Analysis of fluorescence collection efficiency for fiber optic scanning two-photon endomicroscopy

Abstract

Piezoelectric ceramic tube fiber-scanning two-photon endomicroscopy is an essential division of miniature two-photon microscopy. The reverse collection optical path of the two-photon endomicroscopy platform is modeled and designed in this study. After simulating the chromatic aberration characteristics of the objective, the effects of the collection signal wavelengths, off-axis positions, fiber cladding diameters, and imaging depths on the collection efficiency are evaluated using Monte Carlo simulation. The results provide an additional theoretical explanation for enhancing the two-photon endomicroscopy platform's imaging sensitivity and signal-to-noise ratio.