Analysis of impact of anaerobic condition on the aflatoxin production in Aspergillus parasiticus Speare

Abstract

Aflatoxins are the natural carcinogens that are the best characterized as fungal secondary metabolites. The producers that are responsible for aflatoxin biosynthesis are strongly associated in toxic contamination of essential agricultural products. Aspergillus parasiticus is an exclusive fungus that participates in causing hepatic problems in humans and cattle. These mycotoxins are greatly influenced by abiotic stresses. The fungal growth, proliferation and its toxigenicity are highly influenced by these stresses. Present study aimed to restrict the mycelial growth and to prevent aflatoxin preparation in A. parasiticus under the anoxic stress. The monosporic strains of A. parasiticus were grown in two different Erlenmeyer conical flasks containing Czapek Dox Broth and Czapek Dox Agar under both aerobic and anaerobic conditions. The anoxic condition was maintained using Anaero Bag System. Aflatoxin was isolated after 10 days, and quantitative estimation was done by using High Performance Liquid Chromatography (HPLC). The experimental outcome showed that there was a drastic decrease in both the morphological growth and the aflatoxin biosynthesis of A. parasiticus in anoxic state.

Molecular docking analysis of Omt-A protein model from Aspergillus flavus with synthetic compounds

“…In them, neoeriocitrin and blasticidin-S are the most efficient compounds, showing good docking results in many possible positions within the target protein. A more detailed structural view of the relationship can be made using hydrophobic interactions and hydrogen bonding interactions [ 15 ]. It is important to note that the blasticidin s that received the highest docking score appeared to block the targeted domain more effectively than any of the others.…”

RNAi: antifungal therapy against Invasive pulmonary aspergillosis (IPA) in humans

“…Due to the lack of an immediately available standard diagnostic test, the high mortality rate associated with IPA poses a significant problem. [33][34][35] Chen and his team initially worked on the inflammation due to IPA. They injected cyclophosphamide intraperitoneally into mice to create a neutropenic model and found that the mice's neutrophil count remained below 100/mm3 throughout the study.…”

Phyto-chemical screening of Root Extracts of Glycyrrhiza glabra by Spectroscopic Methods (UV-VIS Spectrophotometer, FTIR and HPLC)

“…The flow rate was kept at 1ml/min throughout the analysis and the acquisition was performed at 25ºC for 20 minutes. [23] Anti P. acne activity of glabridin The antimicrobial activity of glabridin against P. acne (ATCC 6919) was determined by well diffusion assay. The assay was performed on Muller Hinton Agar (MHA) in anaerobic condition.…”