Analysis of inducers of xylanase and cellulase activities production by Ganoderma applanatum LPB MR-56

Salmon, Denise Naomi Xavier; Spier, Michele Rigon; Soccol, Carlos Ricardo; Vandenberghe, Luciana Porto de Souza; Montibeller, Valesca Weingartner; Bier, Mário César Jucoski; Faraco, Vincenza

doi:10.1016/j.funbio.2014.04.003

Cited by 27 publications

(14 citation statements)

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“…Among the three substrates tested, crystalline cellulose was found to be the best carbon source providing the highest cellulase and xylanase activity of all fungi ( Table 1 ). This finding is in agreement with several reports showing cellulases and xylanases production by Basidiomycota species in the presence of several potential inducers [ 15 , 16 , 17 ].…”

Section: Resultssupporting

confidence: 94%

The Carbon Source Controls the Secretion and Yield of Polysaccharide-Hydrolyzing Enzymes of Basidiomycetes

2021

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In the present study, the polysaccharide-hydrolyzing secretomes of Irpex lacteus (Fr.) Fr. (1828) BCC104, Pycnoporus coccineus (Fr.) Bondartsev and Singer (1941) BCC310, and Schizophyllum commune Fr. (1815) BCC632 were analyzed in submerged fermentation conditions to elucidate the effect of chemically and structurally different carbon sources on the expression of cellulases and xylanase. Among polymeric substrates, crystalline cellulose appeared to be the best carbon source providing the highest endoglucanase, total cellulase, and xylanase activities. Mandarin pomace as a growth substrate for S. commune allowed to achieve comparatively high volumetric activities of all target enzymes while wheat straw induced a significant secretion of cellulase and xylanase activities of I. lacteus and P. coccineus. An additive effect on the secretion of cellulases and xylanases by the tested fungi was observed when crystalline cellulose was combined with mandarin pomace. In I. lacteus the cellulase and xylanase production is inducible in the presence of cellulose-rich substrates but is suppressed in the presence of an excess of easily metabolizable carbon source. These enzymes are expressed in a coordinated manner under all conditions studied. It was shown that the substitution of glucose in the inoculum medium with Avicel provides accelerated enzyme production by I. lacteus and higher cellulase and xylanase activities of the fungus. These results add new knowledge to the physiology of basidiomycetes to improve cellulase production.

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Section: Resultssupporting

confidence: 94%

The Carbon Source Controls the Secretion and Yield of Polysaccharide-Hydrolyzing Enzymes of Basidiomycetes

2021

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“…Although all yeasts were able to grow in medium with SS or CMC as the sole carbon source, the corresponding extracellular enzyme activity was not observed in some of the protein samples as mentioned above. Considering that it has been previously reported that CMC has an inducing effect in the cellulase activity from the fungus Ganoderma applanatum MR-56 [ 27 ] and that amylolytic activity was induced by SS in bacterial isolates [ 28 ], it is possible that the yeasts tested in this work require the presence of the substrate to induce the expression of the corresponding genes that encode the respective enzyme activity. To evaluate this possibility, the yeasts were cultured in YM medium supplemented with SS or CMC, the extracellular proteins were obtained and the activities measured (Additional file 1 ).…”

Section: Resultsmentioning

confidence: 99%

Screening and characterization of amylase and cellulase activities in psychrotolerant yeasts

et al. 2016

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BackgroundAmylases and cellulases have great potential for application in industries such as food, detergent, laundry, textile, baking and biofuels. A common requirement in these fields is to reduce the temperatures of the processes, leading to a continuous search for microorganisms that secrete cold-active amylases and cellulases. Psychrotolerant yeasts are good candidates because they inhabit cold-environments. In this work, we analyzed the ability of yeasts isolated from the Antarctic region to grow on starch or carboxymethylcellulose, and their potential extracellular amylases and cellulases.ResultAll tested yeasts were able to grow with soluble starch or carboxymethylcellulose as the sole carbon source; however, not all of them produced ethanol by fermentation of these carbon sources. For the majority of the yeast species, the extracellular amylase or cellulase activity was higher when cultured in medium supplemented with glucose rather than with soluble starch or carboxymethylcellulose. Additionally, higher amylase activities were observed when tested at pH 5.4 and 6.2, and at 30–37 °C, except for Rhodotorula glacialis that showed elevated activity at 10–22 °C. In general, cellulase activity was high until pH 6.2 and between 22–37 °C, while the sample from Mrakia blollopis showed high activity at 4–22 °C. Peptide mass fingerprinting analysis of a potential amylase from Tetracladium sp. of about 70 kDa, showed several peptides with positive matches with glucoamylases from other fungi.ConclusionsAlmost all yeast species showed extracellular amylase or cellulase activity, and an inducing effect by the respective substrate was observed in a minor number of yeasts. These enzymatic activities were higher at 30 °C in most yeast, with highest amylase and cellulase activity in Tetracladium sp. and M. gelida, respectively. However, Rh. glacialis and M. blollopis displayed high amylase or cellulase activity, respectively, under 22 °C. In this sense, these yeasts are interesting candidates for industrial processes that require lower temperatures.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-016-0640-8) contains supplementary material, which is available to authorized users.

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“…Several efforts are under way to improve both the molecular systems, hydrolytic enzymes (Amore et al 2012 , 2013 , 2015 ; Salmon et al 2014 ; Weingartner Montibeller et al 2014 ; Giacobbe et al 2014 ), and microorganisms (Liguori et al 2015 ; Ventorino et al 2015 ), and bioreactor systems adopted for the biomasses bioconversion process in order to solve the environmental and economical issues of the process (Wang et al 2011 ; Khoo 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Bioreactors for lignocellulose conversion into fermentable sugars for production of high added value products

Liguori

Ventorino

Pepe

et al. 2015

Appl Microbiol Biotechnol

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Lignocellulosic biomasses derived from dedicated crops and agro-industrial residual materials are promising renewable resources for the production of fuels and other added value bioproducts. Due to the tolerance to a wide range of environments, the dedicated crops can be cultivated on marginal lands, avoiding conflict with food production and having beneficial effects on the environment. Besides, the agro-industrial residual materials represent an abundant, available, and cheap source of bioproducts that completely cut out the economical and environmental issues related to the cultivation of energy crops. Different processing steps like pretreatment, hydrolysis and microbial fermentation are needed to convert biomass into added value bioproducts. The reactor configuration, the operative conditions, and the operation mode of the conversion processes are crucial parameters for a high yield and productivity of the biomass bioconversion process. This review summarizes the last progresses in the bioreactor field, with main attention on the new configurations and the agitation systems, for conversion of dedicated energy crops (Arundo donax) and residual materials (corn stover, wheat straw, mesquite wood, agave bagasse, fruit and citrus peel wastes, sunflower seed hull, switchgrass, poplar sawdust, cogon grass, sugarcane bagasse, sunflower seed hull, and poplar wood) into sugars and ethanol. The main novelty of this review is its focus on reactor components and properties.

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Analysis of inducers of xylanase and cellulase activities production by Ganoderma applanatum LPB MR-56

Cited by 27 publications

References 13 publications

The Carbon Source Controls the Secretion and Yield of Polysaccharide-Hydrolyzing Enzymes of Basidiomycetes

The Carbon Source Controls the Secretion and Yield of Polysaccharide-Hydrolyzing Enzymes of Basidiomycetes

Screening and characterization of amylase and cellulase activities in psychrotolerant yeasts

Bioreactors for lignocellulose conversion into fermentable sugars for production of high added value products

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