Analysis of Vibrio mimicus Clinical Strains by Arbitrarily Primed Polymerase Chain Reaction

Abstract

A total of 51 Vibrio mimicus clinical strains from different geographic locations were examined by arbitrarily primed polymerase chain reaction (AP-PCR). The primer VMH-3 divided them into 28 groups, although 18 groups consisted of a single strain at present. All groups had a common 1.0-kb amplification fragment. Most of the groups consisted of strains from same region, although two exceptional groups showed a few amplification fragments including strains from different regions. AP-PCR groups were not consistently associated with serogroups. AP-PCR is thought to be a valuable and easy method for the epidemiological study of V mimicus.

Molecular and Genomic Characterization of Vibrio mimicus Isolated from a Frozen Shrimp Processing Facility in Mexico

“…mimicus and is a useful marker for species identification [ 27 , 28 ]. Different studies have used some of these methods, but it has not been reported before, the simultaneous use of all these techniques for the purpose to study single species and establish which method is more useful to detected genetic variations [ 37 – 40 ]. In this study, ERIC-PCR and RAPD ( vmh 390R) were the most discriminatory techniques for establishing differences, by obtaining the lowest percentages of similarities between the analyzed strains, while GTG 5 -PCR was the less discriminatory method.…”

“…Bi et al . [ 37 ] used the arbitrarily primed polymerase chain reaction (AP-PCR) to study several clinical strains of V . mimicus , and when comparing their results with those of this study, a similar number of band patterns were found, with the exception that their amplified products were of larger size.…”

Búsqueda fenotípica y genotípica de factores de virulencia no descritos en Vibrio mimicus

“…En el Cuadro 3 se observan los resultados globales, en donde de se observa que se han encontrado características que no habían sido descritas para V. mimicus y que el origen de las cepas es independiente de la distribución de los marcadores. Esto coincide con la propuesta de Chowdhury y colaboradores (1986), quienes afirman que V. mimicus es un grupo heterogéneo, en donde cada cepa tiene capacidades patogénicas diferentes y que éstas están relacionadas con la presencia o ausencia de factores de virulencia (Bi et al, 2000;Faruque et al, 2003;Faruque y Mekalanos, 2003;Li et al, 2000;Shi et al, 1998;Shinoda et al, 2004), en donde el ejemplo más claro lo tenemos al comparar las cepas silvestres con la cepa de referencia que no presenta el fenotipo ni el genotipo del pili.…”