Analysis of
            <i>FUSOBACTERIUM NUCLEATUM</i>
            and
            <i>Streptococcus Gallolyticus</i>
            in Saliva of Colorectal Cancer Patients

Güven, Deniz Can; Dizdar, Ömer; Alp, Alpaslan; Kıttana, Fatma Nur Akdoğan; Karakoç, Derya; Hamaloğlu, Erhan; Laçin, Şahin; Karakaş, Yusuf; Kılıçkap, Saadettin; Hayran, Mutlu; Yalçın, Şuayib

doi:10.2217/bmm-2019-0020

Cited by 28 publications

(29 citation statements)

References 68 publications

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“…S1). Similarly, higher amount of F. nucleatum in saliva was found in patients with colorectal cancer (Guven et al, 2019). Interestingly, we have also recently reported that F. nucleatum was highly FIG.…”

Section: Discussionmentioning

confidence: 60%

Fusobacterium nucleatumCaused DNA Damage and Promoted Cell Proliferation by theKu70/p53Pathway in Oral Cancer Cells

Geng

Zhang

et al. 2020

DNA and Cell Biology

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Bacterial infection influences genomic stability and integrity by causing DNA damage, which increases the possibility of tumor initiation and development. We aimed to investigate whether Fusobacterium nucleatum, one of the periodontal pathogens, promoted oral squamous cell carcinoma (OSCC) by causing DNA doublestrand break (DSB). Tca8113 tongue squamous cell carcinoma cells were infected with F. nucleatum. The expression of gH2AX was detected by western blots and immunofluorescence. The proliferation and cell cycle alterations were tested by CCK8 and flow cytometry, respectively. The expression levels of Ku70, p53, and p27 were evaluated by quantitative real-time polymerase chain reaction and western blots. A plasmid was used for the overexpression of Ku70 to verify the possible relationship between Ku70 and p53. We confirmed the presence of DSBs in the response to F. nucleatum by detecting the expression of gH2AX. The cell proliferation ability was increased with an accelerated cell cycle while the expression of p27 was decreased. Meanwhile, the expression of Ku70 and wild p53 was downregulated. When Ku70 was overexpressed, the expression of wild p53 in response to F. nucleatum infection was upregulated and cell proliferation was accordingly inhibited. We concluded that F. nucleatum infection promoted the proliferation ability of Tca8113 by causing DNA damage via the Ku70/p53 pathway.

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Section: Discussionmentioning

confidence: 60%

Fusobacterium nucleatumCaused DNA Damage and Promoted Cell Proliferation by theKu70/p53Pathway in Oral Cancer Cells

Geng

Zhang

et al. 2020

DNA and Cell Biology

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“…As for searching candidate salivary biomarkers, Fn, Pg, Dd and Pm were selected as qPCR targets in our cohort based on previous literatures. One recent study reported that salivary Fn level in CRC group was higher compared with the control group, while the amount of salivary Pg was similar between the two groups 42 . However, the sample size included was limited which need further validation.…”

Section: Discussionmentioning

confidence: 86%

Salivary Desulfovibrio Desulfuricans Level and Oral Hygiene Index for the Prediction of Colorectal Cancer in Chinese Patients

Wang

Zhang

Wang

et al. 2020

Preprint

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Background: Mounting evidence have shown that fecal microbiome can act as biomarkers for diagnosing colorectal cancer (CRC). Recent studies demonstrate that oral microbiome is concordant with gut microbiome. The role of oral microbiome in colorectal cancer has not been fully illustrated. Methods: We collected preoperational saliva with a final cohort of 237 patients who underwent surgical resections or colorectal endoscopy in XX Hospital from January 2018 to January 2020. Clinical demographics, comorbidities and oral conditions were obtained from medical records or questionnaires. Salivary microbial biomarkers were detected by quantitative polymerase chain reaction (PCR) after DNA extraction. Multivariate logistics regression analysis was employed to analyze the risk factors for colorectal cancer. A four-variable prediction model was constructed based on the logistics analysis.Results: Among the 237 patients enrolled, there were 95 endoscopy confirmed healthy control and 142 pathologically confirmed colorectal adenocarcinoma patients. Logistics regression analysis demonstrated that the risk factors associated with CRC included age at diagnosis (OR=1.111, 95%CI=1.072-1.151), male sex (OR=2.111, 95%CI=1.068-4.175), oral hygiene index (OR=1.769, 95%CI=1.116-2.804) and relative salivary Desulfovibrio desulfuricans (Dd) abundance (OR=1.156, 95%CI=1.05-1.272), based on which a four-variable model was developed. The four-variable model had good discriminative (Brier score=0.144, Concordance index=0.866) and calibration (0.834) abilities after bias correction. Conclusions: Elevated salivary Dd level is an independent risk factor for CRC. We have developed a four-variable model that could help identify at-risk patients for CRC.

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“…This finding also merits further exploration in a larger study. Cohort studies in pancreatic and orodigestive cancers have found increased risk for incidence and mortality with high levels of P. gingivalis antibodies in serum, [1] and similarly high levels of F. nucleatum in saliva appear to be associated with colon cancer risk [40].…”

Section: Discussionmentioning

confidence: 99%

Feasibility of investigating the association between bacterial pathogens and oral leukoplakia in low and middle income countries: A population-based pilot study in India

et al. 2021

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Background Certain oral bacterial pathogens may play a role in oral carcinogenesis. We assessed the feasibility of conducting a population-based study in India to examine the distributions and levels of Porphyromonas gingivalis, Fusobacterium nucleatum and Prevotella intermedia in relation to oral leukoplakia (a potentially malignant disorder) and other participant characteristics. Methods This exploratory case-control study was nested within a large urban Indian cohort and the data included 22 men and women with oral leukoplakia (cases) and 69 leukoplakia-free controls. Each participant provided a salivary rinse sample, and a subset of 34 participants (9 cases; 25 controls) also provided a gingival swab sample from keratinized gingival surface for quantitative polymerase chain reaction (qPCR). Results Neither the distribution nor the levels of pathogens were associated with oral leukoplakia; however, individual pathogen levels were more strongly correlated with each other in cases compared to controls. Among controls, the median level of total pathogens was the highest (7.55×104 copies/ng DNA) among persons of low socioeconomic status. Salivary rinse provided better DNA concentration than gingival swab for qPCR analysis (mean concentration: 1.8 ng/μl vs. 0.2 ng/μl). Conclusions This study confirms the feasibility of population studies evaluating oral microbiome in low-resource settings and identifies promising leads for future research.

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Analysis of FUSOBACTERIUM NUCLEATUM and Streptococcus Gallolyticus in Saliva of Colorectal Cancer Patients

Cited by 28 publications

References 68 publications

Fusobacterium nucleatumCaused DNA Damage and Promoted Cell Proliferation by theKu70/p53Pathway in Oral Cancer Cells

Fusobacterium nucleatumCaused DNA Damage and Promoted Cell Proliferation by theKu70/p53Pathway in Oral Cancer Cells

Salivary Desulfovibrio Desulfuricans Level and Oral Hygiene Index for the Prediction of Colorectal Cancer in Chinese Patients

Feasibility of investigating the association between bacterial pathogens and oral leukoplakia in low and middle income countries: A population-based pilot study in India

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