Analysis of how the production and activity of PGD2 affects glioma cell lines.

Ferreira, Matthew Thomas

doi:10.11606/d.42.2015.tde-10042015-120110

Cited by 1 publication

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“…Em trabalhos anteriores do nosso laboratório, foi visto que a adição de PGD 2 em condições fisiológicas nas linhagens A172, U-87MG e U-251MG, aumentou a proliferação das linhagens celulares em comparação aos seus controles (Ferreira, 2014).…”

Section: Lc/esi-ms/ms Nas Linhagens De Gbmunclassified

Análise do perfil dos prostanoides e do seu papel no controle da migração celular em glioblastoma.

Gomes¹

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Gomes RG. Analysis of the profile of prostanoids and their role in the control of cell migration in glioblastoma. [Ph.D Thesis (Cell and Tissue Biology)]. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo; 2016. Glioblastoma (GBM; astrocytoma grade IV WHO) is the most common and aggressive brain tumour in adults. Despite intensive treatments involving surgical resection, radiotherapy and chemotherapy, most patients diagnosed with GBM have a median survival of only 1 year. The difficulties involved in the success of treatments are largely due to the migratory and invasive capacity of the GBM tumour cells to invade surrounding brain tissue. The prostanoids (PGE 2 , PGD 2 , PGF 2α , PGI 2 e TXA 2) are part of a family of biologically active lipids derived from arachidonic acid (AA) that are involved in various physiological and pathological functions. The increased production of these prostanoids, especially PGE 2 , is involved in many developmental processes and cancer progression, such as proliferation, apoptosis and angiogenesis. PGE 2 , through different signaling pathways is known to influence the process of cell migration of various tumours. However, in GBM the role of these prostanoids is poorly understood. The aim of this study was to analyse the in vitro profile of different prostanoids in the cell lines of GBM (T98G, A-172, U-138MG, U-251MG and U-87MG), as well as analysing the role of prostanoids and their receptors in the migration of the U-251MG and U-87MG cell lines. The results showed a varied production of series 2 prostanoids among the different cell lines, and a lack of detection of series 1 and 3 prostanoids. The expression of COX-1, COX-2, mPGES1, mPGES2, cPGES, EP2, EP4, PGT and 15-HPGD was observed by real-time PCR and by immunofluorescence. In migration assays treatment with exogenous prostanoids increased the migration of U-251MG and U-87MG cell lines. Treatment with the EP2 receptor antagonist (AH6809) and EP4 receptor antagonist (L161.982) caused a decrease in the migration of both cell lines in comparison with their respective controls. Together these results demonstrate the important role of prostanoids, especially PGE 2 , in the migration process of GBM cells. This effect is mediated, at least in part, by a change in cellular response, following the activation of the EP2 and EP4 receptors. .

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Section: Lc/esi-ms/ms Nas Linhagens De Gbmunclassified

Análise do perfil dos prostanoides e do seu papel no controle da migração celular em glioblastoma.

Gomes¹

View full text Add to dashboard Cite

show abstract

Analysis of how the production and activity of PGD2 affects glioma cell lines.

Cited by 1 publication

References 68 publications

Análise do perfil dos prostanoides e do seu papel no controle da migração celular em glioblastoma.

Análise do perfil dos prostanoides e do seu papel no controle da migração celular em glioblastoma.

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