Analysis of isolation of cerebral cortical neurons in rats by different
methods

Li, Jianhua; Zhang, Yuegang; Zhang, Tao; Tian, Mingxing; Hou, Jing; Huang, Dengliang; Cheng, Yan Zhong; Zhu, Man; Su, Xiaoming; Li, Zhiqin; Tong, Sixian; Zhang, Xuan; Deng, Jun; Dong, Yuemei; Ma, Yue

doi:10.32604/biocell.2020.08941

Cited by 3 publications

(1 citation statement)

References 19 publications

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“…Hippocampus cells were isolated from C57BL/6J mice, and after 6 days of culture hippocampus cells were validated by staining with neuron marker NeuN, following standard protocols. 16 Cell were incubated with 5.5 mM glucose in normal medium (control group) or 75 mM glucose (HG group).…”

Section: Methodsmentioning

confidence: 99%

MALAT1 Regulated mTOR-Mediated Tau Hyperphosphorylation by Acting as a ceRNA of miR144 in Hippocampus Cells Exposed to High Glucose

Lu¹,

Zhao²,

Cao³

et al. 2021

CIA

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Aim High glucose (HG)-induced activation of mTOR promotes tau phosphorylation and leads to diabetes-associated dementia. This study aimed to explore the role of metastasis associated in lung adenocarcinoma transcript 1 (MALAT1) in HG-induced neuronal cell injury. Methods Hippocampus cells were isolated from C57BL/6J mice. After 6 days of culture, the cells were incubated with 5.5 mM glucose in normal medium or 75 mM glucose for 4 days. Cells were transfected with miR-144 mimic, miR-144 inhibitor, siRNA for MALAT1 or corresponding controls. Gene expression was detected by PCR and Western blot analysis. Results HG increased the levels of MALAT1 and p-tau in hippocampal cells. Knockdown of MALAT1 partially reversed the effects of HG on mTOR activity and p-tau protein levels. MALAT1 functioned as competing endogenous RNA (ceRNA) for miR-144, and pre-treatment with MALAT1 siRNA decreased mTOR activity and p-tau protein level in HG-treated hippocampal cells, which was significantly attenuated by miR-144 mimics. Moreover, miR-144 negatively regulated the expression of mTOR and knockdown of MALAT1 suppressed mTOR, while overexpression of mTOR abrogated protective effects of MALAT1 knockdown in HG-treated hippocampal cells. Conclusion MALAT1 knockdown prevented HG-induced mTOR activation and inhibited tau phosphorylation. MALAT1 may be a therapy target for diabetes associated dementia.

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Section: Methodsmentioning

confidence: 99%

MALAT1 Regulated mTOR-Mediated Tau Hyperphosphorylation by Acting as a ceRNA of miR144 in Hippocampus Cells Exposed to High Glucose

Lu¹,

Zhao²,

Cao³

et al. 2021

CIA

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Review of technology and materials for the development of cultured meat

Lee

Jeong

et al. 2022

Critical Reviews in Food Science and Nutrition

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Association of Essential Tremor With Dementia and Affective Disorders: A Meta-Analysis

Shang

Chen

et al. 2022

Front. Neurol.

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BackgroundThe dementia and affective disorders are common non-motor features in patients with essential tremor (ET). However, the relationship of ET with cognitive impairments and affective disorders remains controversial. This meta-analysis aimed to analyze the association of ET with dementia and affective disorders.MethodsOriginal studies published from January 1999 to October 2019 were systematically searched from the database of Medline (OvidSP), EMBASE (OvidSP), and the Cochrane Central Register of Controlled Trials. Pooled standard mean difference (SMD, random effect model), odds ratios (ORs), relative risk (RR), and 95% CI were calculated.ResultsCompared with the Non-ET group, patients with ET had significantly lower Mini-Mental State Examination (MMSE) score (SMD, −1.16; 95% CI, −1.75 to −0.58; p = 0.0001) and had significantly higher depressive and anxiety symptoms scale score (SMD, 0.55; 95% CI, 0.22–0.87; p = 0.0009). The OR for dementia and affective disorders in individuals with ET compared with individuals without ET was 2.49 (95% CI, 2.17–2.85, p < 0.00001). While there was no significant difference in Montreal Cognitive Assessment (MoCA) score between ET and Non-ET groups (SMD, −0.52; 95% CI, −0.16 to 0.13; p = 0.23), there was a significant difference in the risk of mortality between ET and Non-ET groups (RR = 4.69, 95% CI, 2.18–10.07).ConclusionThe non-motor symptoms should not be neglected among patients with ET. However, the causal relationship between ET and dementia, depression, and anxiety is unclear.

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Analysis of isolation of cerebral cortical neurons in rats by different methods

Cited by 3 publications

References 19 publications

MALAT1 Regulated mTOR-Mediated Tau Hyperphosphorylation by Acting as a ceRNA of miR144 in Hippocampus Cells Exposed to High Glucose

MALAT1 Regulated mTOR-Mediated Tau Hyperphosphorylation by Acting as a ceRNA of miR144 in Hippocampus Cells Exposed to High Glucose

Review of technology and materials for the development of cultured meat

Association of Essential Tremor With Dementia and Affective Disorders: A Meta-Analysis

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