The malfunctioning heart valve will compromise the cardiovascular circulation of the human body. Given that artificial valves can be used to replace a disabled one, a feasible in vitro evaluation system is important before any possible implantation. In this study, two types of transcatheter heart valves, namely, V-A (diameter: 23 mm) and V-B (diameter: 25 mm), are tested and compared by an in vitro experiment that incorporates a circulation system and a particle image velocimetry measuring module. Three dynamical indices, namely, the time-averaged wall shear stress, oscillatory shear index, and relative residence time, are applied for the evaluation. The results show that compared with V-B, V-A leads to lower probabilities of vessel wall damage and thrombosis formation, especially when the flow rate is close to that of the human body (4 l/min). Meanwhile, an independent analysis, which mainly concerns the mean pressure difference and regurgitation volume across the two ends of the valve, demonstrates that V-A has a better performance than V-B, thus verifying the above-mentioned analysis from the perspective of fluid dynamics. Therefore, the present in vitro measuring system is considered effective in evaluating the valve functionality.