Analysis of genetic diversity in cowpea (Vigna unguiculata L.Walp.) cultivars with random amplified polymorphic DNA markers

Malviya, Neha; Sarangi, Bijaya Ketan; Yadav, Manoj Kumar; Yadav, Dinesh

doi:10.1007/s00606-011-0545-9

Cited by 24 publications

(13 citation statements)

References 22 publications

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“…Genetic diversity information is extremely important for cowpea breeding which makes conservation and utilization easy for improvement of cultivars. The RAPD markers have proved to be a constructive tool in molecular characterization of cowpea cultivars (Prasanthi et al 2012;Malviya et al 2012;Pandey et al, 2004). Anatala et al (2014) compared ISSR and RAPD primers on genotypes of cowpea and found that ISSR and RAPD markers gave differentiated diversity to distinguished genotypes, individually with specific band.…”

Section: Explorations and Collectionmentioning

confidence: 99%

Cowpea genetic resources and its utilization: Indian perspective – A review

Tripathi

Gore

Ahlawat

et al. 2019

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Cowpea is a multipurpose food legume of the tropics and is a crucial source of nutrition for rural and tribal communities in Africa and Asia. High protein content, nitrogen-fixing ability, drought tolerance and adaptability to challenging environment make cowpea, a promising climate resilient food legume of 21st century. This crop is grown as sole or mix-crop and in agro-forestry combinations for diverse uses as pulse, vegetable and fodder. Although cowpea has been used by indigenous people for centuries, its full potential has never been realized. Its production did not increase over the years due to narrow genetic base, biotic and abiotic stresses and non-adaptation to changing climate. Ultimate solution lies in its genetic resource which are the reservoir of useful genes. Lack of information on germplasm characterization and evaluation restricts the effective use of germplasm stored in the genebanks. Introgression of genes from closely related Vigna species and application of modern molecular tools may facilitate broadening the genetic base of cowpea.

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Section: Explorations and Collectionmentioning

confidence: 99%

Cowpea genetic resources and its utilization: Indian perspective – A review

Tripathi

Gore

Ahlawat

et al. 2019

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“…with single sequence repeat (SSR) or microsatellites markers. The variation within and among cowpea populations from different agro‐ecological regions and germplasm accessions has been also evaluated using AFLP and RAPD markers . In addition, RAPD markers were used to eliminate the putative duplicates of Senegal cowpea accessions in a germplasm bank and identify elite varieties .…”

Section: Evaluation Of Genetic Diversitymentioning

confidence: 99%

Cowpea: a legume crop for a challenging environment

et al. 2017

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Cowpea is a grain legume native from Africa and is a primary source of protein for millions of people in sub-Saharan Africa and other parts of the developing world. The main important characteristics of this crop include a good protein quality with a high nutritional value, its nitrogen-fixing ability, and an ability to be more drought- and heat-tolerant than most of its legume relatives. In a research perspective, studies of cowpea are relatively scarce, despite its relevance to agriculture in the developing world and its resilience to stress. The present review provides an overview of different aspects of cowpea, with a special emphasis on the molecular markers for assessing genetic diversity, as well as on biochemical and transcriptomic data with respect to evaluating cowpea drought stress tolerance. The integration of both datasets will be useful for the improvement of cowpea because research on drought stress tolerance is of major interest for this crop in a challenging environment. © 2017 Society of Chemical Industry.

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“…The RAPD markers alone did not correlate with the morphological data to cluster the accessions, but when combined with the ISSR data a significant correlation was found. Malviya et al (2012) [22] used 18 sets of RAPD primers resulting in 148 polymorphic bands to analyse the genetic diversity among 10 Indian cultivars of cowpea. The detected relatively narrow genetic base was suggested to be due to a single domestication event occurring in the origin of this crop.…”

Section: Random Amplified Polymorphic Dnas (Rapds)mentioning

confidence: 99%

Molecular Markers for Genetic Diversity Studies in African Leafy Vegetables

Omondi¹,

Debener²,

Linde³

et al. 2016

ABB

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African leafy vegetables are becoming important crops in tackling nutrition and food security in many parts of sub-Saharan Africa, since they provide important micronutrients and vitamins, and help resource-poor farm families bridge lean periods of food shortage. Genetic diversity studies are essential for crop improvement programmes as well as germplasm conservation efforts, and research on genetic diversity of these vegetables using molecular markers has been increasing over time. Diversity studies have evolved from the use of morphological and biochemical markers to molecular markers. Molecular markers provide valuable data, since they detect mostly selectively neutral variations at the DNA level. They are well established and their strengths and limitations have been described. New marker types are being developed from a combination of the strengths of the basic techniques to improve sensitivity, reproducibility, polymorphic information content, speed and cost. This review discusses the principles of some of the established molecular markers and their application to genetic diversity studies of African leafy vegetables with a main focus on the most common Solanum, Amaranthus, Cleome and Vigna species.

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Analysis of genetic diversity in cowpea (Vigna unguiculata L.Walp.) cultivars with random amplified polymorphic DNA markers

Cited by 24 publications

References 22 publications

Cowpea genetic resources and its utilization: Indian perspective – A review

Cowpea genetic resources and its utilization: Indian perspective – A review

Cowpea: a legume crop for a challenging environment

Molecular Markers for Genetic Diversity Studies in African Leafy Vegetables

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