Analysis of fluoroquinolone-resistance using MIC determination and homology modelling of ParC of contemporary Mycoplasma genitalium strains

Prevalence of macrolide and fluoroquinolone resistance-associated mutations in Mycoplasma genitalium in metropolitan and overseas France

“…P values compare prevalence between men and women.*The percentages of macrolide resistance-associated mutations calculated among the 13 centres that participated in the 3 years of the study were 33.1% (56/169) in 2018, 34.8% (81/233) in 2019 and 36.4% (60/165) in 2020 (p=0.82).†Significant increase in the prevalence of macrolide resistance-associated mutations between 2018 and 2019. The percentages of macrolide resistance-associated mutations calculated among the three overseas territories that participated both years of the study were 7.3% (14/191) in 2018 and 14.7% (38/258) in 2019 (p=0.01).‡Only parC Asp87Tyr, Asp87Asn, Ser83Ile, Ser83Arg and Gly81Cys alterations, which are likely to have clinical significance, were counted 9–15 23§The percentages of ParC amino acid changes calculated among the 13 centres that participated in the 3 years of the study were 15.4% (27/175) in 2018, 15.3% (40/262) in 2019 and 17.9% (24/134) in 2020 (p=0.77).¶The percentage of dual macrolide resistance-associated mutations and ParC amino acid changes likely of clinical significance calculated among the 13 centres that participated in the 3 years of the study were 9.7% (14/145) in 2018, 10.6% (20/189) in 2019 and 13.1% (16/122) in 2020 (p=0.65).QRDR, quinolone resistance-determining region; ND, not determined.…”

“…‡Only parC Asp87Tyr, Asp87Asn, Ser83Ile, Ser83Arg and Gly81Cys alterations, which are likely to have clinical significance, were counted 9–15 23…”

“…The prevalence of amino acid changes in the QRDR of the ParC protein is presented in table 3. The Ser83Ile, Ser83Arg, Asp87Tyr, and Asp87Asn changes were considered likely of clinical significance because increased Minimum Inhibitory concentrations (MICs) have been observed in mutated isolates9 10 or because moxifloxacin treatment failures were reported in the presence of these mutations 11–14. The Gly81Cys mutation was also considered significant because increased moxifloxacin MIC were reported 15.…”

“…The Gly81Cys mutation was also considered significant because increased moxifloxacin MIC were reported 15. In contrast, the ParC Ser83Asn alteration, which did not significantly increase fluoroquinolone MICs,10 was not included in the ParC alterations likely to have clinical significance.…”

“…* Mycoplasma genitalium numbering with Echerichia coli numbering in parentheses. Only ParC Asp87Tyr, Asp87Asn, Ser83Ile, Ser83Arg and Gly81Cys alterations, which are likely to have clinical significance, were counted 9–15 23QRDR, quinolone resistance-determining region.…”