Analysis of global Aeromonas veronii genomes provides novel information on source of infection and virulence in human gastrointestinal diseases

Abstract

Background Aeromonas veronii is a Gram-negative rod-shaped motile bacterium that inhabits mainly freshwater environments. A. veronii is a pathogen of aquatic animals, causing diseases in fish. A. veronii is also an emerging human enteric pathogen, causing mainly gastroenteritis with various severities and also often being detected in patients with inflammatory bowel disease. Currently, limited information is available on the genomic information of A. veronii strains that cause human gastrointestinal diseases. Here we sequenced, assembled and analysed 25 genomes (one complete genome and 24 draft genomes) of A. veronii strains isolated from patients with gastrointestinal diseases using combine sequencing technologies from Illumina and Oxford Nanopore. We also conducted comparative analysis of genomes of 168 global A. veronii strains isolated from different sources. Results We found that most of the A. veronii strains isolated from patients with gastrointestinal diseases were closely related to each other, and the remaining were closely related to strains from other sources. Nearly 300 putative virulence factors were identified. Aerolysin, microbial collagenase and multiple hemolysins were present in all strains isolated from patients with gastrointestinal diseases. Type III Secretory System (T3SS) in A. veronii was in AVI-1 genomic island identified in this study, most likely acquired via horizontal transfer from other Aeromonas species. T3SS was significantly less present in A. veronii strains isolated from patients with gastrointestinal diseases as compared to strains isolated from fish and domestic animals. Conclusions This study provides novel information on source of infection and virulence of A. veronii in human gastrointestinal diseases.

Phylogenomics of novel clones ofAeromonas veroniirecovered from a freshwater lake reveals unique biosynthetic gene clusters

“…Interestingly, one isolate from this study (NB-188) was nested with an isolate recovered from an aquatic ecosystem in France. A previous study assessing the core genome-based phylogenetic analysis of A. veronii genomes deposited in NCBI from 18 countries revealed high genetic diversity (5). The admixture of A. veronii strains from different sources was observed, suggesting a lack of source- and timepoint-based clustering in the A. veronii population.…”

A Novel Transposon Tn7709 Harbors Multidrug Resistance Genes in a Pathogenic Aeromonas media Strain QST31

“…Types II (T2SS), III (T3SS), IV (T4SS), and VI (T6SS) are the common four secretion systems in Aeromoas , and T2SS is responsible for the secretion of toxins, such as aerolysin, protease, and amylases. T3SS is often found in pathogenic Aeromonas , such as A. veronii [ 18 , 19 ], A. hydrophila [ 17 ], and A. salmonicida [ 20 ], which are closely related to the virulence of pathogens. T6SS can inject effector proteins into the host cells, which plays an important role in bacterial competition [ 3 ].…”

Genomic Characterization of Aeromonas veronii Provides Insights into Taxonomic Assignment and Reveals Widespread Virulence and Resistance Genes throughout the World

“…Among the most important species of the genus is Aeromonas veronii, a mobile mesophilic bacterium that causes furunculosis and motile aeromonas Antibiotics 2023, 12, 1039 2 of 18 septicemia (MAS) in fish, affecting commercial fish farms and causing economic losses to aquaculture [3,4]. In addition, the role of this species as a pathogen capable of causing intestinal diseases in humans has been increasingly highlighted [5,6].…”