Analysis of FMRFamide-like peptide (FLP) diversity in phylum Nematoda

Anthelmintic drugs and nematicides: studies in Caenorhabditis elegans

“…Thus anthelmintic resistance screens can promote the discovery and characterisation of genes that have important roles in neurotransmission. An excellent example of the utility of this approach is provided by genetic screens employing the organophosphate cholinesterase inhibitors, in particular aldicarb which provides an excellent example of 'genetic pharmacology' and the interested reader is directed to Nguyen et al (1995), Miller et al (1996), and Sieburth et al (2005.…”

FMRFamide‐like peptides encoded on the flp‐18 precursor gene activate two isoforms of the orphan Caenorhabditis elegans G‐protein‐coupled receptor Y58G8A.4 heterologously expressed in mammalian cells

“…Many of the C. elegans peptides are identical or highly homologous to those isolated or predicted in parasitic nematodes, including Ascaris suum, Haemonchus contortus, Ancylostoma caninum, Heterodera glycines, and Meloidogyne arenaria, suggesting that the function of these peptides is similar across species. 4,5 Neuropeptide-receptor matching is needed to gain a better understanding of the biological function of these systems in situ. If that function is found essential for the maintenance of the parasite in the host, the receptor defined highthroughput screens could be established for finding nonpeptidic small molecules, either receptor agonists or antagonists, as potential antiparasitic drug candidates.…”

Peptidomics in Neuroendocrine Research: A Caenorhabditis elegans and Mus musculus Study

“…Later on, five additional flp genes, flp-24 to flp-27 and flp-32 were reported by EST data mining and BLAST analysis [19], while peptides from flp-24 and flp-26 were independently sequenced by a peptidomics analysis at that time [20]. Members form the so-called neuropeptide-like gene family in C. elegans, were initially identified using bioinformatic programs like BLAST and PATTERNFIND to search the genomic sequence.…”

“…Later, one additional novel insulin-like gene, daf-28, was reported [23]. Taking all together, a huge amount of 109 neuropeptide genes have been identified to date using bioinformatics and peptidomics [18][19][20][21].…”