Analysis of heat, cold or salinity stress-inducible genes in the Pacific abalone, Haliotis discus hannai, by suppression subtractive hybridization

Abstract

In order to investigate environmental stress inducible genes in abalone, we analyzed differentially expressed transcripts from a Pacific abalone, Haliotis discus hannai, after exposure to heat-, cold-or hyposalinity-shock by suppression subtractive hybridization (SSH) method. 1,074 unique sequences from SSH libraries were composed to 115 clusters and 986 singletons, the overall redundancy of the library was 16.3%. From the BLAST search, of the 1,316 ESTs, 998 ESTs (75.8%) were identified as known genes, but 318 clones (24.2%) did not match to any previously described genes. From the comparison results of ESTs pattern of three SSH cDNA libraries, the most abundant EST was different in each SSH library: small heat shock protein p26 (sHSP26) in heat-shock, trypsinogen 2 in cold-shock, and actin in hyposalinity SSH cDNA library. Based on sequence similarities, several response-to-stress genes such as heat shock proteins (HSPs) were identified commonly from the abalone SSH libraries. HSP70 gene was induced by environmental stress regardless of temperature-shock or salinity-stress, while the increase of sHSP26 mRNA expression was not detected in cold-shock but in heat-shock condition. These results suggest that the suppression subtractive hybridization method is an efficient way to isolate differentially expressed gene from the invertebrate environmental stress-response transcriptome.

Cloning and expression analysis of HSP70 gene from mangrove plant Kandelia obovata under cold stress

“…KoHsp70 will be to play a stress-associated role in the later period under cold stress. There were similar responses under cold shock that observed in other organisms (Li et al 2013, Nam et al 2013, Guo et al 2014, Jensen et al 2014, Mohanarao et al 2014.…”

Expression of Heat Shock Proteins in Thermally Challenged Pacific Abalone Haliotis discus hannai

“…Similarly, HSP90 was more sensitive to acute thermal stress then to chronic thermal stress, which was observed in the gill of Haliotis discus [16,17], while HSP70 was observed to be sensitive to acute and chronic thermal stress in Haliotis rufescens [33,34]. Among the sHSPs, HSP20 and HSP26 were significantly expressed in muscle and mantle tissue of H. discus under thermal stress [22,35].…”