Analysis of genetic variation within and among Ulva pertusa (Ulvaceae, Chlorophyta) populations using ISSR markers

Genetic analyses of floating Ulva prolifera in the Yellow Sea suggest a unique ecotype

“…The genomic DNA from each tubular thallus (approximately 5 cm) was separated using a modified CTAB method (Zhao et al, 2010). The quality and concentration of the obtained DNA were tested by comparison with the lDNA/EcoR I þ Hind III markers (Fermentas, Shanghai, China) on a 0.8% agarose gel.…”

Genetic variation of Ulva (Enteromorpha) prolifera (Ulvales, Chlorophyta)—the causative species of the green tides in the Yellow Sea, China

“…The inter-simple sequence repeat (ISSR) method is considered suitable for research on genetic diversity within a species because of its high level of variety. Therefore, it has been used in studies on the genetic structure of many species in red algae , brown algae (Wang et al 2006;Zhao et al 2008), and green algae (Zhao et al 2010). The principal goal of the present study was to provide information on the genetic variation of both attached and floating U. prolifera samples from China according to ISSR markers that have been proven efficacious for ploidy state-unknown algal individuals .…”

The yellow sea green tides were dominated by one species, Ulva (Enteromorpha) prolifera, from 2007 to 2011

“…Genome DNA was extracted by a modified CTAB method [31]. PCR amplification of the ITS region (including ITS1, 5.8S, and ITS2) was performed using the primers and thermal cycling profile of Leskinen et al [32].…”