Analysis of gene expression profiles under water stress in tolerant and sensitive sugarcane plants

Rodrigues, Fabiana Aparecida; Laia, Marcelo Luiz de; Zingaretti, Sônia Marli

doi:10.1016/j.plantsci.2008.11.007

Cited by 117 publications

(106 citation statements)

References 90 publications

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“…Results demonstrated stem reserve mobilization of sucrose at a higher level in ICSV213 compared to ICSB338 under stress as evident from the difference in brix and hexose levels between these varieties. These observations were previously made in soybean, pigeon pea and sugarcane mature culms under water stress (Lawlor and Cornic, 2002;Liu et al, 2004;Rodrigues et al, 2009 andIskandar et al, 2011). The higher levels of fructose produced in stressed plants in both varieties was an indicator that fructans were possibly the main storage carbohydrates remobilized to fructose under stress with suggestive roles in osmoregulation (Pilon-Smits et al, 1999;Livingston III et al, 2009;Gaudet et al, 2011 ;Van den Ende and El-Esawe, 2014).…”

Section: Discussionsupporting

confidence: 62%

The role of sucrose metabolizing proteins in hyperosmotic stress tolerance in sweet sorghum (Sorghum bicolor L. Moench)

Njokweni¹,

Ibraheem²,

Ndimba³

2016

Plant Omics

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Sugar accumulation is a common metabolic response under drought stress conditions. However, studies on the underlying molecular mechanisms of sugar accumulation under stress remain restricted. This study explores the role of sucrose metabolizing proteins in conferring tolerance to drought-induced hyperosmotic stress, and ultimately osmotic adjustment in sorghum. The effect of stress on sugar content, enzyme activity and gene expression was investigated. Sweet sorghum varieties (ICSV213 and ICSB338) differing in levels of drought tolerance were subjected to a 10-day water deficit period. Brix plant sap analysis indicated elevated total soluble sugar levels under water stress conditions in both varieties with ICSV213 demonstrating a higher brix content than ICSB338. HPLC analysis gave a decrease in sucrose levels and an increase in glucose and fructose concentrations in both varieties with ICSV213 demonstrating higher hexose levels. Enzyme activity levels of invertase, sucrose phosphate synthase and sucrose synthase were found to increase under stress in both varieties with ICSV213 invertase displaying the highest activity when compared to other sucrose metabolizing enzymes. Transcriptional expression of invertase and sucrose phosphate synthase (SPS) genes was significantly up regulated in ICSV213 under stressed conditions, whereas sucrose synthase (Susy) levels remained low in both varieties. Data obtained for sorghum variety ICSV213 points towards a relationship between hyperosmotic stress tolerance and the accumulation of solutes and sucrose metabolism proteins. Consequently variety ICSV213 may therefore be an excellent target for future development of hyperosmotic stress tolerant sweet sorghum.

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Section: Discussionsupporting

confidence: 62%

The role of sucrose metabolizing proteins in hyperosmotic stress tolerance in sweet sorghum (Sorghum bicolor L. Moench)

Njokweni¹,

Ibraheem²,

Ndimba³

2016

Plant Omics

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“…Aditionally, low NPQ has been reported to provide protection against photo-oxidative damage (Müller et al 2001;Omasa and Takayama 2003). In a recent study, a reduction in F v / F m and Φ PSII in some sugarcane cultivars was observed, and it resulted in reduced P n (Rodrigues et al 2009;da Silva et al 2012). Previously, P n has been demonstrated to be a very sensitive parameter for the classification of sugarcane germplasm for drought tolerance (de Silva and de Costa 2009; da ) and it has been found to hold good in the present study and useful in screening the sugarcane cultivars for drought tolerance.…”

Section: Chlorophyll Fluorescence and Photosynthetic Capacitymentioning

confidence: 97%

Evaluating sugarcane (Saccharum sp.) cultivars for water deficit tolerance using some key physiological markers

Cha–um

Wangmoon

Mongkolsiriwatana

et al. 2012

Plant Biotechnology

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Seven sugarcane (Saccharum sp.) commercial cultivars, viz., , originally derived from meristem cuttings were subjected to simulated osmotic stress (as 200 mM mannitol) under controlled environmental conditions. Proline content in the leaf tissues of all cultivars except K92-80, increased in plants subjected to mannitol-induced osmotic stress. Chlorophyll a (Chl a ), chlorophyll b (Chl b ), total chlorophyll (TC), maximum quantum yield of PSII (F v /F m ), and photon yield of PSII (Φ PSII ) of all seven cultivars decreased under osmotic stress resulting in a reduction in net-photosynthetic rate (P n ). A positive correlation was found between F v / F m and Φ PSII , proline content and non-photochemical quenching (NPQ), Φ PSII and P n , and P n and plant dry weight in the sugarcane cultivars. Based upon Ward's multivariate cluster analyses of data for proline content, photosynthetic capacity, chlorophyll fluorescence, and growth inhibition, three cultivars (K88-92, K92-80 and UT-94-2-483) were identified as water deficit sensitive, whereas four (K84-200, K95-84, K97-32 and LK92-11) as water deficit tolerant. These observations on different cultivar's sensitivity/tolerance were confirmed by growth and yield attributes measured in a field trial. The plant dry weight (in vitro) correlated positively with total stalk weight of sugarcane cultivars

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“…Plants that survive or maintain their growth rate in periods of water deficit are considered as tolerant (Rodrigues et al, 2009). When these changes occur, the root system is the first region in a plant to detect and signal the effects of stress to other cells, tissues, and organs (Sauter et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Proline accumulation in sugarcane roots subjected to drought conditions

Vantini¹,

Carlin²,

Gimenez³

et al. 2016

Científica

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Analysis of gene expression profiles under water stress in tolerant and sensitive sugarcane plants

Cited by 117 publications

References 90 publications

The role of sucrose metabolizing proteins in hyperosmotic stress tolerance in sweet sorghum (Sorghum bicolor L. Moench)

The role of sucrose metabolizing proteins in hyperosmotic stress tolerance in sweet sorghum (Sorghum bicolor L. Moench)

Evaluating sugarcane (Saccharum sp.) cultivars for water deficit tolerance using some key physiological markers

Proline accumulation in sugarcane roots subjected to drought conditions

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