Analysis of IgE-Binding Factors (sCD23) within Newborn Sera

Abstract

factors are discussed as potential diagnostic parameters of atopic disorders. The amounts of sCD23 in sera from newborn children (n = 4,329) were determined by radioimmunoassay with monoclonal antibodies specific for CD23. The sCD23 levels ranged between 0 and 81.5 ng/ml of CD23-specific mAb. Furthermore, the sera of newborns with more than 5 ng/ml (n = 45) were analyzed by SDS/PAGE and subsequent autoradiography using ¹²⁵I-labeled IgE (PS). These experiments indicate that newborn sera with normal sCD23 amounts contain an IgE-binding activity with a molecular weight of 25 kD; this component was not observed within sera containing elevated amounts ( > 5 ng/ml). In addition, a 60-kD IgE-binding component was detected within most of all newborn sera (76.4%). The data show that the IgE-binding pattern of newborn children and the pattern of adult donors are different. Our data suggest that the measurement of quantitative sCD23 amounts combined with the analysis of the molecular weight pattern of the IgE-binding factors might be a helpful diagnostic parameter with regard to IgE-associated diseases.

Triggering through CD40 promotes interleukin‐4‐induced CD23 production and enhanced soluble CD23 release in atopic disease

“…Atopic diseases are characterized by the abnormal regulation of IgE production (reviewed in [l]). Expression of CD23, the low-affinity IgE receptor (FceRII), is increased in atopic disease [2,31 and is involved in the regulation of IgE production. CD23 is expressed on the surface of B cells, monocytes and activated T cells [4] and also exists as a family of soluble proteins derived from the surfacebound molecule, possibly due to an autoproteolytic mechanism [5].…”

Influence of Leukotrienes A₄ and B₄ on the Immunoglobulin Isotype Selection of Normal and Glucocorticoid-Treated Peripheral Blood Mononuclear Cells

“…,25I-labelled antibodies were prepared by the chloramine T method as described before [6]. The quantitation of CD23 on PBMC and purified cells was carried out as previously described [7]. The Ig(E, G, A, M) content of the culture supernatants was determined by a solid phase sandwich R1A as described earlier [5].…”