Analysis of
            <i>Bacillus subtilis tagAB</i>
            and
            <i>tagDEF</i>
            Expression during Phosphate Starvation Identifies a Repressor Role for PhoP∼P

Liu, W; Eder, S.; Hulett, F. Marion

doi:10.1128/jb.180.3.753-758.1998

Cited by 72 publications

(48 citation statements)

References 26 publications

(34 reference statements)

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“…To date no definitive function has been attributed to teichoic acid and this fact, in conjunction with its indispensable phenotype; frame an interesting problem in Gram-positive physiology. In B. subtilis 168 at least 10 biosynthetic enzymes, the majority of which are genetically organized in divergent transcriptional units and subject to complex regulation (Mauel et al, 1994;Liu et al, 1998;Howell et al, 2003), catalyse the formation of a polymer that comprises up to half of the cell wall material. Despite all of the investigation into the chemical nature and biosynthesis of teichoic acid, its indispensability cannot be attributed to anything beyond its localization to the cell wall.…”

Section: Wall Teichoic Acid Biosynthesis and Exportmentioning

confidence: 99%

Cell wall assembly in Bacillus subtilis: how spirals and spaces challenge paradigms

Bhavsar

Brown

2006

Molecular Microbiology

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SummaryAlthough the bacterial cell wall has been the subject of decades of investigation, recent studies continue to generate novel and controversial models of its synthesis and assembly. Here we compare and contrast the transcompartmental biosyntheses of peptidoglycan and teichoic acid in Bacillus subtilis . In addition, the current paradigms of B. subtilis wall assembly and structure are distinguished from emerging models of murein insertion and organization. We discuss evidence for the directed, cytoskeleton-dependent insertion of nascent peptidoglycan and the existence of a periplasmic compartment. Furthermore, we summarize the challenges these findings represent to the existing paradigm of murein insertion. Finally, motivated by these new developments, we discuss outstanding issues that remain to be addressed and suggest research directions that may contribute to a better understanding of cell wall assembly in B. subtilis .

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Section: Wall Teichoic Acid Biosynthesis and Exportmentioning

confidence: 99%

Cell wall assembly in Bacillus subtilis: how spirals and spaces challenge paradigms

Bhavsar

Brown

2006

Molecular Microbiology

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“…Inactivation of MecA results in overproduction of ComK [10,11,15], leading to inhibition of the c D -dependent degR expression [16]. Recently Liu and Zuber [17] demonstrated that ComK positively regulates the transcription of the £gM-containing operon that encodes an anti-c D factor FlgM which binds to c D [18^21]. Thus the expression of the c D regulon is negatively regulated by ComK.…”

Section: Introductionmentioning

confidence: 99%

Positive regulation of Bacillus subtilis sigD by C‐terminal truncated LacR at translational level

et al. 1999

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DegR is a positive regulator for degradative enzyme synthesis in Bacillus subtilis. The degR gene is transcribed by RNA polymerase containing c c D , and the level of its expression is low in a mecA-deficient mutant. In a search for suppressors of the mecA effect through mini-Tn10 transposon mutagenesis, a lacR mutation designated lacR288 was discovered. The B. subtilis lacR gene encodes the repressor for lacA which specifies L L-galactosidase, and therefore, inactivation of the lacR gene results in overproduction of the enzyme. In the lacR288 mutant, however, the expression of lacA was at a negligible level, indicating that the repressor activity was not destroyed by the mutation. The putative gene product of the lacR288-containing gene is a 288-amino acid protein lacking the C-terminal 42 amino acids of intact LacR and carries no extra amino acids derived from the transposon sequence. The suppression by lacR288 of the decreased degR expression in the mecA background was found to be caused by an increase in the c c D level as shown by Western blot analysis. Furthermore, the increase was due to post-transcriptional regulation of sigD, the gene encoding c c D , as revealed by using both transcriptional and translational sigD-lacZ fusions. The lacR288 mutation had no effect on the stability of the c c D protein. Based on these results we conclude that the lacR288 mutation stimulates sigD expression at the translational level.z 1999 Federation of European Biochemical Societies.

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“…PhoR, a histidine kinase, senses a signal and is autophosphorylated under phosphate starvation conditions, or in the presence of ATP in vitro, and it then transfers the phosphoryl group to PhoP, a response regulator. Phosphorylated PhoP (PhoPϳP) acts as a transcriptional activator or repressor of Pho regulon genes (operons), including phoA, phoB, pstS, phoD, tuaA, tagA, and tagD (1,2,8,9,11,13,20). DNase I footprinting data showed that both PhoP and PhoPϳP bound to a PhoP core binding region located between positions Ϫ22 and Ϫ60 in all known Pho regulon promoters (11)(12)(13)(14), while PhoPϳP also bound to sites in the coding regions of some Pho regulon genes (14).…”

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confidence: 99%

Role of PhoP∼P in Transcriptional Regulation of Genes Involved in Cell Wall Anionic Polymer Biosynthesis in Bacillus subtilis

Ying

Hulett

1998

J Bacteriol

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tagA, tagD, and tuaA operons are responsible for the synthesis of cell wall anionic polymer, teichoic acid, and teichuronic acid, respectively, in Bacillus subtilis. Under phosphate starvation conditions, teichuronic acid is synthesized while teichoic acid synthesis is inhibited. Expression of these genes is controlled by PhoP-PhoR, a two-component system. It has been proposed that PhoP∼P plays a key role in the activation oftuaA and the repression of tagA andtagD. In this study, we demonstrated the role of PhoP∼P in the switch process from teichoic acid synthesis to teichuronic acid synthesis, by using an in vitro transcription system. The results indicate that PhoP∼P is sufficient to repress the transcription of the tagA and tagD promoters and also to activate the transcription of the tuaA promoter.

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Analysis of Bacillus subtilis tagAB and tagDEF Expression during Phosphate Starvation Identifies a Repressor Role for PhoP∼P

Cited by 72 publications

References 26 publications

Cell wall assembly in Bacillus subtilis: how spirals and spaces challenge paradigms

Cell wall assembly in Bacillus subtilis: how spirals and spaces challenge paradigms

Positive regulation of Bacillus subtilis sigD by C‐terminal truncated LacR at translational level

Role of PhoP∼P in Transcriptional Regulation of Genes Involved in Cell Wall Anionic Polymer Biosynthesis in Bacillus subtilis

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