Analysis of isoquinoline alkaloids using chitosan‐assisted liquid–solid extraction followed by microemulsion liquid chromatography employing a sub‐2‐micron particle stationary phase

Abstract

A simple, efficient, and green chitosan-assisted liquid-solid extraction method was developed for the sample preparation of isoquinoline derivative alkaloids followed by microemulsion LC. The optimized mobile phase consisted of 0.8% w/v of ethyl acetate, 1.0% w/v of SDS, 8.0% w/v of n-butanol, 0.1% v/v acetic acid, and 10% v/v ACN. Compared to pharmacopoeia method and organic solvent extraction, this new approach avoided the use of volatile organic solvents, replacing them with relatively small amounts of chitosan. Under the optimum conditions, good linearity (r > 0.9980) for all calibration curves and low detection limits between 0.05 and 0.10 μg/mL were achieved. The presented procedure was successfully applied to determine alkaloids in Rhizoma coptidis with satisfactory recoveries (81.3-106.4%).

Modification and Characterization of Fe3O4 Nanoparticles for Use in Adsorption of Alkaloids

“…The mixture was dried by nitrogen blower at 80 °C. The residue was redissolved in 400 μL of methanol, which was filtered (0.22 μm) for subsequent HPLC analysis [ 19 , 20 , 21 ].…”

Functionalization of cellulose as imprinted adsorbent for selective adsorption of matrine

“…Some techniques have been successfully applied to separate the alkaloids including liquid–liquid extraction, liquid–solid extraction, ion‐exchange, adsorption, and chromatography methods, such as micellar liquid chormatography (MLC) and high‐performance liquid chromatography . Among those methods, adsorption is considered as one of the simplest and most effective process.…”