Analysis of gene expression and enzyme activities related to starch metabolism in Lycoris sprengeri bulbs of different sizes

Chang, Le; Xiao, Yu; She, Lin Fang; Xia, Yi

doi:10.1016/j.scienta.2013.07.005

Cited by 28 publications

(20 citation statements)

References 24 publications

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“…As carbohydrate concentration increases, the activity levels of the beta enzyme increase significantly, but the activity levels of the alpha enzyme change only slightly (Chang et al, 2013). In addition, the soluble sugar content in the mature bulb correlates positively with the activity of the beta enzyme (Chang et al, 2013). The soluble sugar content, soluble protein content, oxidoreductase activity, and root activity of L. radiata differed significantly among various developmental stages across one growth season.…”

Section: Discussionmentioning

confidence: 93%

“…Previous studies have shown that as L. radiata bulb-lets (young bulbs) mature, bulb weight, diameter, starch content, root system activity, and zeatin riboside concentration correlate positively with the activity levels of the enzyme soluble starch synthase and bound starch synthetase (GBSS) (She et al, 2014). As carbohydrate concentration increases, the activity levels of the beta enzyme increase significantly, but the activity levels of the alpha enzyme change only slightly (Chang et al, 2013). In addition, the soluble sugar content in the mature bulb correlates positively with the activity of the beta enzyme (Chang et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A Three-dimensional Analysis of Summer Dormancy in the Red Spider Lily (Lycoris radiata)

Cai¹,

Fan²,

Wei³

et al. 2019

horts

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Lycoris radiata has beautiful bright-red flowers with both medicinal and ornamental value. However, the mechanisms underlying an unusual characteristic of Lycoris radiata, flowering without leaves, remain unclear. In this study, climatic influences, biomass composition, and yearly variations in bulb contents across eight developmental stages of L. radiata were analyzed. Thus, L. radiata summer dormancy was investigated in three dimensions: climate-associated phenology, biomass distribution characteristics, and physiologic bulb changes. The results showed that dormancy was most strongly affected by high ambient temperature, followed by scape development, flowering, leafing out, vigorous leaf growth, flower bud differentiation, flower bud predifferentiation, and leaf maturation. Biomass allocation, bulb contents, oxidoreductase activity, and root activity fluctuated significantly in L. radiata among developmental stages. Relative bulb dry weight was greatest during the dormant period (95.95% of total dry weight) and lowest during vigorous leaf growth (November–December). Root biomass was also significantly greater during dormancy than during flowering, leaf maturation, and flower bud differentiation. Only root biomass during vigorous leaf growth was greater than root biomass during dormancy. However, in dormant bulbs, soluble sugar content, soluble protein content, root activity, superoxide dismutase (SOD) activity, and peroxidase (POD) activity decreased. Thus, summer dormancy in L. radiata only constitutes a morphologic dormancy of the aboveground plant; the bulb and root remain physiologically active. The results suggest that L. radiata is sensitive to both ambient temperature and light, and that summer dormancy is triggered by the synergistic stimulation of these two factors. Although temperature controls dormancy, it plays only a limited regulatory role during the L. radiata flowering period. Thus, it is difficult to induce flowering or regulate annual flowering in this species through temperature control alone.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

A Three-dimensional Analysis of Summer Dormancy in the Red Spider Lily (Lycoris radiata)

Cai¹,

Fan²,

Wei³

et al. 2019

horts

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“…Over the developmental stages of freesia corms, sucrose and starch exhibited an overall increasing trend, in agreement with the results reported on Lilium davidii var. unicolor [7] and Lycoris sprengeri bulbs [20]. At the formation stage, levels of sucrose and soluble sugars and starch were relatively low owing to the great nutritional need from mother corms for leaf development, and great photosynthate need for aerial biomass, allowing less carbohydrates allocated to new corms.…”

Section: Discussionmentioning

confidence: 99%

Dynamics and Transcriptome Analysis of Starch and Sucrose Metabolism During Corm Development in Freesia Hybrida

