Analysis of trp repressor‐DNA interactions using gel electrophoresis

Abstract

Quantitative analysis of the DNA-binding equilibria of E. coli trp repressor by gel electrophoresis led to reevaluation of our understanding of this complex system. In this review, the data leading to controversy about the trp system are discussed, and our current understanding is presented.

Cooperative and Anticooperative Effects in Binding of the First and Second Plasmid OsymOperators to a LacI Tetramer: Evidence for Contributions of Non-operator DNA Binding by Wrapping and Looping

“…l repressor; Koblan & Ackers, 1991;Senear & Batey, 1991) and/or weaker DNA binding specificities (e.g. trp repressor; Lewis & Carey, 1993;Liu & Matthews, 1993), making consideration of association or dissociation equilibria of these proteins more important.…”