Analysis of intercellular communication in the osteosarcoma microenvironment based on single cell sequencing data

Chen, Fangyi; Liu, Jiao; Yang, Tao; Sun, Jianwei; He, Xianwei; Fu, Xinping; Qiao, Shigang; An, Jianzhong; Yang, Jun

doi:10.1016/j.jbo.2023.100493

Cited by 3 publications

(5 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With some heterogeneity across different OS tumors, malignant osteoblastic cells and myeloid cells are the most represented elements [30][31][32][33]. The variegated cellular microenvironment is reflected by a complex network of factors and receptors.…”

Section: Il-1 Family In Osteosarcomamentioning

confidence: 99%

“…The variegated cellular microenvironment is reflected by a complex network of factors and receptors. Inside OS tumors, multiple ligand-receptor interactions occur, also involving IL-1β [30,31]. An analysis of OS tumor infiltrates by scRNA-seq revealed that the majority of TAMs in OS exhibited M2 polarization [32], and they may support immune escape and metastatic progression by secreting various immunosuppressive and angiogenic factors, such as IL-1β, IL-4, IL-10, and TGF-β.…”

Section: Il-1 Family In Osteosarcomamentioning

confidence: 99%

“…Moreover, in response to doxorubicin, IL-1β signaling can upregulate the expression of PD-L1 in OS cells, favoring immune evasion [44]. A comparison of ligand-receptor interactions between naive and treated patients showed that chemotherapy can induce a strong remodeling of the TME, with a decrease in immune cell communication and an increase in interactions between malignant osteoblasts [30].…”

Section: Il-1 Family In Osteosarcomamentioning

confidence: 99%

See 2 more Smart Citations

IL-1 Family Members in Bone Sarcomas

Landuzzi,

Ruzzi,

Pellegrini

et al. 2024

Cells

View full text Add to dashboard Cite

IL-1 family members have multiple pleiotropic functions affecting various tissues and cells, including the regulation of the immune response, hematopoietic homeostasis, bone remodeling, neuronal physiology, and synaptic plasticity. Many of these activities are involved in various pathological processes and immunological disorders, including tumor initiation and progression. Indeed, IL-1 family members have been described to contribute to shaping the tumor microenvironment (TME), determining immune evasion and drug resistance, and to sustain tumor aggressiveness and metastasis. This review addresses the role of IL-1 family members in bone sarcomas, particularly the highly metastatic osteosarcoma (OS) and Ewing sarcoma (EWS), and discusses the IL-1-family-related mechanisms that play a role in bone metastasis development. We also consider the therapeutic implications of targeting IL-1 family members, which have been proposed as (i) relevant targets for anti-tumor and anti-metastatic drugs; (ii) immune checkpoints for immune suppression; and (iii) potential antigens for immunotherapy.

show abstract

Section: Il-1 Family In Osteosarcomamentioning

confidence: 99%

Section: Il-1 Family In Osteosarcomamentioning

confidence: 99%

Section: Il-1 Family In Osteosarcomamentioning

confidence: 99%

See 1 more Smart Citation

IL-1 Family Members in Bone Sarcomas

Landuzzi,

Ruzzi,

Pellegrini

et al. 2024

Cells

View full text Add to dashboard Cite

show abstract

“…Myeloid cells are the most abundant cell type in the TME of OS. A single-cell analysis showed multiple ligand-receptor interactions between OS tumor, myeloid, and osteoblast cells, including 21 ligand-receptor gene pairs that are significantly associated with survival outcomes [20]. The TME not only provides favorable conditions for tumor cell growth but also releases a range of elements, including various cytokines, chemokines, and growth factors, that can promote the metastasis of tumor cells to other tissues and organs [21].…”

Section: The Tumor Microenvironment Of Osmentioning

confidence: 99%

“…The OCs are specialized cells responsible for bone resorption that originate from myeloid precursor cells [20]. OCs communicate closely with osteoblasts and osteocytes under normal physiological conditions and play a vital role in maintaining bone homeostasis [29,30].…”

Section: Osteoclast Cellsmentioning

confidence: 99%

Decoding the Impact of Tumor Microenvironment in Osteosarcoma Progression and Metastasis

Nirala,

Yamamichi,

Petrescu

et al. 2023

Cancers

View full text Add to dashboard Cite

Osteosarcoma (OS) is a heterogeneous, highly metastatic bone malignancy in children and adolescents. Despite advancements in multimodal treatment strategies, the prognosis for patients with metastatic or recurrent disease has not improved significantly in the last four decades. OS is a highly heterogeneous tumor; its genetic background and the mechanism of oncogenesis are not well defined. Unfortunately, no effective molecular targeted therapy is currently available for this disease. Understanding osteosarcoma’s tumor microenvironment (TME) has recently gained much interest among scientists hoping to provide valuable insights into tumor heterogeneity, progression, metastasis, and the identification of novel therapeutic avenues. Here, we review the current understanding of the TME of OS, including different cellular and noncellular components, their crosstalk with OS tumor cells, and their involvement in tumor progression and metastasis. We also highlight past/current clinical trials targeting the TME of OS for effective therapies and potential future therapeutic strategies with negligible adverse effects.

show abstract

Exploration of the molecular mechanism of intercellular communication in paediatric neuroblastoma by single-cell sequencing

Chu

2023

Sci Rep

View full text Add to dashboard Cite

Neuroblastoma (NB) is an embryonic tumour that originates in the sympathetic nervous system and occurs most often in infants and children under 2 years of age. Moreover, it is the most common extracranial solid tumour in children. Increasing studies suggest that intercellular communication within the tumour microenvironment is closely related to tumour development. This study aimed to construct a prognosis-related intercellular communication-associated genes model by single-cell sequencing and transcriptome sequencing to predict the prognosis of patients with NB for precise management. Single-cell data from patients with NB were downloaded from the gene expression omnibus database for comprehensive analysis. Furthermore, prognosis-related genes were screened in the TARGET database based on epithelial cell marker genes through a combination of Cox regression and Lasso regression analyses, using GSE62564 and GSE85047 for external validation. The patients’ risk scores were calculated, followed by immune infiltration analysis, drug sensitivity analysis, and enrichment analysis of risk scores, which were conducted for the prognostic model. I used the Lasso regression feature selection algorithm to screen characteristic genes in NB and developed a 21-gene prognostic model. The risk scores were highly correlated with multiple immune cells and common anti-tumour drugs. Furthermore, the risk score was identified as an independent prognostic factor for NB. In this study, I constructed and validated a prognostic signature based on epithelial marker genes, which may provide useful information on the development and prognosis of NB.

show abstract

Analysis of intercellular communication in the osteosarcoma microenvironment based on single cell sequencing data

Cited by 3 publications

References 54 publications

IL-1 Family Members in Bone Sarcomas

IL-1 Family Members in Bone Sarcomas

Decoding the Impact of Tumor Microenvironment in Osteosarcoma Progression and Metastasis

Exploration of the molecular mechanism of intercellular communication in paediatric neuroblastoma by single-cell sequencing

Contact Info

Product

Resources

About