Analysis of human leukaemias and lymphomas using extensive immunophenotypes from an antibody microarray

Belov, Larissa; Mulligan, Stephen P.; Barber, Nicole; Woolfson, Adrian; Scott, Mike; Stoner, Kerryn; Chrisp, Jeremy S.; Sewell, William A.; Bradstock, Kenneth F.; Bendall, Linda J.; Pascovici, Dana; Thomas, Mervyn; Erber, Wendy N.; Huang, Pauline; Sartor, Mary; Young, G. A. R.; Wiley, James S.; Juneja, Surender; Wierda, William G.; Green, Anthony; Keating, Michael J.; Christopherson, Richard I.

doi:10.1111/j.1365-2141.2006.06266.x

Cited by 63 publications

(47 citation statements)

References 35 publications

(42 reference statements)

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“…By contrast, DotScan TM enables simultaneous and highly parallel screening of many (currently 82-147) CD antigens, and this extensive surface immuno-profiling appears sufficient to classify most human leukaemias. The CD antigens identified by the microarray correspond well with those identified by flow cytometry [2,3] although different parameters are measured. In the case of flow cytometry, the fluorescence intensity per cell is assessed on a gated population of cells and compared with the level of fluorescence for control antibodies.…”

Section: Classification Of Leukaemiasmentioning

confidence: 69%

“…The surface profiles of the monocyte-and macrophage-like cells induced by 1,25D 3 and TPA, respectively, are not identical to those obtained from normal monocytes and macrophages [17]. However, Fig.…”

Section: -O-tetradecanoyl Phorbol-13-acetatementioning

confidence: 70%

“…Cell surface profiles for AML showed higher levels of surface CD117 (c-kit) from BMA compared with PB. For ALL, CD34 levels were higher for precursor B-ALL from BMA compared with PB [3]. These differences indicate that BMA samples contained a greater proportion of immature leukaemias; CD117 and CD34 expression is lost during maturation of myeloid (CD117 and CD34) and lymphoid (CD34) cells.…”

Section: Surface Immuno-profiling Of Leukaemia Samples From Peripheramentioning

confidence: 94%

“…We have used DotScan TM to compile a database of mononuclear leukocyte immunophenotypes from peripheral blood or bone marrow aspirates derived from patients with a range of different leukaemias [3]. AML comprises a number of diseases and other groups have attempted to define AML sub-types using surface immuno-profiling.…”

Section: Classification Of Leukaemiasmentioning

confidence: 99%

“…The human cell line HL-60, is derived from a case of the M2 subtype of AML and is used as a model for drug-induced differentiation of promyelocytic leukaemia [16]. Drugs such as ATRA, 12-O-tetradecanoyl phorbol-13-acetate (TPA) or 1-a,25-dihydroxyvitamin D 3 (1,25D 3 ) induce HL-60 cells to differentiate into mature myeloid cells with loss of proliferative capacity [17]. ATRA induces major changes in membrane proteins, some of which are adhesion molecules that interact with neighbouring cells.…”

Section: All-trans Retinoic Acidmentioning

confidence: 99%

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Profiling CD antigens on leukaemias with an antibody microarray

Barber¹,

Gez²,

Belov³

et al. 2009

FEBS Letters

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Edited by Miguel De la RosaKeywords: Leukaemia Lymphoma Cluster of differentiation antigen Microarray a b s t r a c t Cluster of differentiation (CD) antigens are defined when a surface molecule found on some members of a standard panel of human cells reacts with at least one novel antibody, and there is good accompanying molecular data. Monoclonal antibodies to surface CD antigens on leukocytes have been used for flow cytometry, and more recently to construct microarrays that capture live cells. These DotScan TM microarrays enable the rapid and highly parallel characterization of repertoires of CD antigens whose expression patterns may be correlated with discrete leukaemia subtypes, or used to define biomarker 'signatures' for non-hematological diseases. DotScan TM with fluorescence multiplexing enables profiling of CD antigens for minor subsets of cells, such as colorectal cancer cells and tumour-infiltrating lymphocytes from a surgical sample.

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Section: Classification Of Leukaemiasmentioning

confidence: 69%

Section: -O-tetradecanoyl Phorbol-13-acetatementioning

confidence: 70%

Section: Surface Immuno-profiling Of Leukaemia Samples From Peripheramentioning

confidence: 94%

Section: Classification Of Leukaemiasmentioning

confidence: 99%