Analysis of glycopeptide biomarkers by on-line TiO2 solid-phase extraction capillary electrophoresis-mass spectrometry

Mancera-Arteu, Montserrat; Lleshi, Nejsi; Sanz‐Nebot, Victoria; Giménez, Estela; Benavente, Fernando

doi:10.1016/j.talanta.2019.120563

Cited by 21 publications

(21 citation statements)

References 33 publications

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“…An online TiO 2 microcartridge SPE was applied for analysis of O ‐glycopeptides released by the tryptic digest of recombinant human erythropoietin [80]. It is used in quality control of this biopharmaceutical and in doping analysis.…”

Section: Sample Preparationmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Kašička

2021

Electrophoresis

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The review provides a comprehensive overview of developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, microscale isolation, and physicochemical characterization of peptides from 2019 up to approximately the middle of 2021. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis, such as sample preparation, sorption suppression, EOF control, and detection, are presented. New developments in the individual CE and CEC methods are demonstrated and several types of their applications are shown. They include qualitative and quantitative analysis, determination in complex biomatrices, monitoring of chemical and enzymatic reactions and physicochemical changes, amino acid, sequence, and chiral analyses, and peptide mapping of proteins. In addition, micropreparative separations and determination of significant physicochemical parameters of peptides by CE and CEC methods are described.

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Section: Sample Preparationmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Kašička

2021

Electrophoresis

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“…Compared to the analysis of released N -glycans, the bottom-up approach allows to characterize the protein N -glycan pool with respect to the site occupancy. The same considerations are also relevant to its biomarker potential, as glycopeptides are gaining an increased attention in diagnostics of cancer and other diseases and are investigated as target substrates in cancer immunotherapy and immunodiagnostics. − …”

Section: Introductionmentioning

confidence: 99%

Site-Specific N-Linked Glycosylation Analysis of Human Carcinoembryonic Antigen by Sheathless Capillary Electrophoresis–Tandem Mass Spectrometry

et al. 2021

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With 28 potential N -glycosylation sites, human carcinoembryonic antigen (CEA) bears an extreme amount of N -linked glycosylation, and approximately 60% of its molecular mass can be attributed to its carbohydrates. CEA is often overexpressed and released by many solid tumors, including colorectal carcinomas. CEA displays an impressive heterogeneity and variability in sugar content; however, site-specific distribution of carbohydrate structures has not been reported so far. The present study investigated CEA samples purified from human colon carcinoma and human liver metastases and enabled the characterization of 21 out of 28 potential N -glycosylation sites with respect to their occupancy. The coverage was achieved by a multienzymatic digestion approach with specific enzymes, such as trypsin, endoproteinase Glu-C, and the nonspecific enzyme, Pronase, followed by analysis using sheathless CE-MS/MS. In total, 893 different N -glycopeptides and 128 unique N -glycan compositions were identified. Overall, a great heterogeneity was found both within (micro) and in between (macro) individual N -glycosylation sites. Moreover, notable differences were found on certain N -glycosylation sites between primary adenocarcinoma and metastatic tumor in regard to branching, bisection, sialylation, and fucosylation. Those features, if further investigated in a targeted manner, may pave the way toward improved diagnostics and monitoring of colorectal cancer progression and recurrence. Raw mass spectrometric data and Skyline processed data files that support the findings of this study are available in the MassIVE repository with the identifier MSV000086774 [DOI: ].

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“…This is in keeping with a report by Aykut and colleagues 16 who treated Ti wires with a methanolic solution of Teic, the resultant material of which was capable of killing S. aureus and preventing Ti infection in a rabbit model. Titania (TiO 2 ) is the natural surface finish of the metal and a compound widely used as a chromatography sorbent for the enrichment and purification of glycopeptides 24 – 26 .…”

Section: Discussionmentioning

confidence: 99%

“…In addition, no studies were forthcoming to ascertain the robustness of the interaction between the surface and the antibiotic by performing mock bacterial cultures or leaching experiments. These considerations are particularly important given that glycopeptides bind avidly to TiO 2 25 26 , 29 .…”

Section: Discussionmentioning

confidence: 99%

Immobilised teicoplanin does not demonstrate antimicrobial activity against Staphylococcus aureus

Britton

Lee

Azizova

et al. 2022

Sci Rep

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Antibacterial bone biomaterial coatings appeal to orthopaedics, dentistry and veterinary medicine. Achieving the successful, stable conjugation of suitable compounds to biomaterial surfaces is a major challenge. A pragmatic starting point is to make use of existing, approved antibiotics which are known to remain functional in a stationary, immobilised state. This includes the macrocyclic glycopeptide, teicoplanin, following the discovery, in the 1990’s, that it could be used as a chiral selector in chromatographic enantiomeric separations. Importantly teicoplanin works at the level of the bacterial cell wall making it a potential candidate for biomaterial functionalisations. We initially sought to functionalise titanium (Ti) with polydopamine and use this platform to capture teicoplanin, however we were unable to avoid the natural affinity of the antibiotic to the oxide surface of the metal. Whilst the interaction between teicoplanin and Ti was robust, we found that phosphate resulted in antibiotic loss. Before contemplating the covalent attachment of teicoplanin to Ti we examined whether a commercial teicoplanin stationary phase could kill staphylococci. Whilst this commercially available material could bind N-Acetyl-L-Lys-D-Ala-D-Ala it was unable to kill bacteria. We therefore strongly discourage attempts at covalently immobilising teicoplanin and/or other glycopeptide antibiotics in the pursuit of novel antibacterial bone biomaterials.

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Analysis of glycopeptide biomarkers by on-line TiO2 solid-phase extraction capillary electrophoresis-mass spectrometry

Cited by 21 publications

References 33 publications

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Site-Specific N-Linked Glycosylation Analysis of Human Carcinoembryonic Antigen by Sheathless Capillary Electrophoresis–Tandem Mass Spectrometry

Immobilised teicoplanin does not demonstrate antimicrobial activity against Staphylococcus aureus

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