Analysis of HSD11B2 as a prognostic marker in Melanoma via TCGA data mining

Abstract

Background: Hydroxysteroid 11-Beta Dehydrogenase 2 (HSD11B2) expression has been reported to be present in melanoma. We investigated the association of HSD11B2 with melanoma using publicly available data from The Cancer Genome Atlas (TCGA). Methods: The relationship between clinical pathologic features and HSD11B2 were analysed via Wilcoxon signed-rank test and logistic regression. Clinicopathologic characteristics associated with overall survival in melanoma patients were calculated using Cox regression and the Kaplan-Meier method. Gene Set Enrichment Analysis (GSEA) and gene co-association of HSD11B2 were performed using TCGA data set. Results: Reduced HSD11B2 expression was significantly lower in melanoma patients compared to normal patients (p value = 3.004e-122) and also associated with lower survivability. low HSD11B2 expression in melanoma was also significantly associated with cancer stages T (p value = 0.002) and N (p value < 0.001) and age (p value = 0.003). Genes TFCP2L1, PRR15L, ATP6V1B1, C9orf152, AC009948.1, AL391244.1, WDCP, HNRNPCP2 and GTF2E1 were all shown to be co-associated with changes in HSD1B2 expression. Multiple signalling pathway including cytosolic DNA sensing pathway, JAK STAT signalling pathway, NOD like receptor signalling pathway, T cell receptor signalling pathway and Toll like receptor signalling pathway were differentially enriched in low HSD11B2 expression phenotype. Conclusion: Our study revealed that HSD11B2 expression is closely associated with melanoma development and age, as well as multiple cancer related genes and pathways, thus highlighting HSD11B2 as a potential therapeutic marker of melanoma.

Construction and validation of a glycolysis-related lncRNA signature for prognosis prediction in Stomach Adenocarcinoma

“…In this study, a total of seven glycolysis-related lncRNAs (AL353804.1, AC010719.1, TNFRSF10A-AS1, AC005586.1, AL355574.1, AC009948.1, and AL161785.1) with prognostic significance in STAD were identified using Lasso and Cox regression analyses. Among them, the two glycolysis-related LncRNAs (TNFRSF10A-AS1 and AC009948.1) have been reported in previous studies (FU et al, 2020;Wei et al, 2020). In a study by Wei et al (Xia et al, 2021), TNFRSF10A-AS1 was identified as an autophagy-related lncRNA associated with the poor prognosis of colorectal cancer, and along with other seven autophagy-related lncRNAs, it constructed a prognostic signature for colorectal cancer, which was used to predict the prognosis in colorectal cancer patients.…”

“…These findings also suggested that the TNFRSF10A-AS1 might participate in a variety of biological processes, affecting the prognosis of various tumors, and is worthy of further exploration in subsequent studies with good research potential. Fu et al (FU et al, 2020) found that AC009948.1 was co-expressed with HSD11B2, which is related to the progression and prognosis of melanoma as demonstrated by mining the data of melanoma in TCGA. The GSEA analysis suggested that HSD11B2 was related to multiple cancer-related genes and pathways, including cytosolic DNA-sensing pathway, JAK_STAT signaling pathway, T-cell receptor signaling pathway, and Toll-like receptor signaling pathway.…”