Analysis of Iron in Ferritin, the Iron-Storage Protein: A General Chemistry Experiment

Donlin, Maureen J.; Frey, Regina F.; Putnam, Christopher D.; Proctor, Jody; Bashkin, James K.

doi:10.1021/ed075p437

Cited by 22 publications

(18 citation statements)

References 10 publications

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“…Many reducing agents and chelators can be used to trigger iron release, as presented in several studies [15,17,18]. In the present study, light or ionizing radiation and ferrozine were chosen as the reducing agent and the chelator, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…The ferrous ion, Fe(II), becomes highly absorbing to visible light when it forms a complex with the organic chelator, ferrozine. This very stable complex, Fe ferrozine ð Þ 4À 3 , is dark purple and can be spectrophotometrically detected at the wavelength of 562 nm, even at a very low concentration, due to the high extinction coefficient of 27,900 dm 3 mol -1 [17]. In photochemical studies, we illuminated the sample of ferritin (0.1 mg/mL) with laser light, wavelength 308 nm, in anaerobic conditions.…”

Section: Resultsmentioning

confidence: 99%

“…The optical path of the quartz cell was 0.1 or 0.02 cm. The calculations to determine the total amount of iron per molecule of ferritin were done according to [17].…”

Section: Methodsmentioning

confidence: 99%

“…The six fourfold channels are very hydrophobic, whereas the eight threefold channels are lined with polar groups and are, therefore, hydrophilic. Iron is probably transported through the threefold channels [2,[16][17][18], whereas the fourfold channel is assumed to transport other small molecules, such as molecular oxygen or reducing agents [2,16,17].…”

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confidence: 99%

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Iron release from ferritin induced by light and ionizing radiation

Wolszczak

Gajda²

2010

Res Chem Intermed

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The reductive release of iron from ferritin by UV light or ionizing radiation has been investigated in separate experiments. When ferritin is exposed to light, the mineral core is the main photoreceptor for the Fe(III) reduction. In radiolytic studies, we determined that, in the absence of oxygen, the hydrated electron (e aq -) is the reducing agent triggering redox reactions associated with iron mobilization from ferritin. In an aerobic system, the superoxide radical anion (O 2•-) is also involved in the iron release process. We found that, in photochemical and radiolytical studies, Fe(II) mobilization from ferritin required an iron chelator. Without a chelator, ferritin is an electron-storage molecule for a long period, on the order of at least several hours. The reductant or chelator entry into the ferritin core is not necessary for iron release. The ferrozine is a convenient chelating agent to monitor Fe(II) mobilization, due to a high extinction coefficient of Fe ferrozine ð Þ 4À 3and a high rate constant of complexation process (2.65 9 10 4 dm 3 mol -1 s -1 ).

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…The optical path of the quartz cell was 0.1 or 0.02 cm. The calculations to determine the total amount of iron per molecule of ferritin were done according to [17].…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Iron release from ferritin induced by light and ionizing radiation

Wolszczak

Gajda²

2010

Res Chem Intermed

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“…Excess levels of free iron, however, are considered to be cytotoxic and can cause severe neurological disorders, such as Parkinson's disease, Friedrich's ataxia, Alzheimer's disease and Pica [3][4][5][6]. Many excellent reagents and techniques have been developed for the detection of Fe 3+ and Fe 2+ [7][8][9][10][11]. Most of them, however, are only effective at neutral pH.…”

Section: Introductionmentioning

confidence: 99%