Analysis of Kalmusia variispora associated with grapevine decline in Iran

Abed-Ashtiani, Farnaz; Narmani, Abolfazl; Arzanlou, Mahdi

doi:10.1007/s10658-019-01702-5

Cited by 18 publications

(14 citation statements)

References 24 publications

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“…Culture-dependent approaches and low-resolution molecular tools (i.e. Single-Strand Conformation Polymorphism) failed to unravel the full diversity of the endophytic fungal microbiome in vines [ 3 , 20 , 21 ]. This is due to the limited cultivability of most environmental microorganisms [ 22 ] and the bias of culture-dependent methods towards fast growing strains [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Grapevine wood microbiome analysis identifies key fungal pathogens and potential interactions with the bacterial community implicated in grapevine trunk disease appearance

Bekris

Vasileiadis

Elena

et al. 2021

Environmental Microbiome

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Background Grapevine trunk diseases (GTDs) is a disease complex caused by wood pathogenic fungi belonging to genera like Phaeomoniella, Phaeoacremonium, Fomitiporia, Eutypa and members of the family Botryosphaeriaceae. However, the co-occurrence of these fungi in symptomatic and asymptomatic vines at equivalent abundances has questioned their role in GTDs. Hence, we still lack a good understanding of the fungi involved in GTDs, their interactions and the factors controlling their assemblage in vines. We determined the fungal and bacterial microbiome in wood tissues of asymptomatic and symptomatic vines of three main Greek cultivars (Agiorgitiko, Xinomavro, Vidiano), each cultivated in geographically distinct viticultural zones, using amplicon sequencing. Results We noted that cultivar/biogeography (lumped factor) was the strongest determinant of the wood fungal microbiome (p < 0.001, 22.7%), while GTD symptoms condition had a weaker but still significant effect (p < 0.001, 3.5%), being prominent only in the cultivar Xinomavro. Several fungal Amplicon Sequence Variants (ASVs), reported as GTD-associated pathogens like Kalmusia variispora, Fomitiporia spp., and Phaemoniella chlamydosporα (most dominant in our study), were positively correlated with symptomatic vines in a cultivar/viticultural zone dependent manner. Random Forest analysis pointed to P. chlamydosporα, K. variispora, A. alternata and Cladosporium sp., as highly accurate predictors of symptomatic vines (0% error rate). The wood bacterial microbiome showed similar patterns, with biogeography/cultivar being the main determinant (p < 0.001, 25.5%) of its composition, followed by the GTD status of vines (p < 0.001, 5.2%). Differential abundance analysis revealed a universal positive correlation (p < 0.001) of Bacillus and Streptomyces ASVs with asymptomatic vines. Network analysis identified a significant negative co-occurrence network between these bacterial genera and Phaemoniella, Phaeoacrominum and Seimatosporium. These results point to a plant beneficial interaction between Bacillus/Streptomyces and GTD pathogens. Conclusions Our study (a) provides evidence that GTD symptomatic plants support a wood fungal microbiome, showing cultivar and biogeography-dependent patterns, that could be used as a proxy to distinguish between healthy and diseased vines, (b) points to strong interactions between the bacterial and fungal wood microbiome in asymptomatic vines that should be further pursued in the quest for discovery of novel biocontrol agents.

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Section: Introductionmentioning

confidence: 99%

Grapevine wood microbiome analysis identifies key fungal pathogens and potential interactions with the bacterial community implicated in grapevine trunk disease appearance

Bekris

Vasileiadis

Elena

et al. 2021

Environmental Microbiome

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“…Pestalotia-like fungi are among various fungi reported from grapevines showing trunk diseases symptoms in some countries (Larignon and Dubos 1997;Mugnai et al 1999;Graniti et al 2000;Mostert et al 2006;Steel et al 2007;Gramaje et al 2009;Úrbez-Torres et al 2009;Úrbez-Torres et al 2013;Mohammadi et al 2013;Úrbez-Torres et al 2014;Maharachchikumbura et al 2016;Pintos et al 2018;Abed-Ashtiani et al 2019;Cimmino et al 2020). Pestalotioid fungi are found as saprobes, endophytes and plant pathogens in association with mainly woody plants and human pathogens in different climates worldwide (De Hoog et al 2000;Watanabe et al 2010;Tanaka et al 2011;Liu et al 2019).…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Fungi associated with grapevine trunk diseases (GTDs) with emphasize on pestalotioid species in Kurdistan Province, Iran

