Analysis of genetic diversity of passion fruit (Passiflora edulis Sims) genotypes grown in Kenya by sequence-related amplified polymorphism (SRAP) markers

Characterization of the genetic diversity in Passiflora spp. in the Boyacá Department, Colombia

“…The average value of Nei-Li genetic diversity was 0.40, consistent with that expected for cross-pollinated species and with the values observed in studies on genetic diversity in Passiflora (Santos et al, 2011;Oluoch et al, 2018;Oliveira et al, 2019;Vianna et al, 2019). Ocampo et al (2017), when evaluating the diversity and genetic structure of P. edulis f. flavicarpa in Colombia with microsatellites, found an average genetic distance of 0.683 and that the structuring of the groups was not consolidated and could vary, just as in this study it was observed that the internal branches of the dendrogram were moderately compatible (bootstrap ≥ 50%) and ratified the grouping between some materials of the same department.…”

“…The AMOVA showed that 64% of the variation observed in the evaluated Passiflora materials was explained by the component within the groups, and 36% was explained by the differentiation between the groups, so it is necessary to consider subdivision levels and hierarchy greater than those contemplated in this study, in addition to the high levels of variation at the intraspecific level, which should be used for management and conservation strategies of this plant genetic resource (Table 4), which has also been reported in other genetic diversity studies on Passiflora using this marker (Ocampo et al, 2017;Oluoch et al, 2018;Fonseca-Trujillo et al, 2019). In interspecific analyses, a higher polymorphism rate in combination with a higher average number of bands amplified per primer is indicative of high genetic diversity (Cerqueira-Silva et al, 2012).…”

“…Knowledge on inter-and intraspecific genetic diversity provides useful information for effective conservation, management of plant genetic resources and breeding programs (Oluoch et al, 2018). Variability between genotypes is important for breeders in the selection of high-yield varieties, so the existence of variability among individuals is a prerequisite for the production of new varieties aimed at improving crop productivity, with the ability to resist the impacts of biotic or abiotic stress (Oliveira et al, 2019).…”

“…The genetic diversity in the Passiflora genus is very wide, both within the genus and within the most cultivated species (Vianna et al, 2019), so the characterization of the genetic variation is essential for the conservation, management and efficient use of germplasm in genetic breeding programs (Oluoch et al, 2018). Traditionally, diversity in Passiflora is estimated by observing variations in morphological or agronomic characteristics or tolerance to biotic or abiotic factors, which does not reflect the genetic relationship between evaluated materials and is affected by environmental changes and dependence on the stage of development (Pereira et al, 2015).…”

“…The genetic diversity in Passiflora has been studied with different molecular techniques, such as random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and sequence-related amplified polymorphism (SRAP) (Oluoch et al, 2018;Vieira et al, 2019); however, simple sequence repeat (SSR) markers are the most used techniques for the characterization of germplasm in different crops since they are highly polymorphic, reproducible and co-dominant, with wide genome coverage (Gramazio et al, 2018). Among the microsatellite markers, inter-simple sequence repeat (ISSR), which combines characteristics of the RAPD and microsatellites techniques, facilitates multilocus genomic analysis with a single primer based on regions of SSR, presents good reproducibility, has a low cost, is simple, does not need previous genome information (Morillo et al, 2018), and has proven to be polymorphic and informative in studies on genetic diversity in Passiflora (Santos et al, 2011;Sousa et al, 2015;Batista et al, 2017;Oliveira et al, 2019;Vianna et al, 2019).…”