Analysis of immune cells draining from the abdominal cavity as a novel tool to study intestinal transplant immunobiology

Meier, Dominik; Cagnola, H.; Ramisch, Diego; Rumbo, Carolina; Chirdo, Fernando Gabriel; Docena, Guillermo Horacio; Gondolesi, Gabriel; Rumbo, Martı́n

doi:10.1111/j.1365-2249.2010.04192.x

Cited by 8 publications

(6 citation statements)

References 22 publications

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“…Instead, during histotropic phase of infection, we saw increased numbers of CD11b+Gr‐1+ cells in peritoneal fluid. Peritoneal cavity was shown to be an important trafficking site for intestinal immunity; therefore, parasite‐induced cells could migrate through the peritoneum to other lymphoid organs later during infection. Additionally, in our study, low count of MDSC‐like cells in local lymph nodes at 6th dpi coincided with an increased pool of the cells in the blood and the bone marrow.…”

Section: Discussionmentioning

confidence: 99%

Role of l‐arginine and CD11b+Gr‐1+ cells in immunosuppression induced by Heligmosomoides polygyrus bakeri

Brodaczewska

Donskow‐Łysoniewska

Krawczak

et al. 2020

Parasite Immunology

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Myeloid‐derived suppressor cells (MDSCs) are heterogeneous population of monocyte and granulocyte progenitors that are highly suppressive against T cells. In BALB/c mice infected with a nematode Heligmosomoides polygyrus bakeri, we studied the dynamics of MDSCs, identified as CD11b+Gr‐1+, induction in different tissues along with the development of parasite infection. We observed that MDSC‐like cells are induced both by larvae and adult stages of H polygyrus bakeri. Gr‐1+ cells of suppressive phenotype are recruited in the bone marrow, peripheral blood and peritoneal cavity during histotropic phase of infection and are present at that time in the intestine wall, where worms reside. Later, during intestinal phase, suppressive Gr‐1+ cells increased in mesenteric lymph nodes and the spleen. l‐arginine metabolism was important for the protective immunity, and parasite‐induced Gr‐1+ cells showed elevated arginase‐1 and iNOS expression. Inhibition of arginase‐1 and l‐arginine administration caused reduced level of infection that coincided with weaker suppressive phenotype of Gr‐1+ cells. We identified that l‐arginine pathway activation and induction of MDSC‐like cells characterize immunosuppressive state during H polygyrus bakeri infection in mice. Our findings confirm the role of MDSCs in parasitic infections and point l‐arginine pathway as a potential target for immunomodulation during nematode infections.

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Section: Discussionmentioning

confidence: 99%

Role of l‐arginine and CD11b+Gr‐1+ cells in immunosuppression induced by Heligmosomoides polygyrus bakeri

Brodaczewska

Donskow‐Łysoniewska

Krawczak

et al. 2020

Parasite Immunology

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“…Details of ITx surgical procedures, immunosuppressive therapy and patients' follow-up care were previously reported (20,21). Two different study groups were used.…”

Section: Patients and Study Groupsmentioning

confidence: 99%

Immunological Status of Isolated Lymphoid Follicles After Intestinal Transplantation

Meier

Docena

Ramisch

et al. 2014

American Journal of Transplantation

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Intestinal transplantation (ITx) faces the challenge of grafting a high immunogenic organ, which is certainly one of the major obstacles for intestinal allograft acceptance. The allograft has to guarantee the proper functioning of the mucosal immune machinery under immunosuppressive conditions. Recently, it has been elucidated that isolated lymphoid follicles (ILFs) are an indispensable part of mucosal immunity to maintain IgA synthesis and consequently to control commensal microflora. No data about these follicular structures in the setting of ITx are available so far. Therefore, we addressed the question whether constitution, integrity and function of allograft ILFs are disturbed by immunosuppressive regimen. We compared allograft ILFs from terminal ileum of transplant patients with ILFs from nontransplant patients via flow cytometry, quantitative real-time polymerase chain reaction and immunohistochemistry. We found that host leukocytes rapidly repopulate allograft ILFs and that maintenance immunosuppressive regimen, tacrolimus and corticosteroids, does not affect their cellular integrity and function. However, allograft ILFs revealed a higher maturation state than control samples and IgA positive plasma cells were increased in number in allograft mucosa. Our results open the path for a better understanding of allograft mucosal immunity.

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“…During the first two to three postoperative weeks, lymph containing immune cells from the mesentery is routinely collected by a drainage system in human transplant. We analyzed cellular content and found that the turnover from donor CD8 T cells to recipient T cells is completed during the second week posttransplant [93]. This analysis of gut draining cells might be interesting for studies of cellular subtypes with memory or regulatory functions and might provide a hint for future outcome of allograft survival.…”

Section: Secondary Lymphoid Structuresmentioning

confidence: 95%

Current Status of Allograft Tolerance in Intestinal Transplantation

Meier

Rumbo

Gondolesi

2013

International Reviews of Immunology

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Solid organ transplantation has become a clinical practice after the development of different immunosuppressive drugs that allowed controlling rejection. The price to be paid for that is the permanent risk of infections and malignancies and a significant drug-associated toxicity. The establishment of transplant tolerance has been the "holy grail" for transplantation medicine since its beginnings. Different experimental approaches and clinical trials resulted in the accumulation of knowledge on mechanisms and strategies that favor the establishment of tolerance without achieving the objective of autonomous allograft tolerance in the clinical field. Development of tolerance in intestinal transplantation constitutes a challenging situation due to several particular features that contribute to the generation of a strong allogeneic response. In the present review, we summarize the different immune mechanisms that may contribute to allograft tolerance. The different barriers that should be bypassed in intestinal transplantation to tolerate the graft are discussed. Finally, we revise the strategies that were applied with different degrees of success in the clinical field including the most promising recent approaches and the forthcoming candidates in the field that might be translated into clinical trials in the near future.

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Analysis of immune cells draining from the abdominal cavity as a novel tool to study intestinal transplant immunobiology

Cited by 8 publications

References 22 publications

Role of l‐arginine and CD11b+Gr‐1+ cells in immunosuppression induced by Heligmosomoides polygyrus bakeri

Role of l‐arginine and CD11b+Gr‐1+ cells in immunosuppression induced by Heligmosomoides polygyrus bakeri

Immunological Status of Isolated Lymphoid Follicles After Intestinal Transplantation

Current Status of Allograft Tolerance in Intestinal Transplantation

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