Analysis of hop acids by thin-layer chromatography and the Molecular Ionization Desorption Analysis Source (MIDAS) for mass spectrometry

Winter, Gregory T.; Wilhide, Joshua A.; LaCourse, William R.

doi:10.1016/j.ijms.2017.08.013

Cited by 7 publications

(1 citation statement)

References 11 publications

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“…One approach to reduce ion suppression and matrix effects is to combine ADI sources with planar chromatography techniques such as thin-layer chromatography (TLC) or high-performance thin-layer chromatography (HPTLC). For example, the benefit of coupling TLC to DESI-MS [ 15 – 17 ], DART-MS [ 18 – 20 ], low-temperature plasma (LTP) [ 21 ], and molecular ionization-desorption analysis source (MIDAS) [ 22 , 23 ] has been demonstrated in several studies. Other plasma-based ADI sources such as the flowing atmospheric-pressure afterglow (FAPA) source were used in TLC-ADI-MS studies as well [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Quantitative detection of caffeine in beverages using flowing atmospheric-pressure afterglow (FAPA) ionization high-resolution mass spectrometry imaging and performance evaluation of different thin-layer chromatography plates as sample substrates

2022

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Ambient desorption/ionization mass spectrometry (ADI-MS) is widely used as a rapid screening tool of samples in their native state without sample preparation. While analysis times are much less than 1 min per sample, one challenge of ADI-MS is the possibility to perform quantitative analysis of analytes in complex matrices. Typically, the goal is to probe a variety of different analytes in a complex matrix from a solid, liquid, or otherwise uncharacterized surface in the open air in front of the MS inlet. In this study, it is demonstrated that a carefully selected surface for analyte spot sampling and co-deposited isotopically labeled standards both significantly improve the capabilities of flowing atmospheric-pressure afterglow (FAPA) high-resolution (HR) MS for direct quantitative analysis. Specifically, a systematic study of different surfaces (glass, steel mesh, high-performance thin-layer chromatography (HPTLC) stationary phases including silica, reversed-phase (RP)-modified silica, and cyano (CN)-modified silica) and their suitability for spot sampling with FAPA-MS was performed. A set of different caffeine-containing standards and beverages (Red Bull, Coca-Cola, coffee, and black tea) was deposited on the surfaces and direct FAPA-HR-MS analysis of caffeine was performed using internal calibration with co-deposited 13C3-caffeine. For TLC surfaces, it was demonstrated that quantitative results could be achieved with the matrix and concomitants present and that a preceding chromatographic separation was not mandatory for this application. In addition, the use of a CN-HPTLC surface resulted in a significantly more intense caffeine signal in the beverage samples compared to the other surfaces studied, with the highest increase compared to the silica (200-fold higher) and the lowest increase compared to the steel mesh (30-fold higher). The utilization of TLC-based surfaces as sample carriers is considered an attractive tool in the ADI-MS toolbox for fast and efficient mass spectrometric investigations of complex samples without time-consuming sample preparation. Graphical Abstract

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