Analysis of Hazardous Biological Materials by MALDI Mass Spectrometry

Wahl, Karen L.; Jarman, Kristin H.; Valentine, Nancy B; Kingsley, Mark T; Petersen, Catherine E; Wunschel, Sharon C; Saenz, Adam J

doi:10.2172/15002705

Cited by 1 publication

(2 citation statements)

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“…Previous efforts to assess reproducibility associated with MALDI profiling of bacteria have been reported . Many have relied upon visual inspection of spectra as well as peak location, intensity, and presence/absence of particular peaks . Recent approaches have involved calculating intra‐run coefficients of variations (CVs), combined ANOVA‐PCA analyses, and, most recently, using the BioNumerics software package (Applied Maths, Sint‐Martens‐Latem, Belgium) to facilitate rigorous quantitative data analysis .…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Automating data acquisition affects mass spectrum quality and reproducibility during bacterial profiling using an intact cell sample preparation method with matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry

Schumaker

Borror

Sandrin

2012

Rapid Comm Mass Spectrometry

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The use of matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry (TOF MS) has emerged as a promising tool to rapidly profile bacteria at the genus and species level and, more recently, at the sub-species (strain) level. Recently, it has been proposed that the approach can be enhanced with regard to reproducibility and throughput by automating spectrum acquisition; however, effects of automating spectrum acquisition on spectrum quality and reproducibility have not been investigated. Using an intact cell-based sample preparation method, we directly compared the quality and reproducibility of spectra acquired in a fully automated fashion to those acquired manually by two operators with different levels of experience. While automation tended to increase base peak resolution, other measures of spectrum quality, including signal-to-noise (S:N) ratio, data richness, and reproducibility were reduced. Negative effects of automation on the performance of this approach to bacterial profiling may be particularly important during profiling of closely related strains of bacteria that yield very similar spectra.

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Section: Resultsmentioning

confidence: 99%

“…Standard methods have been proposed recently that, if widely adopted, might enhance the reproducibility of MALDI‐TOF MS‐enabled microbial fingerprinting . As has been noted previously, reproducibility has been quantified rigorously in only a few studies …”

Section: Strain Designations Gram Reactions Cell Morphologies Biocmentioning

confidence: 99%