Analysis of<i>Leptospira</i>isolates from mainland Portugal and the Azores islands

Collares-Pereira, Margarida; Korver, H.; Thi, B.V Cao; Santos‐Reis, Margarida; Bellenger, E.; Baranton, Guy; Terpstra, W. J.

doi:10.1111/j.1574-6968.2000.tb09059.x

Cited by 23 publications

(5 citation statements)

References 18 publications

(31 reference statements)

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“…Their reactions to the mAbs specific for the Pomona serogroup were very similar to each other but different from any of the known serogroup Pomona strain profiles. Their pattern was compatible with serovar Tsaratsovo, which was first isolated from harvest mice (Micromys minutus) in 1962, in the Plovdiv District of Bulgaria by Ivanov and later found in rodents in some parts of Europe [66]. Nevertheless, only mAbs F48 C6 and F61 C7, included in our panel, were tested on this serovar.…”

Section: Discussionsupporting

confidence: 64%

Serological Survey and Molecular Typing Reveal New Leptospira Serogroup Pomona Strains among Pigs of Northern Italy

et al. 2020

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Swine act as both maintenance and incidental hosts of pathogenic Leptospira spp. Here, a serological test was performed on 131,660 pig sera collected between 2002 and 2017 from 4715 farms in Northern Italy. A positivity rate of 13.05% was determined. Australis was the most frequently identified serogroup (77.29%), followed by Pomona (18.47%), Tarassovi (1.51%) and Icterohaemorrhagie (1.40%). Culture isolation and real-time Polymerase chain reaction (PCR) were carried out on 347 kidneys and 470 clinical samples, respectively. Overall, 133 strains were cultured successfully and 43 randomly chosen isolates were identified as serogroup Pomona. Multi-locus sequence typing (MLST) revealed that 41 isolates and 8 DNA extracted from biological samples belonged to sequence type 140. Using a multiple-locus, variable-number tandem repeat analysis, 43 samples produced identical profiles but, after 2014, three new Leptospira interrogans serogroup Pomona genotypes were observed. Interestingly, two isolates showed new MLST profiles and an unclassified identification by monoclonal antibodies. The 16S rRNA gene sequencing clustered them into L. kirschneri species and a core genome MLST analysis revealed an allelic identity of 96% compared with Mozdok strains. Genotyping allowed us to discriminate leptospires and to identify new emerging strains. The accurate identification of infective strains is required for formulating preventive methods and intervention strategies.

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Section: Discussionsupporting

confidence: 64%

Serological Survey and Molecular Typing Reveal New Leptospira Serogroup Pomona Strains among Pigs of Northern Italy

et al. 2020

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“…Further REA typing of the field strains used in the study showed the same Eco RI and Hha I restriction patterns for all tested isolates, and they were compatible with the DNA profiles of those four very closely related serovars. These results correlated with those of some previous studies, revealing difficulties in genetic discrimination between those strains ( 8 , 17 – 19 ). Moreover, using monoclonal antibody typing, Kobayashi et al ( 22 , 23 ) also showed a very close antigenic similarity of those strains, which led the authors to put those four serovars in one subgroup.…”

Section: Discussionsupporting

confidence: 91%

The serological and genetic diversity of the Leptospira interrogans Icterohaemorrhagiae serogroup circulating in the UK

Arent,

Gilmore,

Pardyak

et al. 2023

Journal of Veterinary Research

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Introduction Strains of Leptospira interrogans belonging to two very closely related serovars, Icterohaemorrhagiae and Copenhageni, have been associated with disease in mammalian species and are the most frequently reported agents of human leptospirosis. They are considered the most pathogenic serovars and represent more than half of the leptospires encountered in severe human infections. Material and Methods Nineteen such isolates from the United Kingdom – human, domestic and wildlife species – were typed using three monoclonal antibodies (F12 C3, F70 C14 and F70 C24) in an attempt to elucidate their epidemiology. They were further examined by restriction endonuclease analysis (REA), multiple-locus variable-number tandem repeat analysis (MLVA) and lic12008 gene sequence analysis. Results Monoclonal antibody F12 C3, which is highly specific for Icterohaemorrhagiae and Copenhageni, confirmed that all the strains belonged to these two serovars. Sixteen strains were identified as Copenhageni and three as Icterohaemorrhagiae serovar. Only one restriction pattern type was identified, thus confirming that REA is not able to discriminate between the Icterohaemorrhagiae and Copenhageni serovars. Variable-number tandem-repeat analysis found three loci with differences in the repeat number, indicating genetic diversity between British isolates. Sequences of the lic12008 gene showed that all isolates identified as the Icterohaemorrhagiae serotype have a single base insertion, in contrast to the same sequences of the Copenhageni serotype. Conclusion Copenhageni is the predominant serovar in the Icterohaemorrhagiae serogroup isolated in British Isles. There is a genetic diversity of MLVA patterns of the isolates but no genetic tool used in the study was able to determine serovars.

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“…The MST method permits the strains of the Copenhageni and Icterohaemorrhagiae serovars to be differentiated into five distinct profiles, in contrast to the unique profile by VNTR analysis (18). These two serovars are particularly difficult to discriminate when using other typing methods, such as PFGE (34), VNTR (35), and MLST (36). This MST capacity is particularly interesting for the epidemiologic surveillance of the L. interrogans species carried in rodents, such as Rattus norvegicus, since they are considered the main environmental spreader of zoonotic Leptospira species.…”

Section: Discussionmentioning

confidence: 99%

High-Resolution Typing of Leptospira interrogans Strains by Multispacer Sequence Typing

et al. 2014

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Leptospirosis is a worldwide zoonosis which is responsible for the typical form of Weil's disease. The epidemiological surveillance of the Leptospira species agent is important for host prevalence control. Although the genotyping methods have progressed, the identification of some serovars remains ambiguous. We investigated the multispacer sequence typing (MST) method for genotyping strains belonging to the species Leptospira interrogans, which is the main agent of leptospirosis worldwide. A total of 33 DNA samples isolated from the reference strains of L. interrogans serogroups Icterohaemorrhagiae, Australis, Canicola, and Grippotyphosa, which are the most prevalent serogroups in France, were analyzed by both the variable-number tandem-repeat (VNTR) and MST methods. An MST database has been constructed from the DNA of these reference strains to define the MST profiles. The MST profiles corroborated with the VNTR results. Moreover, the MST analysis allowed the identification at the serovar level or potentially to the isolate level for strains belonging to L. interrogans serovar Icterohaemorrhagiae, which then results in a higher resolution than VNTR (Hunter-Gaston index of 0.94 versus 0.68). Regarding L. interrogans serogroups Australis, Canicola, and Grippotyphosa, the MST and VNTR methods similarly identified the genotype. The MST method enabled the acquisition of simple and robust results that were based on the nucleotide sequences. The MST identified clinical isolates in correlation with the reference serovar profiles, thus permitting an epidemiological surveillance of circulating L. interrogans strains, especially for the Icterohaemorrhagiae serogroup, which includes the most prevalent strains of public health interest.

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Analysis ofLeptospiraisolates from mainland Portugal and the Azores islands

Cited by 23 publications

References 18 publications

Serological Survey and Molecular Typing Reveal New Leptospira Serogroup Pomona Strains among Pigs of Northern Italy

Serological Survey and Molecular Typing Reveal New Leptospira Serogroup Pomona Strains among Pigs of Northern Italy

The serological and genetic diversity of the Leptospira interrogans Icterohaemorrhagiae serogroup circulating in the UK

High-Resolution Typing of Leptospira interrogans Strains by Multispacer Sequence Typing

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