Analysis of genetic diversity in the crayfish plague fungus, Aphanomyces astaci, by random amplification of polymorphic DNA

Aphanomyces astaciPsI-genotype isolates from different Finnish signal crayfish stocks show variation in their virulence but still kill fast

“…The virulence variation of the crayfish plague (Aphanomyces astaci) strains from different epidemics had been long discussed (Huang et al, 1994;Fürst, 1995;Edgerton et al, 2004), until it was recently repeatedly documented (Makkonen et al, 2012bJussila et al, 2013a). In general, this disease has a bad reputation, but some A. astaci strains seem to show signs of avirulence (Jussila et al, 2011a;Makkonen, 2013).…”

Status of the white-clawed crayfish, Austropotamobius pallipes (Lereboullet, 1858), in Spain : distribution and legislation

“…The crayfish plague is the most serious disease affecting freshwater crayfish and it has cc wiped out ,, many populations of native crayfish in Europe. This disease is caused by the oomycetous fungus Aphanomyces astaci (UNESTAML~ 969), which is endemic to North America (UNESTAM, 1972 ;HUANG et a/., 1994 ;DIEGUEZ-URIBEONDO, 1995) and was probably introduced into Europe around 1860 (AL~ERMAN and POLGLASE, 1988). It is known that at least three different strains of this fungus have been introduced into Europe during the last three decades by the stocking with North American crayfish, i.e.…”

The Dispersion of the Aphanomyces Astaci-Carrier Pacifastacus Leniusculus by Humans Represents the Main Cause of Disappearance of the Indigenous Crayfish Austropotamobius Pallipes in Navarra

“…There are numerous studies showing the role of signal crayfish as a carrier of crayfish plague (see review CERENIUS and SODERHALL, 1999) and the relationship of this exotic crayfish to a large number of epizootics has been consistently proven (HUANG et al, 1994;LILLEY et al, 1997;VENNERSTRÖM et al, 1998; OIDTMANN…”

“…About 10 mg of fresh mycelium from PG-1 drop cultures were used for the DNA isolation. The PCR-reactions were carried out as described in HUANG et al, (1994) except that the primer concentration was 200 nM. Diagnostic primer B01 was used for analysis.…”

“…the signal crayfish Pacifastacus leniusculus (Dana, 1852), the spiny-cheek crayfish Orconectes limosus (Rafinesque 1817) and the swamp crayfish Procambarus clarkii (Girard, 1852), (PERSSON and SÖDERHÄLL, 1983;VEY et al, 1983;DIÉGUEZ-URIBEONDO et al, 1995). So far, three groups of A. astaci have been spread in Europe and named A, B and D. Group A is believed to represent isolates that originated from an early introduction of this parasite into Europe, group B consists of strains isolated from P. leniusculus or from outbreaks of indigenous European crayfish after introduction of this crayfish into Sweden in 1969 (HUANG et al, 1994); and group D includes strains isolated from P. clarkii in Spain (DIÉGUEZ- URIBEONDO et al, 1995).…”