With the advantages of uniform stress transfer and weight reduction, adhesive joints are widely used in engineering. The propagation of non-linear Lamb waves in an adhesive joint with micro-cracks distributing in a random way is systematically investigated by using the numerical simulation method in this paper. A finite element model of the tri-layer adhesive structure with micro-cracks distributing randomly is established, and the Lamb wave mode pair with a matching condition of the phase velocity is chosen to examine the interaction of the micro-cracks with Lamb waves. The results show that the micro-cracks within the adhesive layer will lead to the generation of second harmonics. We also find that the Acoustic Non-linearity Parameters (ANP) increase with the propagation distance in the micro-crack damage zone and the density of the micro-cracks. However, ANPs are less concerned with the friction coefficients of the surface of micro-cracks. This numerical research reveals that non-linear Lamb waves can be employed to effectively characterize the micro-cracks related damages within an adhesive joint.