Su-Qin

Yan

et al. 2020

Preprint

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Background Starch and sucrose metabolism plays a crucial role in the formation and development of bulbs in bulbous plants, which, however, remains unclear and unexplored in the corms of Freesia hybrida, one kind of famous bulbous flower. Herein, we investigated the dynamics of the major form of carbohydrates and related enzyme activities and profiled the transcriptome of freesia corms at four developmental stages with the aim to reveal the relation of starch and sucrose metabolism to corm development and the transcriptional regulation of this metabolic process.Results The content of starch, sucrose and soluble sugars followed an overall upward trend across the corm developmental stages. Activities of the adenosine diphosphoglucose pyrophosphorylase, starch branching enzyme and β-amylase generally followed the pattern of the starch and sucrose level. Activities of sucrose phosphate synthase increased from corm formation till the initial swelling stage and subsequently reached a plateau. Activities of invertase and sucrose synthase peaked at the later rapid swelling stage. Transcriptome analysis revealed a total of 100,999 unigenes, out of which 44,405 unigenes were annotated. Analysis based on Clusters of Orthologous Groups suggested that carbohydrate transport and metabolism (9.34% of the sequences) was prominent across the corm developmental process. Totally 3427 differentially expressed genes were identified and the enrichment analysis directed starch and sucrose metabolism a critical pathway in corm development especially at the rapid swelling stage. Genes encoding key carbohydrate-metabolizing enzymes were identified and their differential expression related to corm development was explored.Conclusions The comparative transcriptome analysis discloses essential roles of starch and sucrose metabolism and the genetic mechanism related to the corm development. The results construct a valuable resource pool for further molecular-level studies, which are helpful for metabolic regulation of carbohydrates and improvement in molecular breeding of Freesia hybrida.

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“…Granule-bound starch synthase (GBSS) can specifically elongate amylose (Nelson and Rines, 1962) while soluble starch synthase (SSS) is exclusively involved in amylopectin biosynthesis (Streb and Zeeman, 2012). We have previously reported that bulb formation and swelling are closely correlated with carbohydrates manipulation between aboveground and underground parts either in the lily or Lycoris under open field conditions (Zheng et al, 2011;Chang et al, 2013;She et al, 2014). Comparative carbohydrate-related genes involved in bulblet formation and development were examined in Lilium davidii var.…”

Section: Introductionmentioning

confidence: 99%

“…In the current paper we selected Lilium as our model material for its high reproductive efficiency compared with the tulip or Lycoris (Alderson et al, 1982;Kuijpers and Langens-Gerrits, 1997;Chang et al, 2013). We focused solely on starch metabolism linked to autotrophic organ carbon reserve conversion.…”

Section: Introductionmentioning

confidence: 99%

Low humic acids promote in vitro lily bulblet enlargement by enhancing roots growth and carbohydrate metabolism

Xia

Zhang

et al. 2016

J. Zhejiang Univ. Sci. B

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Bulblet development is a problem in global lily bulb production and carbohydrate metabolism is a crucial factor. Micropropagation acts as an efficient substitute for faster propagation and can provide a controllable condition to explore bulb growth. The present study was conducted to investigate the effects of humic acid (HA) on bulblet swelling and the carbohydrate metabolic pathway in Lilium Oriental Hybrids 'Sorbonne' under in vitro conditions. HA greatly promoted bulblet growth at 0.2, 2.0, and 20.0 mg/L, and pronounced increases in bulblet sucrose, total soluble sugar, and starch content were observed for higher HA concentrations (≥2.0 mg/L) within 45 d after transplanting (DAT). The activities of three major starch synthetic enzymes (including adenosine 5'-diphosphate glucose pyrophosphorylase, granule-bound starch synthase, and soluble starch synthase) were enhanced dramatically after HA application especially low concentration HA (LHA), indicating a quick response of starch metabolism. However, higher doses of HA also caused excessive aboveground biomass accumulation and inhibited root growth. Accordingly, an earlier carbon starvation emerged by observing evident starch degradation. Relative bulblet weight gradually decreased with increased HA doses and thereby broke the balance between the source and sink. A low HA concentration at 0.2 mg/L performed best in both root and bulblet growth. The number of roots and root length peaked at 14.5 and 5.75 cm, respectively. The fresh bulblet weight and diameter reached 468 mg (2.9 times that under the control treatment) and 11.68 mm, respectively. Further, sucrose/starch utilization and conversion were accelerated and carbon famine was delayed as a result with an average relative bulblet weight of 80.09%. To our knowledge, this is the first HA application and mechanism research into starch metabolism in both in vitro and in vivo condition in bulbous crops.

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Analysis of gene expression and enzyme activities related to starch metabolism in Lycoris sprengeri bulbs of different sizes

Cited by 28 publications

References 24 publications

A Three-dimensional Analysis of Summer Dormancy in the Red Spider Lily (Lycoris radiata)

A Three-dimensional Analysis of Summer Dormancy in the Red Spider Lily (Lycoris radiata)

Dynamics and Transcriptome Analysis of Starch and Sucrose Metabolism During Corm Development in Freesia Hybrida

Low humic acids promote in vitro lily bulblet enlargement by enhancing roots growth and carbohydrate metabolism

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