Analysis of genomic characteristics and their influence on metabolism in Aspergillus luchuensis albino mutants using genome sequencing

Yamamoto, Nozomi; Watarai, Naoki; Koyano, Hitoshi; Sawada, Kazunori; Toyoda, Atsushi; Kurokawa, Ken; Yamada, Takuji

doi:10.1016/j.fgb.2021.103601

Cited by 7 publications

(3 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PP-binding domains are involved in transporting the secondary metabolite substrate and chain intermediates to the catalytic centers during the biosynthesis of polyketides ( Pihet et al., 2009 ). Interestingly, a portion of the pksP PP-binding domain in Aspergillus luchuensis was also deleted in industrial and artificially mutated albino strains compared to strains with wild-type spore color, which suggests a critical functional role for this domain in PksP function ( Yamamoto et al., 2021 ). Additionally, previous studies have established the essentiality of the thioesterase domain in PksP for naphthopyrone synthesis ( Vagstad et al., 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative Genomics Reveals a Single Nucleotide Deletion in pksP That Results in White-Spore Phenotype in Natural Variants of Aspergillus fumigatus

Gibbons

D’Avino

Zhao

et al. 2022

Front. Fungal Biol.

View full text Add to dashboard Cite

Aspergillus fumigatus is a potentially deadly opportunistic human pathogen. A. fumigatus has evolved a variety of mechanisms to evade detection by the immune system. For example, the conidium surface is covered in a layer of 1,8-dihydroxynaphthalene (DHN) melanin which masks the antigen macrophages use for recognition. DHN melanin also protects conidia from ultraviolet radiation and gives A. fumigatus conidia their characteristic green-grayish color. Here, we conducted genomic analysis of two closely related white-spore natural variants of A. fumigatus in comparison to two closely related green-spore isolates to identify a genetic basis of the white-spore phenotype. Illumina whole-genome resequencing data of the four isolates was used to identify variants that were shared in the white-spore isolates and different from both the green-spore isolates and the Af293 reference genome (which is also a green-spore isolate). We identified 4,279 single nucleotide variants and 1,785 insertion/deletions fitting this pattern. Among these, we identified 64 variants predicted to be high impact, loss-of-function mutations. One of these variants is a single nucleotide deletion that results in a frameshift in pksP (Afu2g17600), the core biosynthetic gene in the DHN melanin encoding gene cluster. The frameshift mutation in the white-spore isolates leads to a truncated protein in which a phosphopantetheine attachment site (PP-binding domain) is interrupted and an additional PP-binding domain and a thioesterase domain are omitted. Growth rate analysis of white-spore and green-spore isolates at 37°C and 48°C revealed that white-spore isolates are thermosensitive. Growth rate of A. fumigatus Af293 and a pksP null mutant in the Af293 background suggests pksP is not directly involved in the thermosensitivity phenotype. Further, our study identified a mutation in a gene (Afu4g04740) associated with thermal sensitivity in yeasts which could also be responsible for the thermosensitivity of the white-spore mutants. Overall, we used comparative genomics to identify the mutation and protein alterations responsible for the white-spore phenotype of environmental isolates of A. fumigatus.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative Genomics Reveals a Single Nucleotide Deletion in pksP That Results in White-Spore Phenotype in Natural Variants of Aspergillus fumigatus

Gibbons

D’Avino

Zhao

et al. 2022

Front. Fungal Biol.

View full text Add to dashboard Cite

show abstract

“…kawachii IFO 4308 (NBRC 4308) [4] . In addition, genome sequences of four other white koji fungi have recently been reported [5] . However, as these sequences were incomplete draft genome assemblies, we conducted a chromosome-level genome analysis of strain IFO 4308.…”

Section: Data Descriptionmentioning

confidence: 99%

Chromosome-level genome sequence data and analysis of the white koji fungus, Aspergillus luchuensis mut. kawachii IFO 4308

Mori¹,

Kadooka²,

Oda³

et al. 2022

Data in Brief

View full text Add to dashboard Cite

“…Initial studies on A. luchuensis as white koji fungus are used in the production of shochu, a traditional Japanese liquor [11][12][13] for its ability to prevent microbial spoilage and increase the aroma of white wine [12,14]. Recent studies on A. luchuensis have been conducted on the genes encoding the enzymes involved in the metabolism of tea phenolic compounds when fermenting tea leaves [10], genomic characteristics [15], denti cation and characterisation of thermostable endo-polygalacturonase II B [16], as well as the secondary metabolites [17]. However, the few attempts on probiotic properties, safety, antibacterial and antioxidant properties of A. luchuensis as a probiotic have been focused on.…”

Section: Introductionmentioning

confidence: 99%

Probiotic Potential, Antibacterial, and Antioxidant Capacity of Aspergillus luchuensis YZ-1 Isolated From Liubao Tea

Zhang¹,

Jiao²,

Li³

et al. 2023

Probiotics & Antimicro. Prot.

View full text Add to dashboard Cite

Aspergillus fungi are widely used in the traditional fermentation of food products, so their safety risks and functions are worthy of investigation. In this study, one Aspergillus luchuensis YZ-1 isolated from Liubao tea was identi ed based on phylogenetic analyses of sequences of three genes coding for internal transcribed spacer 1 (ITS1), β-tubulin (benA) and calmodulin (CaM). The results of hemolytic activity, Dnase activity, cytotoxicity assay and antibiotic resistance assay indicated that the strain is potentially safe. The excellent gastrointestinal uids tolerance, acid tolerance, bile tolerance, auto-aggregation, coaggregation, cell surface hydrophobicity and adhesion to human colon adenocarcinoma (HT29) cell line were observed on analysis of the probiotic properties. Furthermore, the results of the antibacterial activity of A. luchuensis YZ-1 indicated that the strain had strong antagonistic effects against Gram-negative and Gram-positive bacteria as well as fungi. Simultaneously, the water extracts and 80% ethanolic extracts of A. luchuensis YZ-1 cells also showed strong ABTS, DPPH and OH − scavenging ability. Taken together, our results suggest that A. luchuensis YZ-1 has desirable functional probiotic properties and can be proposed as a biocontrol agent in the food industry.

show abstract

Analysis of genomic characteristics and their influence on metabolism in Aspergillus luchuensis albino mutants using genome sequencing

Cited by 7 publications

References 58 publications

Comparative Genomics Reveals a Single Nucleotide Deletion in pksP That Results in White-Spore Phenotype in Natural Variants of Aspergillus fumigatus

Comparative Genomics Reveals a Single Nucleotide Deletion in pksP That Results in White-Spore Phenotype in Natural Variants of Aspergillus fumigatus

Chromosome-level genome sequence data and analysis of the white koji fungus, Aspergillus luchuensis mut. kawachii IFO 4308

Probiotic Potential, Antibacterial, and Antioxidant Capacity of Aspergillus luchuensis YZ-1 Isolated From Liubao Tea

Contact Info

Product

Resources

About