Analysis of Fructans from Higher Plants by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Stahl, Bernd; Linos, Alexandros; Karas, Michael; Hillenkamp, Franz; Steup, Martin

doi:10.1006/abio.1997.2011

Cited by 101 publications

(74 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1), while Stahl et al . 25) used simultaneous MALDI MS and HPAEC methods and obtained similar results. These advanced techniques led to an ideal separation and identification of the different FOS found in onion bulb tissues as shown in Table 1.…”

Section: Fructooligosaccharides In Onion Bulbsmentioning

confidence: 71%

The Metabolism of the Fructooligosaccharides in Onion Bulbs: A Comprehensive Review

2005

View full text Add to dashboard Cite

In the past two decades, there has been vast expansion in the research of fructooligosaccharides (FOS), including their chemistry, biochemistry and enzymology in higher plants. However, in spite of these considerable advances in fructan science, many other aspects of the mechanisms of fructan metabolism have not been fully understood. The bulbing and yield of dry onion cultivars vary, but they depend on the contents of the high dry matter and non-structural carbohydrates (fructooligosaccharides) which contribute to keepability. The knowledge of the mechanisms of the synthesis and the degradation of the FOS occurring during growth and storage are of great interest. Important progress has been made in the research area of onion FOS, and in addition to their role as accessible reserve carbohydrates in bulbs during sprouting, FOS participate in many physiological processes of production, protection and preservation of the bulbs. This review aims to contribute to a better understanding of the metabolism of FOS in onion bulbs, based on recent investigations. The activities of the main enzymes involved in the synthesis and the hydrolysis of the FOS during the pre-harvest (growth) and post-harvest (storage) life of the bulbs are reviewed, although present knowledge is too limited to explain clearly the mechanisms triggering the enzymes activities or the mechanisms by which FOS contribute to quality and long keepability of the bulbs.

show abstract

Section: Fructooligosaccharides In Onion Bulbsmentioning

confidence: 71%

The Metabolism of the Fructooligosaccharides in Onion Bulbs: A Comprehensive Review

2005

View full text Add to dashboard Cite

show abstract

“…For example, MALDI-TOF has been recently used for the direct profiling of peptides and proteins in single cells or tissues [15][16][17][18]. However, since LDI-TOF is primarily a surfaceprobing tool, e.g., ions are generated only from the exterior regions of the sample that are exposed to laser irradiation [19], determination of intracellular contents usually requires special sample preparation [15].…”

mentioning

confidence: 99%

In-situ probing of the biotic-abiotic boundary of plants by laser desorption/ionization time-of-flight mass spectrometry

Sluszny

Yeung

Nikolau

2005

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

Laser desorption/ionization time-of-flight (LDI-TOF) mass spectrometry was applied for the direct analysis of cuticular waxes on intact plant tissues. Cuticular wax compounds were ionized by laser desorption in the presence of colloidal silver. Silver-adduct ions were detected on samples from Arabidopsis thaliana and from maize. Good spot-to-spot reproducibility indicated homogeneous coverage of the sample by the fine colloidal material. The results were consistent with GC-MS analyses of cuticular extracts, thus confirming the feasibility of direct analysis based on this protocol. Molecular masses of the adduct ions correspond well with the known composition of cuticular waxes. Moreover, LDI-TOF gave good estimates of the relative local abundances of a given compound. However, bias was found in cases where compounds with different ionization efficiencies were analyzed. (J Am Soc Mass Spectrom 2005, 16, 107-115)

show abstract

“…The instrument was operated at an accelerating voltage of 30 kV and an extractor voltage of 9 kV. The pressure was ~2.1 x 10 -6 Torr (Stahl et al, 1997).…”

Section: Maldi-tof-ms Analysismentioning

confidence: 99%

Characterization of fructans from Agave durangensis

Orozco-Cortes¹,

Álvarez-Manilla²,

Gutiérrez-Sánchez³

et al. 2015

Afr. J. Plant Sci.

View full text Add to dashboard Cite

Analysis of Fructans from Higher Plants by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Cited by 101 publications

References 31 publications

The Metabolism of the Fructooligosaccharides in Onion Bulbs: A Comprehensive Review

The Metabolism of the Fructooligosaccharides in Onion Bulbs: A Comprehensive Review

In-situ probing of the biotic-abiotic boundary of plants by laser desorption/ionization time-of-flight mass spectrometry

Characterization of fructans from Agave durangensis

Contact Info

Product

Resources

About