Khaledi¹,

Moghadam²,

Abdollahzadeh³

et al. 2021

Preprint

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Grapevine trunk diseases (GTDs) are destructive and important economically with worldwide distribution. In this survey 233 fungal isolates were obtained from grapevine cultivars showing trunk diseases symptoms in Kurdistan Province, Iran. Based on sequences data and morphology 24 species belong to 20 genera were characterized. Botryosphaeriaceae, Alternaria, Sporocadaceae and Phaeoacremonium members were the most prevalent identified fungal groups. At the species level Botryosphaeria dothidea, Alternaria malorum, Phaeoacremonium aleophilum and Acremonium sclerotigenum were the most frequent identified species. All species are new records in Kurdistan Province. Clonostachys rosea and Neoscytalidium novaehollandiae are new records on grapevine in Iran. Acremonium sclerotigenum, Alternaria chlamydosporigena, Ascochyta herbicola and Paecilomyces formosus are new records on grapevine around the world. In phylogenetic analyses based on LSU, ITS, TEF-1α and TUB2 sequence data four pestalotioid species belong to Sporocadaceae were identified. Of these, three species are new for science and introduced here as Seimatosporium marivanicum, Sporocadus kurdistani and Xenoseimatosporium kurdistanicum. Furthermore, three new combinations in Sporocadus are proposed.

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“…Given that B. rosacearum has recently been introduced very little is known on the extent of distribution, host range and mechanisms of dispersal of this fungus in Zagros region and other part of the country. Since most of Biscogniauxia species has been reported on oak trees in various part of the world 15 and B. mediterranea the close species to B. rosacearum has reported as a serious threat for oak trees in Zagros region 22 , 23 , it is important to consider close cultivation of grapevine to oak forests in Zagros region that may have inherent risks in terms of the availability and dispersal of viable inoculum between vineyards and oak forests. Thus, it is a necessity to focus on pathogenicity of this species on different grapevine cultivars and fruit and forest trees especially oak species in Zagros forests, population biology of this fungal pathogen including epidemiology and population genetics, and integrated disease management consisting a combination of strategies and tactics including biological agents, sanitation, resistant genetic resources, pruning, etc.…”

Section: Discussionmentioning

confidence: 99%

Biscogniauxia rosacearum the charcoal canker agent as a pathogen associated with grapevine trunk diseases in Zagros region of Iran

Bahmani

Abdollahzadeh

Amini

et al. 2021

Sci Rep

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Grapevine trunk diseases (GTDs) are well-known and significant fungal diseases of Vitis vinifera with a worldwide distribution. During August to November 2016 in a survey to characterize fungi associated with grapevine trunk diseases in Kermanshah Province (west of Iran) vineyards, 286 fungal isolates were obtained. Based on morphology and DNA sequences data eight species were identified, of which Biscogniauxia rosacearum, Neoscytalidium hyalinum and Phaeoacremonium minimum were the most aggressive fungal pathogenic species characterized in this research. N. hyalinum was the most prevalent species. N. hyalinum and Ph. minimum have previously been reported from Vitis vinifera. Thus far, there are two records of Biscogniauxia mediterranea and Biscogniauxia capnodes on grapevine in the world with no data on pathology aspects. To our knowledge, it is the first time B. rosacearum is reported from grapevine across the globe. Pathogenicity test with three strains of B. rosacearum on 2-year-old potted grapevines confirmed the pathogenicity of B. rosacearum on grapevine. The proximity of vineyards to the oak trees in Zagros forests as one of the plant hosts of Biscogniauxia spp. further highlights the need for extensive studies on B. rosacearum as a new fungal pathogen.

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Analysis of Kalmusia variispora associated with grapevine decline in Iran

Cited by 18 publications

References 24 publications

Grapevine wood microbiome analysis identifies key fungal pathogens and potential interactions with the bacterial community implicated in grapevine trunk disease appearance

Grapevine wood microbiome analysis identifies key fungal pathogens and potential interactions with the bacterial community implicated in grapevine trunk disease appearance

WITHDRAWN: Fungi associated with grapevine trunk diseases (GTDs) with emphasize on pestalotioid species in Kurdistan Province, Iran

Biscogniauxia rosacearum the charcoal canker agent as a pathogen associated with grapevine trunk diseases in Zagros region of Iran

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