Analysis of linear plasmids isolated from Streptomyces: Association of protein with the ends of the plasmid DNA

Hirochika, Hirohiko; Sakaguchi, Kenji

doi:10.1016/0147-619x(82)90027-0

Cited by 90 publications

(72 citation statements)

References 16 publications

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“…Physical analysis revealed that pSLA2 is 17 kb in size and is structurally similar to adenovirus and bacteriophage f29 DNA; TIRs are present at both ends and a terminal protein is bound to the 5¢ ends. 40,41 However, it was later found that pSLA2 was not involved in the production of lankacidin or lankamycin. The application of PFGE to strain 7434AN4 revealed two linear plasmids, pSLA2-L and pSLA2-M, in addition to pSLA2-S (¼pSLA2) 4 (Table 1).…”

Section: Psla2-l In S Rocheimentioning

confidence: 99%

Giant linear plasmids in Streptomyces: a treasure trove of antibiotic biosynthetic clusters

Kinashi

2010

J Antibiot

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Many giant linear plasmids have been isolated from Streptomyces by using pulsed-field gel electrophoresis and some of them were found to carry an antibiotic biosynthetic cluster(s); SCP1 carries biosynthetic genes for methylenomycin, pSLA2-L for lankacidin and lankamycin, and pKSL for lasalocid and echinomycin. Accumulated data suggest that giant linear plasmids have played critical roles in genome evolution and horizontal transfer of secondary metabolism. In this review, I summarize typical examples of giant linear plasmids whose involvement in antibiotic production has been studied in some detail, emphasizing their finding processes and interaction with the host chromosomes. A hypothesis on horizontal transfer of secondary metabolism involving giant linear plasmids is proposed at the end.

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Section: Psla2-l In S Rocheimentioning

confidence: 99%

Giant linear plasmids in Streptomyces: a treasure trove of antibiotic biosynthetic clusters

Kinashi

2010

J Antibiot

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“…The plasmid DNA band appeared when the preparations were treated with Pronase before extraction with phenol/chloroform, or even without this last extraction. These results suggest firm binding of a protein to pFSl DNA, as occurs with pSLA2 (Hirochika & Sakaguchi, 1982). We further examined the location of the protein on pFSl by testing the susceptibility of the plasmid to 3' and 5' exonucleases.…”

Section: Association Of a Protein With The Ends Of The Plasmid D N Amentioning

confidence: 99%

“…Terminal proteins have been reported to be essential for DNA replication in phage $29 (Salas et al, 1978), in adenoviruses (Sakaguchi, 1990), and in pSLA2 DNA from Streptomyces (Hirochika & Sakaguchi, 1982).…”

Section: Association Of a Protein With The Ends Of The Plasmid D N Amentioning

confidence: 99%

“…The presence of a protein at the ends of the pFSl molecule was examined by reduction of the electrophoretic mobility of DNA in agarose gels, as previously observed for other linear extrachromosomal DNA elements (Hirochika & Sakaguchi, 1982;Ligon et al, 1989 ;Kinashi & Shimaji-Murayama, 1991). Cleared lysates containing pFSl or pSLA2 (linear plasmid used as a positive control) were prepared without Pronase treatment and were extracted with phenol/chloroform (1 : 1, v/v).…”

Section: Association Of a Protein With The Ends Of The Plasmid D N Amentioning

confidence: 99%

“…The linear form is shared with various transposons (Grindley & Reed, 1985), adenoviruses (Tamanoi & Stillman, 1983) and the bacteriophage 429 from Bacillus subtilis (Escarmis & Salas,198 1). Among eubacteria, Streptomyces (Hayakawa et al, 1979;Hirochika & Sakaguchi, 1982;Hirochika et al, 1984;Kinashi et al, 1987;Kinashi & Shimaji-Murayama, 1991), Nocardia (Kalkus et al, 1990) and Borrelia (Barbour & Garon, 1987) seem to be the only genera having strains that harbour linear plasmids, whereas the great majority of plasmids described in other genera are found as covalently closed circular DNA molecules. Many Bacillus strains carry endogenous plasmids, several of which do not express useful or identifiable genetic markers (Lovett & Bramucci, 1975;Tanaka & Koshikawa, 1977;Tanaka & Sakaguchi, 1978;Uozumi et al, 1980;Gryczan, 1982).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and partial characterization of a new linear DNA plasmid isolated from Bacillus polymyxa SCE2

Rosado

Seldin

1993

Journal of General Microbiology

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A plasmid, designated pFS1, has been identified for the first time in a strain of Bacillus polymyxa. The plasmid is 16 kb in length. It is sensitive to digestion by DNAase I, restriction endonucleases and exonuclease 111, but resistant to RNAase A and I-exonuclease. A restriction map of the plasmid has been constructed, demonstrating its linear nature. The linear DNA molecules of pFSl are attached at their 5' ends to a protein which can be cleaved by Pronase. No homology was detected between pFSl and chromosomal DNAs from a number of different B. pok'ymyxa strains. Also, hybridization between pFSl and total DNA from B. pok'ymyxa SCE2 plasmid-cured derivatives does not occur. The plasmid seems to be cryptic since B. polymyxa strains harbouring pFSl and the cured derivatives have the same phenotype.

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A linear DNA plasmid from Streptomyces rochei with an inverted terminal repetition of 614 base pairs.

Hirochika¹,

Nakamura²,

Sakaguchi³

1984

The EMBO Journal

109

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The terminal structure of a linear plasmid pSLA2, which was isolated from Streptomyces rochei, was analysed. The 5' ends of pSLA2 DNA were blocked by the association of a protein probably covalently bonded with the DNA. This block is removed by alkali treatment and blunt ends with 5' -phosphate and 3' -hydroxy termini were released. The two terminal fragments of pSLA2 were cloned and the nucleotide sequence was determined. An inverted terminal repetition of 614 bp was found along with the presence of further interrupted homologous sequences beyond this area up to 800 bp. These are the first inverted terminal repeat sequences found in microbial linear plasmids.

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Analysis of linear plasmids isolated from Streptomyces: Association of protein with the ends of the plasmid DNA

Cited by 90 publications

References 16 publications

Giant linear plasmids in Streptomyces: a treasure trove of antibiotic biosynthetic clusters

Giant linear plasmids in Streptomyces: a treasure trove of antibiotic biosynthetic clusters

Isolation and partial characterization of a new linear DNA plasmid isolated from Bacillus polymyxa SCE2

A linear DNA plasmid from Streptomyces rochei with an inverted terminal repetition of 614 base pairs.

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