Analysis of latex protein content by liquid chromatography coupled with tandem mass spectrometry (HPLC/MS/MS)

Abstract

Quantification of raw latex and latex glove proteins was carried out by liquid chromatography coupled with tandem mass spectrometry.

Identification of proteins, drying oils, waxes and resins in the works of art micro‐samples by chromatographic and mass spectrometric techniques

“…Method LOD and LOQ values (the lowest amount of AA detectable in the sample) listed in Supporting Information Table S1 are concentrations corresponding to the average blank signal plus three (LOD) and ten (LOQ) SDs of the blank . Method sensitivity was 10–100 times higher than the GC–MS methods used for the protein binders identification and it was also superior to the LC–MS method described in our previous study where C18 column was used . LOD and LOQ values listed in Supporting Information Table S1 were comparable with the sensitivity of similar method, where a ZIC–HILIC column was used .…”

“…AAs were quantified in the SRM mode (Supporting Information Fig. S2) as described in our previous study . Method LOD and LOQ values (the lowest amount of AA detectable in the sample) listed in Supporting Information Table S1 are concentrations corresponding to the average blank signal plus three (LOD) and ten (LOQ) SDs of the blank .…”

An electrochemical paper-based analytical sensor for one-step latex protein detection

“…BSA was used as the model NRL proteins. [33][34][35][36] Protein detection was conducted using the ePAD without an ex situ sample pretreatment step. A protein sample was reacted with the optimum 600 μg mL −1 Cu(II) at the reaction chamber for 1 min before the remaining Cu(II) was electrochemically measured.…”