Analysis of Hepatic Lipid Metabolism and Immune Function During the Development of Collagen-Induced Arthritis

Abstract

The liver is essential for metabolic and immune functions and has been linked to systemic inflammatory diseases. However, the role of the liver is still elusive during the development of rheumatoid arthritis (RA), although there have been indeed some reports. We used label-free quantitative proteomics and experimental verification in this study to reveal the hepatic lipid metabolism and immune function during collagen-induced arthritis (CIA) development. The proteomics results revealed that the role of the liver differs in different phases of CIA rats. In terms of specific performance, hepatic lipid metabolism, which is primarily concerned with cholesterol, triacylglycerol, and phospholipid, was significantly influenced in the CIA induction phase, whereas the immune function, which includes binding of granulocytes, adhesion of immune cells, etc., was affected considerably at the peak phase of CIA rats compared to normal rats. Finally, the hepatic dynamic changes in CIA rats were further confirmed using targeted metabolomics and ELISA. We found that most fatty acids of the liver in the CIA induction phase were significantly decreased, and proteins related to complement activation and migration or adhesion of immune cells including C3, MMP-8, CTSZ, and S100A9 were significantly increased in the liver of CIA rats in the peak phase. Our findings indicated that the lipid metabolism and immune function of the liver were influenced in CIA rats. Thus, the conditions of the liver during RA development should be considered in therapeutic and nutritional interventions.

Inhibition of protein arginine deiminase 4 prevents inflammation-mediated heart failure in arthritis

“…There is evidence of elevated immune cell infiltration in the livers, kidneys, and lungs of arthritic mice (35,36). Inhibition of PAD4 could therefore not only be beneficial to the primary disease but several collateral comorbidities of RA as well.…”

“…Histological analysis suggested that the functional improvement was likely because of the prevention of myocardial hypertrophy and myocardial fibrosis (Fig 5D and F). Mice treated with JBI-589 had lower heart weight-to-tibia length ratio (9 [±2] mg/mm versus 11 [±2] mg/mm; P = 0.004) and reduced LVM measured by echocardiography (33 [27][28][29][30][31][32][33][34][35][36][37][38][39] mg versus 74…”

A Metabolic Driven Bio‐Responsive Hydrogel Loading Psoralen for Therapy of Rheumatoid Arthritis

“…Establishment and Evaluation of CIA: The RA rat models were established using previously described methods and protocols using bovine type II collagen and complete Freund's adjuvant. [27] 24 male Sprague-Dawley rats were randomly divided into four groups as follows (n = 6): Control, CIA, free psoralen, and psoralen gel groups. The free psoralen and psoralen gel groups were treated via intra-articular injection, in which the psoralen gel group was injected with 50 µL psoralen gel, and the free psoralen group was injected the same dose of psoralen without the hydrogel sustained-release system.…”

Correlation Of Lipid Profile and Das28 Score of Early Rheumatoid Arthritis Patients on Conventional Synthetic Dmards

“…Moreover, RA is associated with increased incidence and mortality rates of CVD. In recent times, the interplay between lipid metabolism and RA has garnered increasing attention (4). Conventional synthetic disease-modifying antirheumatic drugs (DMARDs) play a crucial role in the treatment of inflammatory arthritides, including RA.…”