Analysis of Ketoprofen and Mefenamic Acid by High- Performance Liquid Chromatography with Molecularly Imprinted Polymer as the Stationary Phase

Abstract

A simple and sensitive high-performance liquid chromatographic method for simultaneous determination of ketoprofen and mefenamic acid in tablets has been developed. HPLC with UV detection (220 nm) was performed on an analytical column packed with molecularly imprinted polymer (MIP) as the stationary phase. The MIPs are prepared by bulk polymerisation followed by crushing and sieving to the desired particle size. In this paper, we selected ketoprofen, methacrylic acid, and ethylene glycoldimethacrylate as template, functional monomer, and crosslinker in the presence of chloroform as the solvent. The retention times of mefenamic acid and ketoprofen were approximately 5 and 20 min, respectively. In order to compare the chromatographic data from the stationary phase, separation factors (alpha) were given. The values of alpha were 4.36 approximately 4.39 and showed that the MIPs were able to recognize structurally subtle differences from the template molecule. The limits of detection for ketoprofen and mefenamic acid were found to be 0.029 and 0.038 (g/L), while the limits of quantitation were 0.097 and 0.127 (g/L), respectively. Our results showed good accuracy, indicating that a ketoprofen-selective polymer was suitable for ketoprofen and mefenamic acid separations. Therefore, the MIPs are certainly applied to commercial tablet analysis.

Production of dry-state ketoprofen-encapsulated PMMA NPs by coupling micromixer-assisted nanoprecipitation and spray drying

“…Ketoprofen quantification was performed by UV spectrophotometry with a Schimadzu UV-2401 PC spectrophotometer (Kyoto, Japan) at 260 nm (see Figure S2 in supporting information). A previous study has indeed shown that, given the extremely low values of the Ketoprofen limits of quantification (LOQ, 0.122 µg/mL) and detection (LOD, 0.244 µg/mL) with a similar spectrophotometer as the one used in this study (Zafar et al, 2013), this method is appropriate to measure the concentration of Ketoprofen in the release medium (Hung and Hwang, 2008). A calibration curve was first obtained by plotting different known concentrations of Ketoprofen as a function of the peak intensity at 260 nm (see Figure S3 in supporting information).…”

Molecular imprinting science and technology: a survey of the literature for the years 2004-2011

“…Efforts to employ MIPs in assays have extended well beyond the large volume of literature focused upon MIPSE, most notably CE and CEC, and LC . In contrast to the 1990s and early 2000s, the proportion of studies devoted to, or heavily dependent upon, LC has reduced significantly.…”

Mefenamic Acid Selective Membranes Sensor and Its Application to pharmaceutical Analysis

“…Various methods have been reported for the determination of mefenamic acid as pure and in dosages forms. These methods include titrimetric [9,10], chromatographic [11][12], luminescence [13], flow injection [14][15], electrometric [16], spectroflourometric [17][18] and spectrophotometric methods [19][20][21][22].…”