Analysis of Immune Response of Chicken Primary Cells by Infection With Korean IBV Strain

Lee, Rangyeon; Jung, Jin‐Woo; Yeo, Ji-in; Kwon, Hyun-Jung; Park, Jeongho

doi:10.21203/rs.3.rs-201829/v1

2021

DOI: 10.21203/rs.3.rs-201829/v1

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Analysis of Immune Response of Chicken Primary Cells by Infection With Korean IBV Strain

Rangyeon Lee

Jin‐Woo Jung

Ji-in Yeo

et al.

Abstract: Infectious bronchitis virus (IBV), an avian coronavirus, is highly contagious, which develops acute pathogenesis in multiple organs. Frequent recombination of spike (S) glycoprotein leads to vaccine strategies insignificant. To understand IBV pathogenesis, we analyzed genetic distance of the Korean IBV isolate to different coronaviruses, including SARS-Cov2. For comprehensive information of the immune responses during IBV infection, we infected primary chicken embryonic kidney cells and performed transcriptome… Show more

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Cited by 2 publications

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Effects of bacteriophage supplement on the growth performance, microbial population, and PGC-1α and TLR4 gene expressions of broiler chickens

Sarrami

Sedghi

Mohammadi

et al. 2022

Sci Rep

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Bacteriophages (BP) are viruses that invade bacteria and propagate inside them, leading to the lysis of the bacterial cells. The aim of this study was to investigate the effect of adding BP to the broiler’s diet and its effect on the performance, morphology and bacterial population of the gut, some immune responses and expression of some intestinal genes. Accordingly, dietary treatments were as follows: basal diet (control), and control + 0.3 g/kg colistin or 0.5, 1 and 1.5 g BP/kg of diet. BP increased the body weight gain and reduced the feed conversion ratio (FCR), as compared to the colistin treatment, in the finisher and overall period (P < 0.05). European efficiency factor was significantly higher in 1.5 g BP-fed birds, as compared to the control and colistin treatments. meanwhile, bacteriophage and colistin-fed birds had higher Lactobacillus and lowered coliform bacteria counts, as compared to the control treatment (P < 0.05). Cecal concentrations of propionate in the 1.5 g BP-fed birds were higher than those in the control treatment (P < 0.05). BP-fed birds had a significantly increased villus height to crypt depth ratio, as compared to the control treatment. BP increased the serum concentrations of the total antibody, immunoglobulin (Ig) M, and IgG, as compared to the control treatment (P < 0.05). In the ileum, the expression of the Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) gene was decreased by dietary BP supplementation (P < 0.05). Furthermore, Toll-like receptor 4 (TLR4) gene expression was down-regulated in the BP-fed birds, whereas Interleukin 10 (IL-10) gene expression was up-regulated (P < 0.05). Overall, the use of BP may be a promising alternative to growth-promoting antibiotics in broilers by altering the gastrointestinal tract microbiota, enhancing immunological responses and improving the gut's morphology.

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Effects of bacteriophage supplement on the growth performance, microbial population, and PGC-1α and TLR4 gene expressions of broiler chickens

Sarrami

Sedghi

Mohammadi

et al. 2022

Sci Rep

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Effects of bacteriophage on Salmonella Enteritidis infection in broilers

Sarrami,

Sedghi,

Mohammadi

et al. 2023

Sci Rep

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Bacteriophages (BP) are viruses that can infect bacteria. The present study evaluated the effect of BP on Salmonella infected broilers. A number of 150 day-old broilers were used in a completely randomized design with five treatments that included: (1) basal diet from day 0 to 28; (2) basal diet + 0.3 g/kg of colistin from day 0 to 28; (3) basal diet from day 1 to 13, and basal diet + 0.4 g/kg of colistin from day 14 to 28; (4) basal diet + 1 g/kg of BP from day 0 to 28; (5) basal diet + 1.5 g/kg of BP from day 0 to 28. On day 13, 15 chickens from each treatment were challenged by Salmonella Enteritidis (SE), while fifteen from each treatment were not; instead, they were kept in the same cage with the challenged chickens (exposed chickens). At 7 and 14 days post-challenge, the number of SE and coliform bacteria in the cecum and liver of colistin and BP-fed birds was lower than the control treatment. In exposed and challenged chickens, the height and surface area of villus were greater in the BP and colistin-supplemented groups. Serum concentrations of aspartate aminotransferase and alanine transaminase were greater, while serum albumin and triglycerides concentrations were lower in the control treatment. The liver of the challenged chickens had more pathological lesions than exposed birds. BP significantly decreased PPARγ gene expression in exposed chickens. In the challenged and exposed chickens, TLR4 gene expression was lower in BP and colistin-treated birds as compared to the control. In conclusion, adding BP to the diet from the day of age prevents the spread of Salmonella.

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Analysis of Immune Response of Chicken Primary Cells by Infection With Korean IBV Strain

Cited by 2 publications

References 31 publications

Effects of bacteriophage supplement on the growth performance, microbial population, and PGC-1α and TLR4 gene expressions of broiler chickens

Effects of bacteriophage supplement on the growth performance, microbial population, and PGC-1α and TLR4 gene expressions of broiler chickens

Effects of bacteriophage on Salmonella Enteritidis infection in broilers

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