Analysis of forensic genetic parameters of 22 autosomal STR markers (PowerPlex® Fusion System) in a population sample from Bosnia and Herzegovina

Contemporary population genetics data for 23 Y-STR loci in the general Bosnian-Herzegovinian population

“…Our study was prepared according to the latest guidelines on publishing forensic and population genetics data for Y-STRs (Carracedo et al, 2014), which state that a minimum of 17 Y-STR loci and 200 samples should be used. Autosomal STR data have already been updated in previous years, firstly by publishing data on 1000 samples analysed for 15 STR loci contained in the PowerPlex 16 system (Promega Corporation;Pilav et al, 2017), followed by publishing data on 22 STR loci contained in the PowerPlex Fusion system (Promega Corporation) on a sample of 600 Bosnian-Herzegovinians (Pilav et al, 2020). The need for an increased sample size and improved data resolution for Y-chromosome studies is addressed in the present study.…”

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“…Considering all aforementioned, knowledge about mutation rates and possible mutational processes of different STR loci is important for accurate genetic profiles interpretation (Qian et al, 2015;Hamester et al, 2019). As previously mentioned in the Introduction section, a number of population-genetic studies, focused on evaluating of forensic efficiency parameters of STR markers (Grskovic et al, 2013 2016; Crnjac et al, 2017;Veselinovic et al, 2018;Al-Eitan et al, 2019;Al-Eitan et al, 2020;Pilav et al, 2020;Takic Miladinov et al, 2020) indicated a high degree of reliability of these markers important for all kinds of forensic-genetic analyses, including kinship and parentage testing. Finally, results from these two cases clearly demonstrate that X-STR markers have the potential to solve parentage cases not easily solved by standard analysis based on autosomal STR markers.…”

“…Besides high heterozygosity, discriminating power, clearly defined repetitive units and precisely determined allelic variants, simple amplification and detection of STR markers make them ideal for forensic analyses (Khalil et al, 2008;Primorac et al, 2014;Gomes et al, 2020). STR markers located on the autosomal, X and Y chromosomes are highly informative, practical and reliable for all kinds of forensic-genetic analyses including parentage testing, what was strongly supported by many population-genetic studies focused on investigating allele frequencies and forensic parameters (such as heterozygosity, power of discrimination, power of exclusion, polymorphic information content, matching probability and typical paternity index) of autosomal STR (Al-Eitan and Tubaishat, 2016;Al-Eitan et al, 2019;Al-Eitan et al, 2020;Pilav et al, 2020;Takic Miladinov et al, 2020) and X-STR loci (Grskovic et al, 2013;Crnjac et al, 2017;Veselinovic et al, 2018) in different populations. Generally, parentage testing follows Mendelian inheritance law, according to which child receives one allele from the mother and the other allele from the father .…”

DNA Paternıty Testıng wıth Two Mısmatches: Our Experıence

“…Besides high heterozygosity, discriminating power, clearly defined repetitive units and precisely determined allelic variants, simple amplification and detection of STR markers make them ideal for forensic analyses (Khalil et al, 2008;Primorac et al, 2014;Gomes et al, 2020). STR markers located on the autosomal, X and Y chromosomes are highly informative, practical and reliable for all kinds of forensic-genetic analyses including parentage testing, what was strongly supported by many population-genetic studies focused on investigating allele frequencies and forensic parameters (such as heterozygosity, power of discrimination, power of exclusion, polymorphic information content, matching probability and typical paternity index) of autosomal STR (Al-Eitan and Tubaishat, 2016;Al-Eitan et al, 2019;Al-Eitan et al, 2020;Pilav et al, 2020;Takic Miladinov et al, 2020) and X-STR loci (Grskovic et al, 2013;Crnjac et al, 2017;Veselinovic et al, 2018) in different populations. Generally, parentage testing follows Mendelian inheritance law, according to which child receives one allele from the mother and the other allele from the father .…”