Analysis of genetic variability of<i>Fusarium oxysporum</i>f. sp.<i>cepae</i>the causal agent of basal rot on onion using RAPD markers

Malathi, S.; Mohan, S.

doi:10.1080/03235408.2012.680036

Cited by 4 publications

(3 citation statements)

References 10 publications

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“…Cramer et al (2003) characterized genetic diversity and pathogenicity of 166 isolates of F. oxysporum from bean and sugar beet plants by 12 RAPD primers generating 105 polymorphic bands. Cluster analysis from RAPD analysis clearly separated isolates into two clusters A and B confirming genetic diversity among the isolates of F. oxysporum from onion (Malathi and Mohan 2012). Genetic diversity among isolates from maize pathogen was found to be very high but specific relation was not observed between vegetative compatibility groups and their geographical origin (Mohammadi and Mofrad 2011).…”

Section: Discussionmentioning

confidence: 84%

Morphological, pathological and mycotoxicological variations among Fusarium verticillioides isolated from cereals

2018

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Among the 194 isolates screened from 127 cereal samples, 176 were fumonisin producers and others were non-producers. Representative nine strains along with one reference standard strain MTCC156 were selected to study their morphological, pathological and mycotoxicological variations by conventional and molecular approaches. strains FVM86, FVM146, FV200 and FVS3 showed significant pathogenicity and also in pigmentation production but varied in fumonisin production. strain FVP19 recorded variations in all the assays. strain FVM42 showed drastic phenotypic variation and it also produced fumonisin. Genetic variation among the strains was independent of geographic area of origin but depended on their ability to produce fumonisin. The strains were independent in their cultural characteristics, pigmentation production, pathogenicity assays, fumonisin production and in their genetic variability without having any correlation.

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Section: Discussionmentioning

confidence: 84%

Morphological, pathological and mycotoxicological variations among Fusarium verticillioides isolated from cereals

2018

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“…Since the disease was reported, F. oxysporum f. sp. cepae has been known to be the causal agent of basal rot of onion [ 21 , 37 , 38 , 39 ] in India. Shamyuktha et al [ 39 ] recorded 27.00 to 74.33% F. oxysporum f. sp.…”

Section: Discussionmentioning

confidence: 99%

Active Prevalence of Fusarium falciforme and F. acutatum Causing Basal Rot of Onion in Maharashtra, India

Dutta,

Jayalakshmi,

Radhakrishna

et al. 2024

JoF

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Over the past decade, there have been accumulating reports from researchers, farmers, and field extension personnel on the increasing incidence and spread of onion basal rot in India. Onion basal rot disease is mainly caused by Fusarium spp. This study aimed to validate the information on the active prevalence of F. falciforme and F. acutatum causing Fusarium basal rot (FBR) in Maharashtra. A survey was conducted, and the infected plants/bulbs were collected from fields of 38 locations comprising five districts of Maharashtra, namely, Nashik, Aurangabad, Solapur, Ahmednagar, and Pune, in 2023. This disease was prevalent in high-moisture and high-oil-temperature conditions and the symptoms were observed in most of the fields, with the FBR incidence ranging from 17 to 41%. The available data of basal rot incidence from 1998 to 2022 were analyzed, based on which the prevalence of FBR was 11–50%. Tissue from the infected samples of onion bulbs was used for the isolation. The identification was performed based on colony morphology and microscopic features and confirmed through molecular markers using ITS and Tef-1α gene primers. Of the ten Fusarium isolates collected from selected locations, six species were confirmed as F. acutatum and four as F. falciforme. The pathogenicity tests performed with onion seedlings and bulbs under moist conditions proved that both F. acutatum and F. falciforme independently could cause basal rot disease symptoms but with different degrees of virulence. Koch’s postulates were confirmed by reisolating the same pathogens from the infected plants. Thus, the active prevalence of FBR was confirmed in Maharashtra and also, to the best of our knowledge, this is the first report of F. falciforme and F. acutatum causing basal rot of onion independently in Maharashtra, India.

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“…Therefore, the isolates were observed to derive probably from the same clonal lineage. Moreover, the results revealed that geographical origin is not very important to make polymorphism within the isolates (Bayraktar, ; Galván et al., ; Malathi & Mohan, ).…”

Section: Discussionmentioning

confidence: 99%

Genetic and virulence variation in Fusarium oxysporum f. sp. cepae causing root and basal rot of common onion in Iran

Nasr-Esfahani

2018

Journal of Phytopathology

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Root and basal rot of common onion (Allium cepae L.) caused by Fusarium oxysporum f. sp. cepae is one of the most important diseases causing tremendous losses in onion‐growing areas worldwide. In this study, random amplified polymorphic DNA (RAPD), intersimple sequence repeats (ISSR) and virulence studies were conducted to analyse 26 F. oxysporum f. sp. cepae isolates obtained from the main onion‐growing regions of Iran, including Fars, Azerbaijan and Isfahan states. Cluster analysis using UPGMA method for both RAPD and ISSR markers revealed no clear grouping of the isolates obtained from different geographical regions, and the isolates were observed to derive probably from the same clonal lineage. Pathogenicity test indicated that all F. oxysporum f. sp. cepae isolates were pathogenic on onion; however, virulence variability was observed among the isolates. The grouping based on virulence variability was not correlated with the results of RAPD and ISSR analyses.

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Analysis of genetic variability ofFusarium oxysporumf. sp.cepaethe causal agent of basal rot on onion using RAPD markers

Cited by 4 publications

References 10 publications

Morphological, pathological and mycotoxicological variations among Fusarium verticillioides isolated from cereals

Morphological, pathological and mycotoxicological variations among Fusarium verticillioides isolated from cereals

Active Prevalence of Fusarium falciforme and F. acutatum Causing Basal Rot of Onion in Maharashtra, India

Genetic and virulence variation in Fusarium oxysporum f. sp. cepae causing root and basal rot of common onion in Iran

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