possibly sub-cortical modulation may underlie the facilitation of contact heat evoked potentials.

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Analysis of longitudinal data from animals with missing values using SPSS

Cited by 90 publications

References 21 publications

Long‐term chemogenetic suppression of spontaneous seizures in a mouse model for temporal lobe epilepsy

Long‐term chemogenetic suppression of spontaneous seizures in a mouse model for temporal lobe epilepsy

Dexterity: A MATLAB-based analysis software suite for processing and visualizing data from tasks that measure arm or forelimb function

Thermal grill conditioning: Effect on contact heat evoked potentials

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