Analysis of gene and protein expression during monocyte-macrophage differentiation and cholesterol loading—cDNA and protein array study

Tuomisto, Tiina T.; Riekkinen, Mervi S.; Viita, Helena; Levonen, Anna–Liisa; Ylä‐Herttuala, Seppo

doi:10.1016/j.atherosclerosis.2004.12.023

Cited by 62 publications

(46 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…8). CD14 is a specific surface receptor known to be expressed on macrophages (Tuomisto et al 2005). The expression of CD14 at the protein level has yet to be established.…”

Section: Resultsmentioning

confidence: 99%

Metformin induced expression of Hsp60 in human THP-1 monocyte cells

Tsuei

Martinus

2012

Cell Stress and Chaperones

View full text Add to dashboard Cite

Metformin is in widespread clinical use for the treatment of diabetes mellitus in patients. It has been shown to inhibit mitochondrial bioenergetic functions by inhibiting complex I of the electron transport chain. The expression of mitochondrial-specific molecular stress protein Hsp60 is a key consequence of mitochondrial impairment. Since this protein has important immune-modulatory properties, we have investigated the expression of Hsp60 in human THP-1 monocyte cells exposed to metformin. In this study, we demonstrate significant up-regulation of Hsp60 at both mRNA and protein levels when these cells were exposed to metformin at therapeutic dosage levels. Interestingly, there was also an increase in expression of CD14 mRNA in these cells. This suggested a possible modulation of the differentiation rates of the THP-1 cells during exposure to metformin. As monocyte differentiation marks a critical step in atherosclerosis, these observations suggest that longterm exposure to metformin could have important implications for the diabetic patient.

show abstract

“…8). CD14 is a specific surface receptor known to be expressed on macrophages (Tuomisto et al 2005). The expression of CD14 at the protein level has yet to be established.…”

Section: Resultsmentioning

confidence: 99%

Metformin induced expression of Hsp60 in human THP-1 monocyte cells

Tsuei

Martinus

2012

Cell Stress and Chaperones

View full text Add to dashboard Cite

show abstract

“…The main LDL modifications related to atherosclerosis development involve lipid peroxidation 5. Transcriptional response of macrophages to exposure to oxidized (ox) LDL has been tested in cultured macrophages of different sources, with diverse results that, depending on the study, suggested predominantly proinflammatory or anti‐inflammatory effects 6, 7, 8, 9. In some cases, the outcome was significantly affected by changes in experimental variables, such as the cell type, form of LDL modification and presentation to cells, or time of exposure to the lipoproteins.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Profiling of Foam Cells Reveals Induction of Guanylate‐Binding Proteins Following Western Diet Acceleration of Atherosclerosis in the Absence of Global Changes in Inflammation

Goo

Son

Yechoor

et al. 2016

JAHA

View full text Add to dashboard Cite

BackgroundFoam cells are central to two major pathogenic processes in atherogenesis: cholesterol buildup in arteries and inflammation. The main underlying cause of cholesterol deposition in arteries is hypercholesterolemia. This study aimed to assess, in vivo, whether elevated plasma cholesterol also alters the inflammatory balance of foam cells.Methods and ResultsApolipoprotein E–deficient mice were fed regular mouse chow through the study or were switched to a Western‐type diet (WD) 2 or 14 weeks before death. Consecutive sections of the aortic sinus were used for lesion quantification or to isolate RNA from foam cells by laser‐capture microdissection (LCM) for microarray and quantitative polymerase chain reaction analyses. WD feeding for 2 or 14 weeks significantly increased plasma cholesterol, but the size of atherosclerotic lesions increased only in the 14‐week WD group. Expression of more genes was affected in foam cells of mice under prolonged hypercholesterolemia than in mice fed WD for 2 weeks. However, most transcripts coding for inflammatory mediators remained unchanged in both WD groups. Among the main players in inflammatory or immune responses, chemokine (C‐X‐C motif) ligand 13 was induced in foam cells of mice under WD for 2 weeks. The interferon‐inducible GTPases, guanylate‐binding proteins (GBP)3 and GBP6, were induced in the 14‐week WD group, and other GBP family members were moderately increased.ConclusionsOur results indicate that acceleration of atherosclerosis by hypercholesterolemia is not linked to global changes in the inflammatory balance of foam cells. However, induction of GBPs uncovers a novel family of immune modulators with a potential role in atherogenesis.

