Analysis of interactions between the corneal epithelium and liposomes: Qualitative and quantitative fluorescence studies of a corneal epithelial cell line

Pleyer, Uwe; Grammer, Joachim B.; Kosmidis, Perikles; Rückert, D.

doi:10.1016/s0039-6257(05)80068-2

Cited by 18 publications

(15 citation statements)

References 30 publications

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“…It has been postulated that the DNA is directly delivered to the cytoplasm via a fusion process with the cell membrane (Felgner and Ringold, 1989;Litzinger and Huang, 1992). We have previously shown that liposomes are able to deliver a¯uorescent marker to the nucleii of corneal cells (Pleyer et al, 1995). There is evidence from these experiments which discounts delivery via fusion of liposomes with the cell membrane.…”

Section: Discussionmentioning

confidence: 94%

Efficiency and Toxicity of Liposome-mediated Gene Transfer to Corneal Endothelial Cells

Pleyer

Groth

Hinz

et al. 2001

Experimental Eye Research

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Section: Discussionmentioning

confidence: 94%

Efficiency and Toxicity of Liposome-mediated Gene Transfer to Corneal Endothelial Cells

Pleyer

Groth

Hinz

et al. 2001

Experimental Eye Research

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“…Although in experimental models, SIRCs are described and used as being of epithelial origin [26][27][28], their morphology, ultrastructure and antigenic properties indicate a fibroblast origin for this cell line [29].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant protection in cultured corneal cells and whole corneas submitted to UV-B exposure

Ciuffi

Pisanello

Pagliai

et al. 2003

Journal of Photochemistry and Photobiology B: Biology

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Several corneal pathologies are characterized by the presence of reactive oxygen species (ROS); therefore, we evaluated the protection afforded by pirenoxine and melatonin to corneal cell culture and whole rabbit cornea from ultraviolet exposure and other oxidant systems.Rabbit cornea cell (SIRC) plates and whole corneas were exposed to UV-B (80 or 800 mJ/cm 2 ) or incubated with fMLPstimulated autologous macrophages, in the presence or absence of pirenoxine or melatonin (10 À5 M). The protective activity of compounds was assessed by measuring superoxide anion formation, inhibition of oxidation and mitochondrial viability. Moreover the ex vivo protective effect of pirenoxine and melatonin was verified in the whole cornea submitted to UV-B exposure in vitro.Our experimental data demonstrate that pirenoxine and melatonin were able to inhibit the superoxide formation and oxidative effect in cell culture and whole rabbit corneas submitted to UV-B exposure or to incubation with fMLP-stimulated autologous macrophages.Mitochondrial viability was restored in epithelial cells of rabbit cornea but not in SIRCs. Moreover, both compounds are also able to increase ex vivo epithelial corneal cell defences against the in vitro UV-B induced lipid peroxidation.

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“…The SIRC cell line was originally derived from the cornea of a normal hare 24 and has been widely used as an in vitro model to assess corneal transport as well as immunological and toxicological responses. [25][26][27][28][29][30][31] However, contradictory reports exist about the epithelial origin of the cell line. Some investigators refer to the cell line as fibroblastic, 24 whereas others claim that it is of epithelial origin.…”

Section: Introductionmentioning

confidence: 99%

Functional differences in nucleoside and nucleobase transporters expressed on the rabbit corneal epithelial cell line (SIRC) and isolated rabbit cornea

et al. 2003

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ABSTRACTclovir permeates the cornea primarily by passive diffusion. Nucleoside and nucleobase transporters on rabbit cornea and corneal epithelial cell line, SIRC, are functionally different, undermining the utility of the SIRC cell line as an in vitro screening tool for elucidating the corneal permeation mechanism of nucleoside analogs. Uptake and transport studies were performed for 2 minutes and 120 minutes, respectively, at 34°C. Thymidine uptake by SIRC displayed saturable kinetics (K m = 595.9 ± 80.4μM, and V max = 289.5 ± 17.2 pmol/min/mg protein). Uptake was inhibited by both purine and pyrimidine nucleosides but not by nucleobases. [ 3 H]thymidine uptake was sodium and energy independent but was inhibited by nitrobenzylthioinosine at nanomolar concentrations. Adenine uptake by SIRC consisted of a saturable component (K m = 14.4 ± 2.3μM, V max = 0.4 ± 0.04 nmol/min/mg protein) and a nonsaturable component. Uptake of adenine was inhibited by purine nucleobases but not by the nucleosides or pyrimidine nucleobases and was independent of sodium, energy, and nitrobenzylthioinosine. [ 3 H]ganciclovir uptake involved a carrier-mediated component and was inhibited by the purine nucleobases but not by the nucleosides or pyrimidine nucleobases. However, transport of [ 3 H]adenine across the isolated rabbit cornea was not inhibited by unlabeled adenine. Further, corneal permeability of ganciclovir across a 100-fold concentration range remained constant, indicating that ganci-

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Analysis of interactions between the corneal epithelium and liposomes: Qualitative and quantitative fluorescence studies of a corneal epithelial cell line

Cited by 18 publications

References 30 publications

Efficiency and Toxicity of Liposome-mediated Gene Transfer to Corneal Endothelial Cells

Efficiency and Toxicity of Liposome-mediated Gene Transfer to Corneal Endothelial Cells

Antioxidant protection in cultured corneal cells and whole corneas submitted to UV-B exposure

Functional differences in nucleoside and nucleobase transporters expressed on the rabbit corneal epithelial cell line (SIRC) and isolated rabbit cornea

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