Analysis of interactions among the CLAVATA3 receptors reveals a direct interaction between CLAVATA2 and CORYNE in Arabidopsis

Zhu, Yingfang; Wang, Yuqing; Li, Ruili; Song, Xiu‐Fen; Wang, Qinli; Huang, Shanjin; Jin, Jing Bo; Liu, Chunming; Lin, Jinxing

doi:10.1111/j.1365-313x.2009.04049.x

Cited by 120 publications

(140 citation statements)

References 36 publications

(45 reference statements)

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“…Since multiple RLKs are known to form homodimers (Russinova et al, 2004;Hink et al, 2008;Zhu et al, 2010), we first tested for an interaction of CST with itself. CST was found to self-interact at the plasma membrane, as cotransfection of CST-YFPn/CST-YFPc resulted in a similar pattern of fluorescence as CST-GFP (Figs.…”

Section: Cst Interacts With the Hae And Evr Rlks At The Plasma Membranementioning

confidence: 99%

CAST AWAY, a Membrane-Associated Receptor-Like Kinase, Inhibits Organ Abscission in Arabidopsis

et al. 2011

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Receptor-like kinase-mediated cell signaling pathways play fundamental roles in many aspects of plant growth and development. A pair of Arabidopsis (Arabidopsis thaliana) leucine-rich repeat receptor-like kinases (LRR-RLKs), HAESA (HAE) and HAESA-LIKE2 (HSL2), have been shown to activate the cell separation process that leads to organ abscission. Another pair of LRR-RLKs, EVERSHED (EVR) and SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1, act as inhibitors of abscission, potentially by modulating HAE/HSL2 activity. Cycling of these RLKs to and from the cell surface may be regulated by NEVERSHED (NEV), a membrane trafficking regulator that is essential for organ abscission. We report here the characterization of CAST AWAY (CST), a receptor-like cytoplasmic kinase that acts as a spatial inhibitor of cell separation. Disruption of CST suppresses the abscission defects of nev mutant flowers and restores the discrete identity of the trans-Golgi network in nev abscission zones. After organ shedding, enlarged abscission zones with obscured boundaries are found in nev cst flowers. We show that CST is a dual-specificity kinase in vitro and that myristoylation at its amino terminus promotes association with the plasma membrane. Using the bimolecular fluorescence complementation assay, we have detected interactions of CST with HAE and EVR at the plasma membrane of Arabidopsis protoplasts and hypothesize that CST negatively regulates cell separation signaling directly and indirectly. A model integrating the potential roles of receptor-like kinase signaling and membrane trafficking during organ separation is presented.

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Section: Cst Interacts With the Hae And Evr Rlks At The Plasma Membranementioning

confidence: 99%

CAST AWAY, a Membrane-Associated Receptor-Like Kinase, Inhibits Organ Abscission in Arabidopsis

et al. 2011

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“…For example, CLV3 and CLE40 are responsible for shoot and root apical meristem maintenance, respectively [9,11,12]. CLV3 is perceived by three different receptors or putative receptors, CLAVA-TA1 (CLV1), CLAVATA 2 (CLV2)-CORYNE (CRN), and RPK2/TOAD2, to limit stem cell size by restricting the expression of a homeodomain transcription factor, WUSCHEL (WUS), via a negative feedback loop [13][14][15][16][17][18]. On the other hand, CLE40 is perceived by its putative receptor complex containing two different RLKs, ARA-BIDOPSIS CRIKLY4 (ACR4) and CLV1, to inhibit the expression of a root version of homeodomain transcription factor, WOX5 [19,20].…”

Section: Introductionmentioning

confidence: 99%

RGF1 INSENSITIVE 1 to 5, a group of LRR receptor-like kinases, are essential for the perception of root meristem growth factor 1 in Arabidopsis thaliana

et al. 2016

Cell Res

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“…8,13 However, the ligand-receptor interaction between CLV3 peptide and LRR of CLV2 should be verified in future. In addition, the third pathway consists of CLV1 and CLV2-CRN heterodimers.…”

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confidence: 99%

Multiple receptor complexes assembled for transmitting CLV3 signaling in Arabidopsis

Zhu

Wan

Lin

2010

Plant Signaling & Behavior

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In Arabidopsis, the feedback regulatory loop between CLAVATA3 (CLV3) signaling pathway and transcription factor, WUSCHEL (WUS) plays a significant role in shoot apical meristems (SAM) maintenance. Previously, CLV1/CLV2 heterodimers were supposed to perceive and transmit CLV3 signaling. Recent genetic analysis isolated a novel receptor kinase, CORYNE (CRN), which was found to be involved in the CLV3 pathway. Therefore, new hypothesis was put forward that CRN probably acts with CLV2 to transmit CLV3 in parallel with CLV1 based on genetic analysis. In our recent work, we took advantage of firefly luciferase complementation imaging (LCI) assay to analyze the interactions among CLV1, CLV2 and CRN in both Arabidopsis thaliana protoplasts and Nicotiana benthamiana leaves. We identified the physical interaction between CLV2 and CRN in the absence of CLV3 and found some interesting phenomenon such as CLV1, CLV2 and CRN may form a complex, and that CRN was able to form homodimers. These new observations make the relationships among these three proteins more complex than that indicated in two-parallel pathway model. Combining current genetic and our new biochemical evidence, a more possible and detailed model for CLV3 pathway was developed.The stem cells located in SAM constantly progress cell division and cell differentiation to generate all of the aboveground organs in plants. CLV3 signaling pathway negatively regulates the fate of stem cells and maintains the SAM size through repressing the homeodomain transcription factor, WUS, which promotes the stem cell differentiation.1-3 Current knowledge believes that CLV3 encodes a secreted extracellular peptide, 4 and CLV1 encodes a leucine-rich-repeat receptor-like kinase (LRR-RLK); CLV2 encodes a receptor-like protein (RLP) lacking a kinase domain. 5,6 Previous studies hypothesized that CLV1/ CLV2 heterodimers function as receptors to transmit CLV3 ligand signaling. 4,7 Recently, a new gene, CRN/SOL2, was isolated and predicted to encode membrane-associated kinase with a very short extracellular domain. Genetic evidences predicted that SOL2/CRN functions together with CLV2 in parallel with CLV1 in CLV3 signaling. 8,9 Accordingly new two-parallel pathway model was raised that: one uses CLV1 homodimers and the other uses CRN-CLV2 heterodimers. 8 In our recent work, we seek to analyze the interactions among these receptor proteins at biochemical level.Owing to the specific and limited expression patterns of these receptor proteins, it would be difficult to conduct biochemical analysis only using SAM due to its slight amount. Therefore, we took advantage of transient expression systems including Arabidopsis protoplasts and N. benthamiana leaves to analyze the interactions among CLV1, CLV2 and CRN using the newly developed LCI method, which is believed to have high signal-tonoise ratio and be able to detect dynamic interactions including membrane protein interactions.

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Analysis of interactions among the CLAVATA3 receptors reveals a direct interaction between CLAVATA2 and CORYNE in Arabidopsis

Cited by 120 publications

References 36 publications

CAST AWAY, a Membrane-Associated Receptor-Like Kinase, Inhibits Organ Abscission in Arabidopsis

CAST AWAY, a Membrane-Associated Receptor-Like Kinase, Inhibits Organ Abscission in Arabidopsis

RGF1 INSENSITIVE 1 to 5, a group of LRR receptor-like kinases, are essential for the perception of root meristem growth factor 1 in Arabidopsis thaliana

Multiple receptor complexes assembled for transmitting CLV3 signaling in Arabidopsis

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