Analysis of in vivo oocyte maturation, in vitro embryo development and gene expression in cumulus cells of dairy cows and heifers selected for one fertility quantitative trait loci (QTL) located on BTA3

Coyral-Castel, Stéphanie; Brisard, Daphné; Touzé, Jean-Luc; Dupont, Mickaël; Ramé, Christelle; Uzbekova, Svetlana; Dupont, Joëlle

doi:10.1016/j.theriogenology.2011.12.028

Cited by 10 publications

(7 citation statements)

References 48 publications

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“…We observed no differences in ovarian activity (number of follicles and follicular waves, length of estrus cycle) in 'fertil+' and 'fertil−' heifers and cows (Coyral-Castel et al 2011). However, we have demonstrated that the lower fertility of 'fertil−' females could be partially due to a lowest quality of the oocytes and consequently of pre-implantation embryo development (Coyral-Castel et al 2012). We have also characterized 'fertil+' and 'fertil−' cows for food intake and eating behavior, milk production, live weight and plasma metabolites during the first lactation.…”

Section: Introductioncontrasting

confidence: 55%

“…We have previously shown that Holstein cows selected for their homozygous favorable ('fertil+') haplotype at one QTL of female fertility located on the chromosome 3 (QTL-F-Fert-BTA3) had a 31% and 26% greater success rate at 35 and 90 days after the first artificial insemination, respectively, compared to the unfavorable ('fertil−') haplotype (Coyral-Castel et al 2011). Furthermore, we have observed slower oocyte maturation dynamics after in vivo maturation and lower blastocyst quality after in vitro embryo development in 'fertil−' compared to 'fertil+' heifers (Coyral-Castel et al 2012). In addition to fertility problems, we have shown that 'fertil−' cows had a lower body weight in the first eight weeks after calving than 'fertil+' cows and a more negative energy balance in the first week post partum, suggesting a greater fat mobilization in this haplotype (Coyral-Castel et al 2013).…”

Section: Discussionmentioning

confidence: 81%

“…So, KIRREL might not explain the better fertility in 'fertil+' as compared to 'fertil−' animals through its effects on the granulosa cells or oocyte. We have previously shown that progesterone secretion by cultured granulosa cells in basal state or in response to FSH or IGF1 was similar between 'fertil+' and 'fertil−' heifers submitted to ovarian stimulation (Coyral-Castel et al 2012). Thus, it will be interesting to know the role of KIRREL in vivo in ovarian functions in the bovine species.…”

Section: Discussionmentioning

confidence: 97%

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KIRREL is differentially expressed in adipose tissue from ‘fertil+’ and ‘fertil−’ cows: in vitro role in ovary?

et al. 2018

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We have previously shown that dairy cows carrying the 'fertil-' haplotype for one quantitative trait locus affecting female fertility located on the bovine chromosome three (QTL-F-Fert-BTA3) have a significantly lower conception rate and body weight after calving than cows carrying the 'fertil+' haplotype. Here, we compared by Tiling Array the expression of genes included in the QTL-F-Fert-BTA3 in 'fertil+' and 'fertil-' adipose tissue one week after calving when plasma non-esterified fatty acid concentrations were greater in 'fertil-' animals. We observed that thirty-one genes were overexpressed whereas twelve were under-expressed in 'fertil+' as compared to 'fertil-' cows ( < 0.05). By quantitative PCR and immunoblot we confirmed that adipose tissue mRNA and protein were significantly greater expressed in 'fertil+' than in 'fertil-'. mRNA is abundant in bovine kidney, adipose tissue, pituitary, and ovary and detectable in hypothalamus and mammary gland. Its expression (mRNA and protein) is greater in kidney of 'fertil+' than 'fertil-' cows ( < 0.05). KIRREL (mRNA and protein) is also present in the different ovarian cells with a greater expression in granulosa cells of 'fertil+' than 'fertil-' cows. In cultured granulosa cells, recombinant KIRREL halved steroid secretion in basal state ( < 0.05). It also decreased cell proliferation ( < 0.05) and oocyte maturation ( < 0.05). These results were associated to a rapid increase in MAPK1/3 and MAPK14 phosphorylation in granulosa cells and to a decrease in MAPK1/3 phosphorylation in oocyte. Thus, could be a potential metabolic messenger linking body composition and fertility.