show abstract

“…CD36 is a major macrophage scavenger receptor for oxidised low-density lipoprotein (LDL) particles [1] and is believed to play a critical role in the initiation and progression of atherosclerosis through its ability to bind and internalise modified LDL, facilitating formation of lipid-engorged macrophage foam cells [2][3][4]. In addition, the 7q chromosome region, containing the CD36 gene, has been linked to components of metabolic syndrome in several genome-wide association studies [5,6].…”

Section: Introductionmentioning

confidence: 99%

Is plasma-soluble CD36 associated with density of atheromatous plaque and ankle–brachial index in early-onset coronary artery disease patients?

et al. 2016

View full text Add to dashboard Cite

A b s t r a c tBackground: CD36 is a major macrophage scavenger receptor for oxidised low-density lipoprotein particles. Soluble CD36 (sCD36) is circulating as a ligand-bound complex and may be present in microparticles shed from cells such as platelets, monocytes/macrophages, or adipocytes. Positive association of plasma sCD36 with insulin resistance has been reported, and it has been proposed that sCD36 might represent a marker of macrophage activation and inflammation leading to atherosclerosis. Recently we have identified an association between CD36 polymorphism and low thickness of atheromatous plaque, suggesting its protective effect against atherosclerosis development. Aim:To obtain insight into the relationship between plasma concentration of sCD36 and radiological parameters of atherosclerosis in patients with early-onset coronary artery disease (CAD). Methods:The study group comprised 70 clinically stable patients (18 women and 52 men) with early CAD (aged no more than 50 years for men and 55 years for women). Fasting blood sample was taken for serum glucose, lipid profile, ApoA1, ApoB, Lp(a), and plasma sCD36 protein measurements. Each subject's weight, height, waist and hip circumference, and systolic and diastolic blood pressure were measured, and the body mass index, waist-to-hip ratio, and mean arterial pressure were calculated. Doppler ultrasound examinations of carotid and peripheral arteries were performed in all patients. Thickness of intima-media complex (IMC) of common carotid (CCA) and brachial arteries, as well as density and thickness of atheromatous plaque at CCA bifurcation, were measured with M'Ath programme. Plasma concentrations of CD36 antigen were measured by ELISA. Correlations between quantitative variables and sCD36 plasma concentration were assessed with the Spearman rank correlation coefficient (Rs). Associations between qualitative variables and sCD36 plasma concentration were tested with the Mann-Whitney U test. Results:We observed no significant correlations between sCD36 concentration and radiological parameters of atherosclerosis. We found only borderline significant negative correlation of sCD36 concentration with thickness of IMC of left brachial artery. We also observed a significantly negative correlation with CCA plaque density, but only in the female subgroup and on the right side. Borderline higher sCD36 plasma concentrations were observed in patients with lower ankle-brachial index value (< 0.9). Conclusions:The results of the study show no strong associations and do not prove either detrimental or beneficial influence of sCD36 on radiological parameters of atherosclerosis. Further research is necessary to assess the association of high plasma sCD36 concentrations with the risk of plaque instability in patients with early-onset CAD.

show abstract

Analysis of gene and protein expression during monocyte-macrophage differentiation and cholesterol loading—cDNA and protein array study

Cited by 62 publications

References 34 publications

Metformin induced expression of Hsp60 in human THP-1 monocyte cells

Metformin induced expression of Hsp60 in human THP-1 monocyte cells

Transcriptional Profiling of Foam Cells Reveals Induction of Guanylate‐Binding Proteins Following Western Diet Acceleration of Atherosclerosis in the Absence of Global Changes in Inflammation

Is plasma-soluble CD36 associated with density of atheromatous plaque and ankle–brachial index in early-onset coronary artery disease patients?

Contact Info

Product

Resources

About