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Section: Introductioncontrasting

confidence: 55%

Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 97%

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KIRREL is differentially expressed in adipose tissue from ‘fertil+’ and ‘fertil−’ cows: in vitro role in ovary?

et al. 2018

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“…We previously characterized 2 groups of primiparous Holstein cows differing on a female fertility QTL located on chromosome 3 (QTL-Fert-F-BTA3) and exhibiting either 69% (Fertil+ haplotype) or 39% (Fertil− haplotype) pregnancy rate at first service, as assessed by pregnancy diagnosis 35 d after AI (Coyral-Castel et al, 2011). These haplotypes were also shown to be different in their in vitro embryo development ability, the Fertil− embryo having developmental delay (Coyral-Castel et al, 2012a). The QTL-Fert-F-BTA3 is located between positions 9.8 and 13.5 cM (Guillaume et al, 2007;Ben Jemaa et al, 2008;Druet et al, 2008).…”

Section: Expression Of Adipokine and Lipid Metabolism Genes In Adiposmentioning

confidence: 99%

Expression of adipokine and lipid metabolism genes in adipose tissue of dairy cows differing in a female fertility quantitative trait locus

Elis

Coyral-Castel

Fréret

et al. 2013

Journal of Dairy Science

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We have previously characterized 2 haplotypes (Fertil+ and Fertil-) of Holstein dairy cows differing in 1 female fertility quantitative trait locus (QTL) located on chromosome 3 (QTL-Fert-F-BTA3) between positions 9.8 and 13.5 cM. This QTL is composed of 124 genes, some of them being involved in metabolism or reproduction. Primiparous Fertil+ and Fertil- cows exhibited 69 and 39% pregnancy rate at first service, respectively. A difference in plasma nonesterified fatty acid concentrations observed between both haplotypes might indicate a difference in adipose tissue mobilization. We compared adipose tissue gene expression in Fertil+ and Fertil- cows during their second lactation, at 2 physiological stages, implying either intense lipid mobilization (1 wk postpartum) or fat storage (5 mo of gestation). We investigated by reverse-transcription quantitative PCR the mRNA gene expression of 5 positional candidate genes located in the QTL-Fert-F-BTA3, as well as 18 other functional candidate genes encoding proteins involved in lipid metabolism and several adipokines. Among them, genes involved in either lipolysis or lipogenesis were chosen as controls because they were previously described in dairy cow adipose tissue. A hierarchical clustering was performed to group genes according to their expression pattern, allowing 2 clusters to be determined. Cluster 1 was composed of genes that were overexpressed during mobilization (ADIPOQ, ADIPOR2, LIPE, FABP4, PLIN1, RARRES, LEPR, and CPT1A) and cluster 2 of genes overexpressed during reconstitution of body reserves (ACACA, FASN, and SCD). Genes belonging to cluster 1 (LIPE, FABP4, PLIN1, and CPT1A) are known to be involved in lipolysis and fatty acid oxidation, and genes belonging to cluster 2 (ACACA, FASN, and SCD) are known to be involved in fatty acid synthesis. The expression of 5 genes from cluster 1 was correlated to plasma nonesterified fatty acid levels and thus to mobilization of body reserves in dairy cows (ADIPOQ, ADIPOR2, LIPE, PLIN1, and FABP4). During the mobilization stage, none of the positional candidate genes belonging to QTL-Fert-F-BTA3 (ADAR, MTX1, SHC1, SPTA1, and PAQR6) showed a difference in expression between the 2 haplotypes. Interestingly, ADIPOQ and ADIPOR2 were the only genes showing a significant mRNA overexpression in Fertil- cows at the mobilization stage. Further studies focusing on plasma adiponectin level and adipokine actions on the ovary are needed to investigate its potential role in dairy cow fertility.

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“…It is unlikely that this difference can explain the difference of fertility at first service between the two haplotypes. In a previous study, we have shown that the quality of oocytes could be different between the two haplotypes (Coyral-Castel et al, 2012). This difference could be due to differences in the gonadotropin secretions that we have not yet investigated.…”

Section: Discussionmentioning

confidence: 84%

Significant differences in fertility between dairy cows selected for one QTL located on bovine chromosome 3 are not attributable to energy balance, although eating behaviour is affected

Coyral-Castel

Faverdin

Ramé

et al. 2013

Animal

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animal Significant differences in fertility between dairy cows selected for one QTL located on bovine chromosome 3 are not attributable to energy balance, although eating behaviour is affected S. Coyral-Castel 1,2,3,4,5 , P. Faverdin 6,7 , C. Ramé 1,2,3,4 , S. Fré ret 1,2,3,4 , D. Guillaume Improvement of reproduction in dairy cows has become a major challenge in dairy production. We have recently shown that dairy cows carrying the 'fertil2' haplotype for one quantitative trait locus (QTL), affecting female fertility and located on the bovine chromosome 3, had a significantly lower conception rate after the first artificial insemination than cows carrying the 'fertil1' haplotype. The objective of this paper was to study other phenotypic modifications linked to this QTL. In the present study, 23 'fertil1' and 18 'fertil2' cows were characterized for live weight, milk production, food intake, eating behaviour and plasma metabolites. These parameters were measured during the first lactation, from calving to 40 weeks postpartum (wkpp). In the first 7 weeks of lactation, 'fertil1' primiparous cows had a significantly higher live BW and milk production than 'fertil2' cows. Dry matter intake tended to be slightly higher for 'fertil1' than for 'fertil2' primiparous cows in this period. However, energy balance was similar for the two haplotypes in the whole lactation, except in the first wkpp, and consequently, could not explain their different fertility. The major observation concerned the eating behaviour. 'Fertil1' primiparous cows had a significantly lower eating rate than 'fertil2' cows during the 40 weeks of lactation. In parallel, 'fertil1' cows spent significantly more time at the feeder for a similar number of visits than 'fertil2' cows. Furthermore, no differences in plasma concentrations of non-esterified fatty acids and insulin were observed between the two haplotypes. Plasma glucose was significantly lower in 'fertil1' than in 'fertil2' cows in the second wkpp. Taken together, our results show that 'fertil1' and 'fertil2' dairy cows, with different fertility, have also different eating behaviour without any variation in energy balance, except in the first week of lactation.Keywords: dairy cow, eating rate, quantitative trait locus, metabolite ImplicationsThe fall in dairy cows' fertility is related, in part, to the strong genetic selection for milk production. It has been shown that several bovine Quantitative Trait Loci (QTL) affected female fertility. However, other physiological roles of these QTLs are not clearly established. As the effect of a high milk production is associated with the negative energy balance at the beginning of lactation (high needs for energy and limited postpartum feed intake), we studied several zootechnical and metabolic parameters in dairy cows carrying contrasting haplotypes at one female fertility QTL located on the BTA3, and presenting a phenotypic difference in fertility. These experiments are of particularly strong interest with the development of genomic selection.

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Analysis of in vivo oocyte maturation, in vitro embryo development and gene expression in cumulus cells of dairy cows and heifers selected for one fertility quantitative trait loci (QTL) located on BTA3

Cited by 10 publications

References 48 publications

KIRREL is differentially expressed in adipose tissue from ‘fertil+’ and ‘fertil−’ cows: in vitro role in ovary?

KIRREL is differentially expressed in adipose tissue from ‘fertil+’ and ‘fertil−’ cows: in vitro role in ovary?

Expression of adipokine and lipid metabolism genes in adipose tissue of dairy cows differing in a female fertility quantitative trait locus

Significant differences in fertility between dairy cows selected for one QTL located on bovine chromosome 3 are not attributable to energy balance, although eating behaviour is affected

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